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ObjectiveTo study the effect of a microbial compound fertilizer on the growth， quality， and soil properties of Andrographis paniculata and provide a basis for its application in the cultivation and production of A. paniculata. MethodThrough five treatment tests in pots， the control group （CK， no fertilization）， the treatment A group （chemical fertilization）， the treatment B group （microbial fertilization at 2.5 g·kg-1 soil）， the treatment C group （microbial fertilization at 7.5 g·kg-1 soil）， and the treatment D group （microbial fertilization at 12.5 g·kg-1 soil） were established. The effects of a compound microbial fertilizer on the agronomic properties， the content of active ingredients， the number of rhizosphere soil bacteria， Actinomycetes， and fungi， and soil physicochemical properties of A. paniculata were studied. ResultAs compared with the CK group and the treatment A group， the plant height， leaf number， leaf area， and above-ground and below-ground fresh weight of A. paniculata were significantly increased after microbial fertilization， with differences in different growth periods. The effect of the compound microbial fertilizer on the content of andrographolide， dehydrated andrographolide， neandrographolide， and 14-deoxyandrographolide differed. The content of andrographolide significantly increased by 26.13% and 13.23% in the treatment C group， respectively， as compared with the CK group and the treatment A group （P<0.05）. The content of neandrographolide increased with the increasing amount of microbial fertilizer， and the content in the treatment D group increased by 9.06% and 50.33%， respectively， as compared with the CK group and the treatment A group （P<0.05）， which was the most obvious. The content of 14-deoxyandrographolide in the treatment B group significantly increased by 42.04% and 1.74%， respectively， as compared with the treatment A group and the CK group （P<0.05）. The content of dehydrated andrographolide content in the treatment B group increased most significantly， significantly increasing by 11.73% and 27.74%， respectively， as compared with the CK group and the treatment A group （P<0.05）. The soil pH of the treatment B， C， and D groups was higher than that of the CK group and the treatment A group. The content of total nitrogen and effective phosphorus increased most significantly in the treatment B group， and the content of fast-acting potassium increased most significantly in the treatment D group. The application of the compound microbial fertilizer increased the number of rhizosphere soil bacteria and Actinomycetes， which was significantly higher than that in the CK group and the treatment A group （P<0.05）， and increased with the increase of applied amount. The number of fungi decreased with the increase of the compound microbial fertilizer application， which was significantly different from that in the CK group （P<0.05）. The fungi/bacteria of all treatment groups were significantly lower than those of the CK group （P<0.05） and increased with the increase of microbial fertilizer application. ConclusionThe application of the compound microbial fertilizer transforms the plant-soil-microbial system in a benign direction， which is beneficial to the growth of A. paniculata and the accumulation of active ingredients.
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ObjectiveTo explore the effects of three kinds of microbial fertilizers on the growth， yield， quality， and cadmium （Cd） accumulation of Chuanxiong Rhizoma （CX）. MethodTaking CX seeds as materials， field experiments were carried out in the main producing areas， Pengzhou and Meishan. The samples were collected during the harvesting period， and the agronomic characters and yield were determined. The contents of extract， volatile oil， and ferulic acid were analyzed by the collection method of Chinese Pharmacopoeia （2020 edition）. The content of Cd was determined by inductively coupled plasma-mass spectrometry （ICP-MS）. Data were processed by difference significance analysis， correlation analysis and cluster analysis. ResultThree kinds of microbial fertilizers with appropriate concentrations could promote the growth of CX. In terms of yield and quality， the treatment of Jinwuzong （1.50 ton/hm2， 1 ton=1 000 kg， the same below） and Cuijingyuan （1.5 L·hm-2） could increase the yield of medicinal materials by 0.92%-46.34%， while Cuijingyuan （1.8 L·hm-2） and Shenchu （15， 30 kg·hm-2） could increase the water-soluble extract of CX by 0.06%-18.79%， of which Cuijingyuan （1.8 L·hm-2） was significantly increased （P<0.01）. The alcohol-soluble extract of CX treated with Jinwuzong （0.75， 1.50， 2.25 ton/hm2）， Shenchu （15， 45 kg·hm-2）， and Cuijingyuan （1.2 L·hm-2） decreased significantly by 3.51%-22.94% （P<0.01）. The content of ferulic acid in CX treated with Jinwuzong （1.50 ton/hm2） and Shenchu （30 kg·hm-2） decreased by 2.14%-30.56%. Three kinds of microbial fertilizers had little effect on the content of volatile oil in CX. In the aspect of Cd enrichment， the concentration of Cd in rhizosphere soil of CX was increased by 11.33%-76.36% （P<0.01） after the treatment of Jinwuzong （0.75， 1.50， 2.25 ton/hm2）， Shenchu （15， 30， 45 kg·hm-2） and Cuijingyuan （1.2 L·hm-2）. However， the Cd enrichment coefficient of CX reduced by 2.58%-48.38%， the Cd content and Cd accumulation of CX decreased respectively by 9.54%-25.96% and 9.34%-18.88% via Jinwuzong （0.75 ton/hm2） and Cuijingyuan （1.8 L·hm-2）. ConclusionThree kinds of microbial fertilizers have a certain positive effect on the growth， substance accumulation， and reduction of Cd content in medicinal parts of CX， and the changes of each index are affected by the producing area and treatment method. Based on the comprehensive analysis of various indicators， Jinwuzong （0.75， 1.50 ton/hm2） can better adapt to the rhizosphere soil micro-ecological environment of CX， it can effectively reduce the content of Cd on the premise of guaranteeing the yield and quality of CX.
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The wild resources of Paris polyphylla var. yunnanensis, a secondary endangered medicinal plant, are severely scarce. Introduction and cultivation can alleviate market demand. To screen phosphatolytic bacteria in the rhizosphere soil of P. polyphylla var. yunnanensis and provide data support for the development of high-efficiency microbial fertilizer, in this study, the dilution plate coating method was used to isolate and screen the phosphorus solubilizing bacteria with the ability of mineralizing organic phosphorus from the rhizosphere soil of wild and transplanted varieties of P. polyphylla var. yunnanensis in 10 different locations in Yunnan, Sichuan and Guizhou. After separation and purification, the phosphatolytic capacity was analyzed by qualitative and quantitative analysis. Combined with physiological and biochemical experiments, the strains were identified using 16 S rDNA sequencing analysis. Forty one strains were selected from the rhizosphere soil of P. polyphylla var. yunnanensis from 10 different habitats. Among them, 21 strains were obtained from the rhizosphere soil of the wild variety P. polyphylla var. yunnanensis and 20 strains were obtained from the rhizosphere soil of the transplanted variety. And significance analysis found that 41 organophosphate solubilizing strains had significant differences in their ability to solubilize phosphorus. The amount of phosphate solubilizing was 0.08-67.61 mg·L~(-1), the pH value was between 4.27 and 6.82. The phosphatolytic amount of strain Y3-5 was 67.61 mg·L~(-1), and the phosphorus increase amount was 57.57 mg·L~(-1). All 41 strains were identified as Gram-positive Bacillus. Combining physiological characteristic and phylogenetic trees, Bacillus mobilis Y3-5 was finally selected as the candidate rhizosphere phosphatolytic bacteria of P. polyphylla var. yunnanensis. The distribution of phosphorus solubilizing bacteria in the rhizosphere soil of P. polyphylla var. yunnanensis was different, and there were significant diffe-rences in phosphorus solubility. Organophosphate-dissolving strain Y3-5 is expected to be a candidate strain of P. polyphylla var. yunnanensis microbial fertilizer.
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Bacillus , Bacterias/genética , China , Liliaceae , FilogeniaRESUMEN
BACKGROUND: There is a large amount of industrial wastewater produced by the mushroom industry during the canning processing each year, which could provide abundant carbon, nitrogen and inorganic salts for microbial growth. The aim of this study was to optimize the culture conditions for Bacillus licheniformis cultured in the Agaricus bisporus industrial wastewater to produce the agricultural microbial fertilizer. RESULTS: In this work, the maximal biomass of B. licheniformis could be obtained under the following culture conditions: 33.7°C, pH 7.0, 221 rpm shaking speed, 0.5% wastewater, 2 (v:v, %) inoculum dose, loading liquid of 60 mL/250 mL and a culture time of 24 h, and the average experimental value obtained was 1.35 ± 0.04 × 109 Obj/mL, which was within the 95% confidence interval of the predicted model (1.291.38 × 109 Obj/mL), and met the national microbial fertilizers' standard in China. Furthermore, the field experiment results showed that the fermentation broth of B. licheniformis could significantly improve the yield of Anoectochilus roxburghii. CONCLUSIONS: Agaricus bisporus industrial wastewater can be used to produce agricultural microbial fertilizer.