1.
Indian J Exp Biol
;
2001 Sep; 39(9): 947-9
Artículo
en Inglés
| IMSEAR
| ID: sea-62624
RESUMEN
A protocol was developed for high frequency plant regeneration in H. patulum by shoot-tip culture. H. patulum plants were collected from a wild source growing at high altitude in the eastern Himalayas. Multiple buds were initiated from shoot-tips cultured on Murashige and Skoog's medium supplemented with BAP, kinetin. Addition of thiamin HCI, Ca-pantothenate and biotin enhanced multiple shoot formation. Upon transfer to phytohormone free liquid medium following a brief exposure to auxin, root formation occurred from the micro shoots . Rooted plants were hardened and transferred to soil. Regeneration potentiality was found to be constant throughout the year in long term cultures.
Asunto(s)
Adenina/análogos & derivados , Biotina/metabolismo , Medios de Cultivo , Hypericum/fisiología , Ácidos Indolacéticos/farmacología , Cinetina , Ácido Pantoténico/metabolismo , Reguladores del Crecimiento de las Plantas/farmacología , Raíces de Plantas/crecimiento & desarrollo , Brotes de la Planta/crecimiento & desarrollo , Plantas Medicinales/fisiología , Purinas/farmacología , Regeneración/fisiología , Tiamina/metabolismo
2.
Indian J Exp Biol
;
1973 Mar; 11(2): 114-6
Artículo
en Inglés
| IMSEAR
| ID: sea-58147