Individual Identification of Cartilage by Direct Amplification in Mass Disasters / 法医学杂志

OBJECTIVES@#To explore the effectiveness of direct amplification for the STR analysis of cartilage, and to accelerate the effectiveness of disaster victim identification.@*METHODS@#Eighty-eight cartilage samples were directly amplified by PowerPle® 21 kit, and the results of genotyping were compared with that obtained by the magnetic beads method.@*RESULTS@#In 88 cartilage samples, the STR genotypes were successfully detected from 84 samples by direct amplification and magnetic beads method, and both the results of genotyping by two method were consistent.@*CONCLUSIONS@#Direct amplification with PowerPlex® 21 kit can be used for STR genotyping of cartilages. This method is operated easily and promptly, which has a potential application in the individual identification of mass disasters.

Identification of Panax ginsheng C. A. Meyer Cv. Silvatica and cultivated Panax ginseng by DALP fingerprint / 中国药学杂志

OBJECTIVE: To explore the characteristics of DNA direct amplification of length polymorphisms (DALP) from wild ginsengs under forest and cultivated Panax ginseng. METHODS: The CTAB method was modified to extract genomic DNA from wild ginsengs under forest and cultivated Panax ginseng. Six random primers were designed, and DALP system was optimized for PCR amplification. RESULTS: Genomic DNAs of 19Kb were extracted by CTAB method from all the samples, and their purity was calculated to be (1.80±0.23). DALP fingerprints of wild ginsengs under forest and cultivated Panax ginseng were obtained. CONCLUSION: The DALP fringerprint of wild ginsengs under forest has a specific characteristics, and can be used to identify wild ginsengs under forest and cultivated Panax ginseng.

Establishment of DALP reaction system for plants of Rhodiola L / 中草药

Objective Direct amplification of length polymorphism (DALP) as a new molecular marker was used to establish a set of stable DALP reaction system for the plants of Rhodiola L. Methods Some significant parameters of DALP reaction procedure were investigated and optimized by taking the DNA genome for the plants of Rhodiola L. as template. Results The reaction system was : 20 ?L reaction system containing 2. 5 mmol/L Mg2+ , 1. 25 mmol/L dNTPs, 60 ng DNA template, 1 ?L 5 pmol/L selective primer, 3 ?L 5 pmol/L reverse primer, selective primer: reverse primer is 1 : 3, and 2 U Taq DNA polymerase. Amplification program is 95℃ pre-denatured for 5 min, 94℃ denatured for 30 s, 50℃ annealed for 30 s, 72℃ extending for 1 min; after 30 cycles, and then 72℃ extending again for 10 min to the end of PCR reaction. Conclusion This DALP reaction system is efficient to identify the species and local populations for the plants of Rhodiola L. repeatedly with the stronger stability and reliability.