RESUMEN
Objective To study the HPLC fingerprint methodology of Rupikang Capsules. Methods The precision, stability and repeatability of the fingerprint of Rupikang Capsules were researched by HPLC. The HPLC system:Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5μm);the mobile phase:water-0.1% phosphoric acid water-acetonitrile, with linear gradient elution;the flow rate:1 mL/min;the detection wavelength:280 nm.Results The precision, stability and repeatability of Rupikang Capsules met the requirements of HPLC fingerprint technology. Conclusion This method is simple, feasible and can be used as the whole quality control method of Rupikang capsules.
RESUMEN
Objective To establish the quality standard for Rupikang Capsules. Methods Lumbricus were identified by TLC. Tanshinone IIA was determined by HPLC. HPLC analysis was carried out on Hitachi L-2000 C18 column (250 mm×4.6 mm, 5 μm) and the mixture of methanol-water (75∶25) was used as the mobile phase, the flow rate was 1.0 mL/min and the detection wavelength was at 270 nm. Results TLC spots developed were fairly clear and the blank test showed no interference. The linear range was 0.16-0.4 μg for tanshinone ⅡA, r=0.999 9. The average recovery was 99.1%, RSD=2.20%. Conclusion The methods is easy to operate, with good specificity and reproducibility. It can be used for the quality control of Rupikang Capsules.