Mechanism of Liangfu Dropping Pills in treatment of gastrointestinal diseases based on plasma pharmacochemistry and network pharmacology / 中国中药杂志

To study the active chemical components and mechanism of Liangfu Dropping Pills in treatment of gastrointestinal diseases. The UHPLC-Q-TOF-MS method was employed to analyze the components of Liangfu Dropping Pills in plasma. The protein targets of the absorbed compounds were predicted in the TCMSP database and the SwissTargetPrediction database. The targets associated with gastrointestinal diseases were collected from OMIM, CTD, GeneCards, and DrugBank. The common target genes between components and diseases were screened out for the building of protein-protein interaction(PPI) network in the STRING database. Metascape was used to carry out gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis. Cytoscape was employed to construct the PPI network diagram and absorbed component-target network diagram. The molecular docking between the components absorbed in blood and potential key targets was performed by AutoDock vina 4.2.6 to screen and verify the main active components and targets. Twelve chemcial components were identified in Liangfu Dropping Pills, in which four components were absorbed in blood, including galangin, rhamnocitrin, galangin 3-methyl ether, and α-cyperone. These components acted on 189 common targets which were mainly involved in the cell responses to nitrogen compounds, organic cyclic compounds, and hormones, and enriched in the PI3 K-Akt signaling pathway, Foxo signaling pathway, and IL-17 signaling pathway. Molecular docking results showed that the four components had strong affinity with core targets. The material basis of Liangfu Dropping Pills treating gastrointestinal diseases may be galangin, rhamnocitrin, galangin 3-methyl ether, and α-cyperone. This study provides a theoretical basis for further development and application of Liangfu Dripping Pills.

Studies on plasma fingerprint of Danggui Shaoyao Powder by UPLC-UV / 中草药

Objective To establish the plasma fingerprint of Danggui Shaoyao Powder (DSP) for the analysis of the transitional components in rat plasma after administration of DSP extracts Methods Eleven batches of rat plasma were prepared after oral administration and the plasma fingerprint was established by UPLC-UV. The transitional components in rat plasma after administration of DSP extracts was analyzed by UPLC-Q/TOF-MS. Results The plasma fingerprints of common peaks for 11 batches of DSP were established to ascertain the optimized blood collection time, and the method was used to process the plasma. Fifteen common peaks were detected in plasma fingerprint, and the similarity were both higher than 0.933. The methodology of plasma pharmacochemistry was adopted to analyze the common peaks, and 15 transitional components, including 10 prototype components and five metabolites were identified. Conclusion The established plasma fingerprint of DSP provide the basis for the further study of transitional components.