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AIM:To study the effect of diallyl trisulfide (DATS) on experimental corneal neovascularization (CNV) in rats induced by corneal suture and detect the expression of vascular endothelial growth factor (VEGF) and p-AKT in rats cornea.METHODS:The rat model of corneal neovascularization (CNV) was induced by corneal suture.Rats were randomly divided into Group A:physiological saline control group containing DMSO (10 rats);Group B:25μmol/L DATS treatment group (10 rats);Group C:50μmol/L DATS treatment group (10 rats);Group D:100μmol/L DATS treatment group (10 rats);Group E:200μmol/L DATS treatment group (10 rats).The occurrence and development of CNV were observed by slit-lamp microscope at 7d after suture,and the area of CNV were calculated.Two weeks later,HE staining was used to observe the pathological organization form of each cornea,and RT-PCR and Western blot were used to detect the expression of VEGF mRNA and protein expression of VEGF and p-AKT between each groups.RESULTS:The blood vessel area of Group C,D and E was compared with that of Group A,the difference was statistically significant (P＜0.05);HE slice showed corneal edema,angiogenesis and inflammation infiltration situation gradually reduced comparing with the Group A,with the increase of concentration of DATS.RT-PCR showed the expression of VEGF mRNA in Group B,C,D,and E decreased compared with the Group A,and the difference was statistically significant (P＜0.05).Western-blot showed that the expressions of VEGF and p-AKT in Group B,C,D and E decreased gradually compared with those in Group A,and the difference was statistically significant (P＜0.05).CONCLUSION:DATS can inhibit corneal neovascularization of the rats induced by suture.Its mechanism may be associated with suppression of VEGF secretion,down-regulation of VEGF and inactivation of p-AKT.
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Objective: Blunt and penetrating injuries to the anus and rectum are uncommon. Considerable debate remains regarding the optimal treatment of anorectal injuries. This report offers a technique for repair of anorectal wound from under its floor. Design: The anorectal wound was repaired by multiple double incomplete circular stitches under its floor. Setting: This study was conducted in the General Surgery Department, Zagazig University Hospital, Egypt. Patients: This study was undertaken on 20 patients (15 male and 5 female with mean age of 40.7 years) with anorectal wounds from April 2012 to May 2014. Interventions: Under general or spinal anesthesia, primary repair of anorectal wounds by special stitches passed under the wound floor not crossing the wound edges, without fecal diversion and presacral drainage. Results: In 12 patients, the technique was done under general anesthesia and the remainder under spinal anesthesia. The anorectal wounds were healed within 8 weeks. Early complications included superficial wound infection in 15 patients, urine retention in 9 patients, and transient incontinence in 18 patients for period ranged from 4 to 6 weeks. No permanent incontinence, no deep infection, no abscess or fistular formation, no keyhole deformity, and no mortality were recorded. Conclusion: Our technique for primary repair of anal wound and sphincter is simple, effective, and no need for fecal diversion or presacral drainage.
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A new radical operational procedure designed by ourselves has been used on 40 cases of congenital megacolon in our department during 1977-1991, The result of the follow-up sur- vey of 35 cases was gained. The operative curative rate was 97.1% and mortality was 2.9%. The characteristic is that the method of extraction by around-anastomosis stitches was used. Several important factors which affected operative effects were discussed. Four radical operational methods were compared. Our results demonstrate that the new method is effec- tive, safe, practical and feasible particularly in Yunnan. This study provides an ideal operational method in the clinical treatment of congenital megacolon.