Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
1.
Braz. j. med. biol. res ; 51(5): e6213, 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-889085

RESUMEN

Dermatophagoides farinae (Der f), one of the main species of house dust mites, produces more than 30 allergens. A recently identified allergen belonging to the alpha-tubulin protein family, Der f 33, has not been characterized in detail. In this study, we used bioinformatics tools to construct the secondary and tertiary structures and predict the B and T cell epitopes of Der f 33. First, protein attribution, protein patterns, and physicochemical properties were predicted. Then, a reasonable tertiary structure was constructed by homology modeling. In addition, six B cell epitopes (amino acid positions 34-45, 63-67, 103-108, 224-230, 308-316, and 365-377) and four T cell epitopes (positions 178-186, 241-249, 335-343, and 402-410) were predicted. These results established a theoretical basis for further studies and eventual epitope-based vaccine design against Der f 33.


Asunto(s)
Animales , Tubulina (Proteína)/química , Alérgenos/química , Epítopos de Linfocito T/química , Epítopos de Linfocito B/química , Dermatophagoides farinae/química , Antígenos Dermatofagoides/química , Tubulina (Proteína)/genética , Tubulina (Proteína)/inmunología , Alérgenos/genética , Alérgenos/inmunología , Estructura Molecular , Estructura Terciaria de Proteína , Mapeo Epitopo , Epítopos de Linfocito T/genética , Epítopos de Linfocito B/genética , Biología Computacional , Análisis de Secuencia de Proteína , Dermatophagoides farinae/genética , Dermatophagoides farinae/inmunología , Antígenos Dermatofagoides/genética , Antígenos Dermatofagoides/inmunología
2.
The Korean Journal of Parasitology ; : 239-243, 2007.
Artículo en Inglés | WPRIM | ID: wpr-219735

RESUMEN

Many allergists are currently focusing on the development of new diagnostic tools, and are attempting to improve both the sensitivity and specificity. A multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) is one of the most popular diagnostic tools used in the Republic of Korea. However, there remains controversy among allergists with regard to the cut-off point for a positive result. The present study was conducted in order to determine the validity of MAST-CLA as compared with that of the skin prick test, with particular emphasis on arthropod allergens, on the basis of percentage agreement rates and k-values, and also to suggest the optimal positive cutoff points using receiver operating characteristic (ROC) curves. The study was conducted with 97 subjects (54 men, 43 women). Optimal individual cut-off points were calculated as follows; class II for Dermatophagoides farinae, class I for Dermatophagoides pteronyssinus, and trace for a cockroach mix. These findings suggest that attempting to apply optimal individual cut-off points will be a good way of improving diagnostic tests, particularly MAST-CLA.


Asunto(s)
Adulto , Animales , Femenino , Humanos , Masculino , Alérgenos/inmunología , Antígenos Dermatofagoides/inmunología , Mediciones Luminiscentes/métodos , Cucarachas/química , Dermatophagoides farinae/química , Dermatophagoides pteronyssinus/química , Hipersensibilidad/diagnóstico , Proteínas de Insectos/inmunología , Curva ROC , Pruebas Cutáneas/métodos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA