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Objective:To investigate the preventive and therapeutic effects of compound wild chrysanthemum eye pad on blue light-induced alteration of meibomian gland function in mice and its mechanism.Methods:Sixty-four 15-week-old male C57BL/6J mice were divided into two groups of 32 mice each according to random numbers for the prevention test and the treatment test.The respective 32 mice in the prevention and treatment experiments were randomly divided into normal group, blue light group, solvent group and eye pad group according to random numbers, with eight mice in each group, respectively.In the prevention experiments, mice in each group were exposed to blue light at a wavelength of 460 nm and a light intensity of 2 000 lx for 6 hours per day for 15 consecutive days to establish a mouse model of meibomian gland function changes except for the normal group.The solvent group and the eye pad group were treated with the corresponding eye pad before and after the blue light exposure for 25 minutes daily for the 15 consecutive days.The blue light group was treated with blue light exposure only for 15 days, and the mice were photographed at the edge of the meibomian gland on day 15 to observe the function of the meibomian gland except for the normal group.In the treatment test, all groups of mice except the normal group were induced the altered function of the mouse meibomian gland by the above method.The solvent and eye pad groups were treated with corresponding eye pads for 25 minutes in the morning and afternoon of each day for 15 consecutive days after blue light exposure.The blue light group was kept in a standard environment for 15 days and the changes in meibomian gland function of mice were detected by meibomian gland photographs on day 15.Photography of the eyelid margin in vitro, oil red O staining, and hematoxylin-eosin staining were performed to observe the histologic changes in the meibomian glands of mice after the preventive and experimental treatment.The relative expression of interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) mRNA in mouse meibomian gland tissues was detected by real-time fluorescence quantitative PCR.The expression of nuclear factor-κB (NF-κB) and phosphorylation of NF-κB (p-NF-κB) proteins in mice meibomian gland tissues was detected by Western blot to assess the degree of amelioration of blue light-induced inflammation in mouse meibomian glands by the compound wild chrysanthemum eye pad.This study was conducted in accordance with the Statement of the Association for Research in Vision and Ophthalmology on the Use of Animals in Ophthalmology and Vision Research, and was approved by the Animal Ethics Committee of Xiamen University (No.XMULAC20220258). Results:Compared with the normal group, a gradually increased number of blocked meibomian gland openings, and a gradually decreased remaining area of lower meibomian gland, were observed in the mice after 15 days of blue light group, and all the differences were statistically different (all at P<0.05). In the prevention test, the number of obstructed opening in the eye pad group was 1.833±0.753, which was significantly less than 3.667±1.033 in the solvent group ( P<0.05). The relative remaining area of the lower lid meibomian gland in the eye pad group was 0.718±0.091, which was significantly greater than 0.624±0.130 in the solvent group ( P<0.05). Hematoxylin-eosin staining showed inflammatory cell infiltration in mouse meibomian gland in the blue light and solvent groups.There was no inflammatory cell infiltration in eye pad group, and the morphology of the acini was similar to that of the normal group.Oil red O staining showed that there was no significant lipid deposition in the groups.The relative expressions of IL-1β, IL-6, TNF-α, and IFN-γ mRNA were significantly lower, and the relative expressions of NF-κB and p-NF-κB proteins were significantly lower in the eye pad group than in the solvent group, showing statistically significant differences (all at P<0.05). In the treatment test, the number of obstructed openings in the eye pad group and solvent group was 4.333±1.211 and 4.833±1.722, respectively, and the relative remaining area of the lower meibomian gland was 0.572±0.151 and 0.588±0.154, respectively, showing no statistically significant differences (both at P>0.05). Hematoxylin-eosin staining showed inflammatory cell infiltration in mouse meibomian glands in the blue light and solvent groups, with a similar morphology of acini as in the normal group.There was no inflammatory cell infiltration in eye pad group.Oil red O staining showed that there was no significant lipid deposition in the groups.The relative expressions of IL-1β, IL-6, and IFN-γ mRNA were significantly lower and the relative expressions of NF-κB and p-NF-κB proteins were significantly lower in the eye pad group than in the solvent group (all at P<0.05). Conclusions:Compound wild chrysanthemum eye pad may have preventive and therapeutic effects on blue light-induced changes in meibomian gland function by reducing the inflammatory response of meibomian gland tissue through the inhibition of the NF-κB signaling pathway.
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Objective: To study the therapeutic effect of compound Chinese medicine preparation of wild chrysanthemum and rhizoma drynariae combined with ornidazole in the treatment of chronic periodontitis, to explore the curative effect of this method in the treatment of chronic periodontitis, and to calarify its mechanism. Methods: A total of 112 patients with chronic periodontitis were divided into control group, ornidazole group, Chinese medicine group, Chinese medicine combined with ornidazole group (combination group) according the order of clinic time; there were 28 cases in each group. After basic periodontal treatment of the affected tooth (supragingival scaling and subgingival scaling), the periodontal pockets of the patients in control group were washed with 3% H2O2 and 0. 9%NaCl injection by turns, once a week; the patients in ornidazole group were orally administered with ornidazole one piece at a time, twice a day; the patients in Chinese medicine group were injected with compound Chinese medicine preparation of wild chrysanthemum and rhizoma drynariae into the pocket to make the medicine overflow to the edge of the gingival margin, once a week; the patients in combination group were injected with compound Chinese medicine preparation into the periodontal pocket while orally administered with ornidazole. All patients in various groups were treated for 4 weeks. The periodontal indexes including modified plaque index (mPLI), modified sulcus bleeding index (mSBI), probing depth (PD), and attachment loss (AL) of the patients in various groups were observed before and after treatment; and the levels of tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) in the gingival crevicular fluid (PISF) of the patients in various groups were detected after one course of treatment. Results: There were no significant differences in the periodontal indexes and the levels of TNF-αand PGE2 in PISF of the patients before treatment between various groups (P>0. 05). Compared with control group, the level of PGE2 in PISF in Chinese medicine group was reduced (P0. 05); the mPLI, mSBI, PD and AL, the TNF-α level in PISF of the patients in combination group were significantly reduced (P<0. 05). Compared with Chinese medicine group, the mPLI, mSBI, PD and AL, the levels of TNF-α and PGE2 in PISF of the patients in combination group were significantly reduced (P<0. 05). Conclusion: The traditional compound Chinese medicine preparation of wild chrysanthemum and rhizoma drynariae combined with ornidazole has the best therapeutic effect in the chronic periodontitis, which can improve the periodontal index and reduce the levels of TNF-α and PGE2 in PISF of the patients.
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Objective:To observe the effect and the underlying mechanism of wild chrysanthemum eye drops in the treatment of rabbit evaporative dry eye. Methods:The dry eye model was established by cauterizing the meibomian gland orifices in sixteen New Zealand white rabbits. In the sixth week after the operation, they were divided into four groups treated by wild chrysanthemum eye drops, dextran and hypromellose eye drops, solvent control solution and physiological saline, respectively. Corneal fluorescence stai-ning, rose bengal staining and conjunctival goblet cell density were performed and detected before and after the operation. Results:Be-fore the treatment, no significant difference was found in the tested indices among the four groups (P>0. 05). After the treatment, the fluorescence staining and rose bengal staining in wild chrysanthemum eye drops group were lighter than those in the untreated groups, and the conjunctival goblet cell density was higher than that in the untreated groups(P0.05). Conclusion: Wild chrysanthemum eye drops can alleviate ocular surface inflammation of dry eye in rabbits, which suggests good application prospect in the treatment of dry eye.
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Objective To evaluate the efficacy and safety of Prostant in the treatment of chronic prostatitis. Methods A randomized, double-blind, positive drug-controlled clinical trial method was applied in the study. Through clinical symptoms, signs and expressed prostate secretion (EPS) examination, 80 patients with prostatitis were recruited and randomized into a trial group (60 cases) and a control group (20 cases). The trial group was treated with Prostant, and the control group was treated with wild chrysanthemum suppository. After screening, treatment and follow-up, in the trial group and the control group, the observation on clinical efficacy and safety were evaluated, such as the clinical symptoms, signs, EPS examination, the national institute health-chronic prostatits symptom index (NIH-CPSI) and the blood routine, urine routine, stool routine, the function of liver and kindney, electrocardiograph examination and the side effects. Results After 4-week treatment, the total effective rate was 74.14% in the trial group and 40.0% in the control group, the efficacy of Prostant was superior to that of wild chrysanthemum suppository (P =0.006). There was significant difference in clinical symptoms and NIH-CPSI between pre-treatment and post-treatment in the trial group and the control group (P
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Objective:To approach the in unce of wild chrysanthemum on some neuroendoerine factors in ventricular remodeling induced by abdominal aortic banding(AAB)in rats.Methods:Myocardial hypertrophy,ventricular remodeling model was induced by abdominal aortic banding(AAB)in rats.After 35 days' treatment,the systolic blood pressure(SBP),diastolic blood pressure(DBP) and mean arterial pressure(MAP) were measured.Then the ratios of LVW/BW(1eft ventricle weight/ body weight),HW/BW(heart weight/body weight)were Calculated.The Angiotensin II(Ang II) content in heart tissue and the Aldosterone(ALD),Tumor Necrosis Factor(TNF-?)concentration of blood plasma were determined by radioimmunoassay.Results:The experimental data demonstrated that wild chrysanthemum could decrease SBP,DBP,MAP and the cardiac indexes of LVW/BW and HW/BW,significantly reduce the content of Ang II,ALD and TNF-?,decrease the Hydroxyproline content(P
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OBJECTIVE:To establish a HPLC method for content determination of luteolin wild chrysanthemum flower.METHODS:The separation was performed on a Kromasil C18 column,the mobile phase was composed of tetrahydrofuran-methanol-1.5%acetic acid(27∶4∶69),the flow rate was 1.0 ml/min,the ultraviolet wavelength was 353nm,the column temperature was 30℃.RESULTS:The calibration curve showed good linearity in the range of 0.028?g～0.56?g(r=1.0 000),the average recovery was 99.9%(RSD=2.28%).CONCLUSION:The method is simple,convenient and accurate and can be used for the quality control of wild chrysanthemum flower.