Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats

To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy. METHODS: Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long- laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed. RESULTS: G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4. CONCLUSION: The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections.

Resultats des recherches de l'etude fluorometrique des medicaments homeopathiques / Research's results of fluorimetric assay with homeopathic medicines

Determinou-se o espectro fluorimetrico das altas dinamizacoes dos medicamentos homeopaticos que ja tinham sido estudados em 2DH e 2CH. Observou-se que, quando as dinamizacoes sao baixas, o lugar da fluorescencia varia de uma dinamizacao a outra, talvez devido a presenca da grande variedade de compostos que ainda se encontram presentes nessas potencias. Parecia que a partir da 6CH as dinamizacoes se "incorporassem" ao medicamento, sendo minima a variacao da regiao da fluorescencia, dando lugar entao a uma notavel variacao da intensidade das curvas, ja que ainda na 12CH e 30CH seguem fluorescendo, apesar de encontrar-se alem do numero de Avogadro. E tambem muito interessante que a intensidade relativa das curvas, aumente a medida que aumenta o numero de agitacoes, chegando ao seu ponto maximo de intensidade nas 100 sucussoes