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Objective To analyze the expression of toll like receptor 2/4(TLR2,TLR4)in peripheral blood mononuclear cells (PBMC)cells of children with henoch schonlein purpura(HSP),and investigate its relationship with T-cell subgroups. Methods A total of 85 HSP children treated in Shanghai Children's Medical Center from March 2015 to February 2017 were selected,including 49 children without renal failure(HSP group)and 16 children with renal failure(HSPN group).30 healthy children were enrolled as control group.The expression of TLR2,TLR4 was examined by real-time PCR.The ratio of Th1,Th2,Th17,Treg,Th1/Th2 and Th17/Treg was detected by flow cytometry.The relationship between TLR2,TLR4 and Th1/Th2,Th17/Treg was analyzed.Results The expression of TLR2 in HSPN,HSP and control group were 2.13± 0.36,1.64±0.27 and 1.05±0.12,the expression of TLR4 were 2.21±0.45,1.82±0.34 and 1.13±0.17 respectively.The differences were statistically significant(F=129.630,81.241,all P<0.01).The percentage of Th1,Treg cells and the ratio of Th1/Th2 in HSPN group were significantly lower than those in HSP group and control group,and the differences were statistically significant(F=34.103,63.828,91.432,all P<0.01).The percentage of Th2,Th17 cells and the ratio of Th17/Treg in HSPN group were significantly lower than those in HSP group and control group,and the differences were statisti-cally significant(F=48.559,48.475,34.909,P<0.01).The expression of TLR2 and TLR4 were negatively correlated with Th1/Th2(r=-0.601,-0.652,P<0.01),and positively correlated with Th17/Treg(r=0.617,0.712,P<0.01).Conclu-sion The expression of TLR2,TLR4 increases in HSP children,and may be related to the imbalance of Th1/Th2 and Th17/Treg immune response.
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Objective To investigate the effect of combined use of acupuncture and medicine on T cell subgroups in ulcerative colitis patients. Methods Sixty-four patients with ulcerative colitis were randomly allocated to treatment and control groups, 32 cases each. The treatment group received acupuncture plus medication and the control group, medication alone. After two months of treatment, changes in T cell subgroups were observed in the two groups and the clinical therapeutic effects were compared between the two groups. Results The total efficacy rate was 87.5% in the treatment group and 75.5% in the control group; there was a statistically significant difference between the two groups (P<0.05). There were statistically significant pre-/post-treatment differences in CD3, CD4 and CD4/CD8 in the treatment group (P<0.05). There were statistically significant post-treatment differences in T cell subgroup indices (CD3, CD4 and CD4/CD8) between the treatment and control groups (P<0.05). Conclusions Combined use of acupuncture and medicine is an effective way to treat ulcerative colitis. It can regulate the numbers of CD3 and CD4 and improve bodily immunologic function in the patients.
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Objectives To evaluate the function of peripheral blood T cell subsets in uremia patients and the influence of hemodialysis on them.Methods Expression of CD3,CD4 and CD8 antigen on peripheral blood T cells from 30 cases of hemodialysis and non-hemodialysis patients was evaluated by flow cytometry.Fifteen volunteers were also examined as normal controls.Results The expression of CD3,CD4,CD8 and CD4 +/CD8 + ratio in non-hemodialysis group(NHD) was significantly lower than that of control group(P
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Aim To explore the effects of Yupingfeng polysaccharide(YPF-P)on T cells of adjuvant arthritis(AA)in rats.Methods Freund's complete adjuvant was used to induce AA in rat.Secondary paw swelling of AA rats was measured with volumeter.Peripneral blood lymphocyte proliferation response induced by concanavalin A(ConA)was examined with MTT assay.The CD4+ and CD8+ T cell in peripheral blood were detected by flow cytometer.ELISA method was applied to determine the content of factor IFN-? and radio-immunity assay of factor IL-4.RT-PCR procedures were utilized to determine the expression of IFN-? mRNA.Results 80,160 mg?kg-1 YPF-P could singnificantly inhibit the secondary paw swelling of AA rats.The suppressesion of lymphocyte proliferation of peripheral blood T cell in AA rats was reversed.After treatment by YPF-P,the number of CD4+ cell and the ratio of CD4+/CD8+ markedly decreased.The level of IFN-? in YPF-P treating group was obviously decreased in comparison with that of model control group,while the level of IL-4 was increased.The mRNA expression of IFN-? was inhibited as well.Conclusion Yupingfeng polysaccharide can regulate the turbulence of periphery abnormal T cell subgroups of adjuvant arthritis rats and can recover Th1/Th2 balance to move towards Th1,which may be one of its mechanisms of inhibiting the secondary paw swelling.