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Objective:To investigate the correlation between TNFAIP3 gene polymorphism and osteoporotic fractures in the elderly and bone metabolism indexes.Methods:A total of 115 patients with senile osteoporotic fractures admitted to Peace Hospital Affiliated to Shanxi Changzhi Medical College from Jan. 2019 to Jun. 2021 were enrolled as the observation group, and 120 patients with senile osteoporotic fractures matched with gender, age and body mass index of the observation group were selected as the control group. The levels of blood calcium, blood phosphorus, alkaline phosphatase, 25-hydroxyvitamin D and other bone metabolism indexes of all subjects were recorded. The genotypes of rs10499194 and rs13207033 in TNFAIP3 gene were analyzed by capillary electrophoresis and fragment analysis (SNaPshot) . The mRNA relative expression level of TNFAIP3 gene in peripheral blood of all subjects was determined by quantitative real-time fluorescence quantitative polymerase chain reaction.Results:There were statistically significant differences in genotype distribution and gene frequency of RS10499194 and RS13207033 between the observation group and the control group ( P<0.05) . For rs10499194, CT genotype carriers had a significantly higher risk of fracture than wild-type CC genotype carriers [OR=3.253 (1.223-8.652) , P=0.014]. The dominant model was also statistically significant ( P=0.003) . For rs13207033, GA carriers had a significantly higher risk of fracture than wild-type GG carriers [OR=3.775 (1.192-11.952) , P= 0.016]. The dominant model was statistically significant ( P=0.009) . The blood calcium level in AA+GA group was significantly higher than that in GG group at rs13207033 site ( P=0.006) . The mRNA expression level of TNFAIP3 gene in the observation group was 1.41±0.09, which was significantly lower than that in the observation group (2.07±0.12, t=6.69, P<0.001) . TNFAIP3 mRNA expression level of rs10499194 site TT+CT group was 1.35±0.11, significantly lower than CC group 1.43±0.13 ( t=2.82, P=0.007) . Conclusion:The polymorphism of TN-FAIP3 gene is related to the occurrence of osteoporotic fractures and bone metabolism, and may affect the gene expression level.
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Aim To investigate the mechanism of high mobility group protein B1(HMGB1)and tumor necrosis factor α-induced protein-3(TNFAIP3)involved in cell proliferation in lupus nephritis(LN)patients and human mesangial cells(HMC).Methods Immunofluorescence and immunohistochemistry technique were employed to detect HMGB1,TNFAIP3 and IκBα expression levels in glomerular cells of type Ⅳ LN patients.BrdU incorporation technology was used to detect cell proliferation level in HMC after stimulated by recombinant HMGB1.TNFAIP3 and IκBα expression levels in HMC were detected after HMGB1 stimulation by Western blot.Results The expression levels of HMGB1 and TNFAIP3 were increased in LN patients,while IκBα was decreased.HMC proliferation levels increased significantly after HMGB1 stimulation.At the same time,30 minutes after HMGB1 stimulation,the expression level of TNFAIP3 was significantly increased(P<0.05),while IκBα decreased(P<0.05)and then p65 increased significantly(P<0.05),compared with control group.Conclusion HMGB1 and TNFAIP3 are probably involved in mesangial cell proliferation by activating of NF-κB signaling pathways in LN pathogenesis.
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Purpose To explore the prognostic value of deletion of the TNFAIP3 gene in natural killer/T-cell lymphoma,nasal type detected with fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasm (FICTION).Methods FICTION was performed to detect the abnormalities of TNFAIP3 gene in 109 cases of natural killer/T-cell lymphoma,nasal type.Results TNFAIP3 deletion was found in 25/79 detectable cases.The deletion of TNFAIP3 was positively correlated with the International Prognosis Index (IPI) (P =0.019).Conclusion Frequent deletion of TNFAIP3 was associated with IPI in natural killer/T-cell lymphoma,nasal type,suggesting the important prognostic value of TNFAIP3 in the natural killer/T-cell lymphoma,nasal type.
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Ubiquitin plays a vital role in both protein degradation and many kinds of cellular functions, such as DNA damage repair, cell cycle regulation, cell growth and immune system function.Ubiquitin modiifed enzyme zinc ifnger protein A20 is considered to be an important gateway for the regulation of immune and inlfammatory responses, which is a key negative regulator in NF-κB signaling pathway. Dendritic cell (DC) is a full-time antigen presenting cell that identifies inflammatory response or pathogenic microorganism by multiple receptors, and is a key moderator for immunity homeostasis. Researches in recent years showed that A20 plays an important role in regulating the function of DC, which may take part in the occurrence and development of inlfammatory bowel disease. In this article, the regulation of A20 in the immunoregulation of DC and its function on the pathogenesis of inlfammatory bowel disease were reviewed.
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Background and purpose:Nasopharyngeal carcinoma is sensitive with radiotherapy, and it is of great signiifcance to study the resistance mechanism. This study aimed to analyze the expression of TNFAIP3 in the radiotherapy of nasopharyngeal carcinoma, the difference between radiosensitivity and radioresistance, the relationship with the occurrence and development of radioresistance.Methods:Detection of TNFAIP3 mRNA expression was carried out by TNFAIP3 multi-point labeled DIG probein situ hybridization.Results:In radiosensitivity and radioresistance of nasopharyngeal carcinoma, the moderately and strongly positive TNFAIP3 mRNA expression rates were 15% and 45%, (χ2=5.274,P=0.021 6), there were statistical significance. In the radioresistat nasopharyngeal carcinoma, TNFAIP3 mRNA moderately and strongly positive expression was positively correlated with TNM stage. In the degree ofⅢ-Ⅳ tumor, it had moderately and strongly positive expression rate of 48.28%.In the degree ofⅠ-Ⅱtumor, it had moderately and strongly positive expression rate of 36.36% (χ2=33.240,P<0.005); The expression rates in distant metastasis and no distant metastasis were 81.81% and 31.03% (χ2=6.385,P=0.011 5), the difference had statistical signiifcance.Conclusion:TNFAIP3 has antitumor effect, and it is correlated with the occurrence and development of radioresistant in nasopharyngeal carcinoma.