1.
International Journal of Traditional Chinese Medicine
;
(6): 806-808, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-437230
RESUMEN
Objective To establish a HPLC method for the determination of berberine hydrochloride in Jiawei Ermiao Wan.Methods The RP-HPLC system consisted of the Luna-C18 (4.6 mm × 250 mm,5 μm) column,the mobile phase consisted of acetonitrile-0.1%H3PO4(45:55),the flow rate was 1.0mL · min-1,the column temperature was 30℃,The UV detector was set at 245nm.Results The linear response range was 0.071~1.42 mg· ml-1 (r=0.9998).The average recovery of mangiferin was 99.2%,and RSD<2%.Conclusion The method was simple,rapid,accurate and repeatable.It could be applied in the determination of berberine hydrochloride in Jiawei Ermiao Wan.