RESUMEN
Objective To investigate the interfering efficiency of RNAi technique on the expression of Bcl-2 oncogene in human oral adenoid cystic carcinoma (Acc-3/CDDP).Methods The recombined RNAi plasmids for Bcl-2 oncogene were constituted by the four successive steps-designing of Oligo DNAs, synthesis of Oligo DNAs, transfection of Oligo DNAs into pSUPER.neo+gfp vectors and selection of positive plasmids. In order to silence the expression of Bcl-2 oncogenes, the recombined RNAi plasmids were transfected into Acc-3/CDDP cells by culturing together for about 10 hours, and the interfering efficiency of RNAi for the two oncogenes was evaluated by fluorescence-quantitative RT-PCR.Results The interfering efficiencies for Bcl-2 oncogene were 0,66.20% and 0, respectively in psiB1、siB2、psiB3.Conclusions The recombined RNAi plasmids of psiB2 (CCgggAgATAgTgATgAA) for Bcl-2 oncogene can effectively silence the expression of Bcl-2 oncogenes in Acc-3/CDDP cell line.
RESUMEN
Objective: To investigate the interfering efficiency of RNAi technique on the expression of c-erbB-2 and Bcl-2 oncogene in human tongue squamous carcinoma(Tca8113).Methods: The recombined RNAi plasmids for c-erbB-2 and Bcl-2 oncogene were constituted by the four succussive steps-designing of Oligo DNAs,synthesis of Oligo DNAs,transfection of Oligo DNAs into pSUPER.neo+gfp vectors and selection of positive plasmids.In order to silence the expression of c-erbB-2 and Bcl-2 oncogenes,the recombined RNAi plasmids were transfected into Tca8113 cells by culturing together for about 10 hours,and the interfering efficiency of RNAi for the two oncogenes was evaluated by fluorescence-quantitative RT-PCR.Results: The interfering efficiencies for c-erbB-2 oncogene were 62.68% and 40.61%,respectively in psiC1?psiC2?psiC3,while the interfering efficiencies for Bcl-2 oncogene were 66.20% and respectively in psiB1?siB2?psiB3.Conclusion: The recombined RNAi plasmids of psiC2(gAgTCCCAACCATgTCAAA) for c-erbB-2 oncogene and of psiB2(CCgggAgATAgTgATgAA) for Bcl-2 oncogene can effectively silence the expression of c-erbB-2 and Bcl-2 oncogenes in Tca8113 cell.