Isolation and identification of endophytic fungi from Chelidonium majus and their antifungal activity / 中国中药杂志

In order to find new source of antifungal agents, eleven cultivable endophytic fungi were isolated from the roots,stems and leaves of Chelidonium majus by traditional method. Seven of them were identified as Colletotrichum(L1, L2, L3, S1, S3, S4, S5), and three of them were identified as Fusarium(R1,R2,R3) by morphological features and molecular biological technology. The antifungal activity test showed that all the tested fungi displayed some inhibitory activity against five common plant pathogens(C. gloeosporioides, Curvularia lunata, Pyricularia oryza, Alternaria alternate and A. brassicae), and their inhibition rate of some test items were over 60%. Among them, R1, S2, S3 and S4 were more potent than others. This study enriches the understanding of endophytes from Ch. majus and provides a basis for the study of new microbial fungicides.

Evaluation of pro-apoptotic activity of Chelidonium majus L. and/or gamma-radiation against ehrlich as carcinoma transplanted in mice / Avaliação da atividade pró-apoptótica da Chelidonium majus L. e/ou radiação gama contra o tumor ascítico de ehrlich transplantado em ratos

This study was to evaluate, the pro-apoptotic activity of ethanolic Chelidonium majus L. (CM) leaf extract and/or gamma-radiation in Ehrlich ascites carcinoma (EAC) bearing mice by measuring tumor volume, apoptotic factors (caspase-3, Bcl-2 and Bax proteins, tumor necrosis factor-alpha (TNF-), angiogenesis factors (matrix metalloproteinase (MMP-2 and MMP-9); tissue inhibitor of metalloproteinases (TIMP-1), DNA fragmentation, besides histopathological examination. After tumor inoculation, mice received CM (100 mg/body weight/day) on the 10th day and were exposed to whole body -radiation (6Gy) on the 17th day. The data obtained reveales that combining CM with - radiation exposure induce significant regression in tumor growth, up-regulate Bax, caspase-3, TIMP-1 and inhibit Bcl-2, TNF-, MMP-(2 and 9). Cytotoxicity is substantiated by increased DNA-fragmentation (comet assay and DNA content) and histological examination. It could be suggested that combining CM with gamma-radiation increased apoptosis of tumor cells which would help to increase the lifespan of mice.

Este estudo foi realizado para avaliar a atividade pró-apoptótica do extrato etanólico da folha da Chelidonium majus L. (CM) e/ou radiação gama no tumor ascítico de Ehrlich tendo ratos para a medição do volume do tumor, fatores apoptóticos (caspase-3, proteínas Bcl-2 e Bax, fator de necrose tumoral (TNF-)), fatores angiogênicos (metaloproteinase matriz (MMP-2 e MMP-9)); inibidor tecidual de metaloproteinase (TIMP-1), fragmentação de DNA, além de exame histopatológico. Depois da inoculação do tumor, os ratos receberam CM 100 mg/peso corporal/dia no 10º dia e tiverem o corpo completo exposto a radiação gama (6Gy) no 17º dia. Os dados obtidos revelam que combinar CM com exposição à radiação gama induz a uma regressão significativa no crescimento do tumor, regula positivamente Bax, caspase-3, TIMP-1 e inibe Bcl-2, TNF-, MMP-(2 e 9). Citotoxicidade é substanciada pelo aumento da fragmentação do DNA (ensaio cometa e conteúdo de DNA) e exame histopatológico. Poderia ser sugerido que combinar CM com radiação gama aumentou a apoptose das células tumorais o que ajudaria a aumentar a expectativa de vida dos ratos.

Chelidonium majus-Induced Acute Hepatitis / 이화의대지

The use of traditional folk remedies is increasing throughout Asia. Chelidonium majus, a popular herbal remedy, is used to treat abdominal pain caused by various gastrointestinal disorders, including gastric ulcer, gastritis, and biliary tract disease, because of its morphine-like effect. We encountered a 62-year-old woman with acute hepatitis, in which C. majus was suspected to be the etiological factor. The patient had taken high dose of C. majus extract for the preceding 60 days. The clinical context and the temporal association between the start of the herbal medicine treatment and her liver injury allowed us to attribute a causative role to C. majus. The diagnosis was confirmed by liver biopsy and the Council for International Organizations of Medical Sciences/Roussel Uclaf Causality Assessment Method (CIOMS/RUCAM) scale. After C. majus was discontinued, the liver function was restored to normal. In conclusion, because the use of phytotherapy is increasing, we wish to raise awareness of the potential adverse effects of C. majus.

Screen for natural benzylisoquinoline alkaloids against tumor / 中国中药杂志

<p><b>OBJECTIVE</b>To search for natural benzylisoquinoline alkaloids against tumor.</p><p><b>METHOD</b>In this study, taken DNA as target, a method was developed for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine by the use of centrifugal ultrafiltration combined with HPLC technology. The anti-tumor activity for the compounds screened was evaluated in vitro.</p><p><b>RESULT</b>Nine compounds interacted with DNA were discovered and identified from Macleaya cordata, Chelidonium majus, Coptis chinensis, and the proliferation of four types of human solid cancer cell lines was markedly inhibited by these compounds.</p><p><b>CONCLUSION</b>The developed method was considered to be suitable for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine.</p>

Determination of chelerythrine in Chelidonium majus by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus.</p><p><b>METHOD</b>Chelerythrine was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine (25:75) (adjusted pH to 3 using phosphoric acid) and the detection wavelength was set at 268 nm.</p><p><b>RESULT</b>The linear range of calibration curve was 0.051 6-0.516 0 microg (r = 1.000). The average recovery (n = 6) was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns.</p><p><b>CONCLUSION</b>The method is accurate, sensitive and reliable.</p>

Dyeing of cotton fabrics using some natural dyes part II: some studies on dyeing properties of cotton fabrics using chelidonium majus [Roots]

The pre-treated cotton fabric with tannic acid [60g/l] was dyed in a bath containing natural colouring matter extracted from vegetable plants Chelidonium majus [roots]. Factors affecting dyeing properties such as concentration of colouring matter as well as pH values, time and temperature of dyeing bath were studied. It was found that the most suitable dyeing properties are obtained when colouring matter is obtained from 18 g Chelidonium majus [roots]/100 ml water using 5g/l sodium chloride, L.R 1:50, pH 6, dyeing was continued for 30 min at 70°C. Environmentally friendly mordants such as copper sulphate, ferrous sulphate, stannous chloride and alum were added using three mordanting methods: [pre-simultaneous - post] mordanting methods and overall fastness properties of dyed mordanted fabrics were assessed. In the recent years, growing interest in the revival of natural dyes has been manifested. This interest is the result of a worldwide movement to protect the environment for indiscriminate exploitation and pollution by industries. Natural dyes are considered to be complementary and not conflictive with the use of manufactured dyes in the textile industry as a whole. Chelidonium majus is an erect plant from 2-4 feet and is native to temperate Europe and Asia. Leaves are deeply pinnatifid, crenate or lobed, flowers are yellow medium sized, in a small peduncled umbel, sepals 2, petals 4; stamens many ovary of 2 carpels, fruit is a cylindrical capsule 1-2 inch long. It belongs to papaveraceae family[1]. The present work was carried out to optimize the conditions for dyeing cotton with natural colouring matter derived from Chelidonium majus [roots]. The evaluation of [K/S, L, a, b, delta E] for different dyed mordanted and/or dyed washed fabrics. Estimation of metal ions [ppm] of the dyed mordanted cotton samples [pre-treated with 60 g/l tannic acid] was carried out[9-2]

Studies of some Egyptian medicinal plants as natural wealth in regression of hepatocellular carcinoma [HCC]

The present investigation was designed to valuate the antiangiogenic efficacy of some local medicinal plants against liver cancer induced by diethylnitrosamine [DEN] methanolic extracts of cupressus sempervirens, chelidonium majus and tropaeolum majus were used in this study. Vascular endothelial growth factor [VEGF], alpha-Feto-protein [AFP], ferritin, alanine transaminase [ALT], aspartate transaminase [AST], Alkaline phosphatase [ALP], superoxide dismutase [SOD] and Glutathione peroxidase [GPx] were determined in blood while Nitric oxide [NO] was determined in liver tissue homogenate. Furthermore histopathological investigations ere done. The results showed that the treatment with methanolic extracts of all selected plants led to a significant reduction in the level of all the desired biochemical parameters, while the administration of cupressus sempervirens only improved the histopathological pictures of liver architecture. These results suggest a beneficial effect of these plant extracts against experimentally-induced hepatocellular carcinoma and the possible mechanism of the protective effects may be partly due to the antioxidant activity of these plants

Evaluation of protective potentials of a potentized homeopathic drug, Chelidonium majus, during azo dye induced hepatocarcinogenesis in mice.

Several cytogenetical and enzymatic protocols were used to test if two microdoses of Chelidonium majus, namely Chelidonium-30 (Ch-30) and Chelidonium-200 (Ch-200), used as homeopathic drugs, showed anti-tumor activity and also favorably modulated genotoxic damages produced by an azo dye in mice at several intervals of fixation. Different sets of healthy mice were fed: (i) hepatocarcinogen, p-dimethylaminoazobenzene (p-DAB, initiator) + phenobarbital (PB, promoter), (ii) only p-DAB, (iii) only PB, and (iv) neither p-DAB nor PB (normal control). Mice fed with p-DAB + PB were divided into different sets that were also fed either Ch-30 (v) or Ch-200 (vi) or diluted alcohol (vii), the "vehicle" of the microdoses of Chelidonium. All mice of group (i), a few of group (ii) and group (vii) and none of groups (iii) and (iv) developed tumors in liver at the longer intervals of fixation. The frequencies of chromosome aberrations (CA), micronucleated erythrocytes (MN), mitotic index (MI) and sperm head abnormality (SHA) were much higher in groups (i) and (vii) mice than in groups (ii), (iii) and (iv) mice at all fixation intervals. However, in mice of both groups (v) and (vi), the frequencies of CA, MN, SHA were strikingly less than those of groups (i) and (vii), and moderately less than those of groups (ii) and (iii). Both Ch-30 and Ch-200 also modulated favourably some toxicity marker enzymes like acid and alkaline phosphatases, peroxidases, glutamate oxaloacetate and glutamate pyruvate transaminases in liver, kidney and spleen tissues of the carcinogen fed mice. The microdoses of Chelidonium having no visible ill effects of their own, may be strong candidates for use in delaying/protecting liver cancer.

Chelidonium majus var asiatica / 대한간학회지

No abstract availalbe.