RESUMEN
By means of the dialysis-type method of molecular species hybridization the individual differ ences of polypeptide chains of globin of SMMC/B (abbreviated as B) mice at the filial generations of F28 and F34, and at the same parental generation Bp (?) and Bp ( + ) as well as F35 (Bf) were investigated. The results of hybridization between mouse Hb and sheep Hb are as follows: All the hybrid groups exhibited four zones on polyacrylamide gel electrophoretogram, but all the control groups only two zones. The comparison of electrophoretograms showed that the electrophoretic positions of the new components were the same, indicating ? and ? chains assayed were quite close to each other, and no significant differences among individual and intergenerations were observed in B mice globins. By electrophoretogram analysis of the genetic expression products, we might distinguish preliminarily the genetic variation of ?- and ?-genes.
RESUMEN
The separation and purification of beta chains of globm from the inbreeding SMMC/B mice were performed by the dissociation of chains in urea, CMhcellulose column chromatography for the separation of alpha and beta chains, and Sephadex G25 column chromatography for removing salts. The pure beta-chain was digested by trypsin. Digested fragments were resolved by cellulose membrane two-way electrophoresis, thus obtaining the map of spots of beta-chain fragments for B33, B34, Bp(), Bp and Bf. The spots,BTp3, which have the same positions ,were separated and micro-sequences were assayed by DABITC/PITC double coupling technique. According to the genetic code, we might deduce the nucleotide sequence of their mRNA from the known amino acid sequence of BTp3, a piece of beta-chain. By analyzing the altered genetic loci of beta-chain of globin, we might determine the genetic purity of the strain and distinguish objectively the genetic types between species and subspecies.