1.
Experimental & Molecular Medicine
;
: 240-245, 1998.
Artículo
en Inglés
| WPRIM
| ID: wpr-159763
RESUMEN
We studied the regulation of fibronectin (FN) gene expression by cAMP and phorbol-12-myristate-13-acetate (PMA) in HT-1080 human fibrosarcoma cells. Dibutyryl cAMP increased FN synthesis and mRNA levels, while PMA inhibited the cAMP-induced FN synthesis. In transient transfection assays, cAMP increased FN promoter activity, while PMA paradoxically enhanced the cAMP-induced promoter activity. Stable transfection experiments, however, showed that neither cAMP or PMA alone nor together affected FN promoter activity. These results suggest that PMA antagonizes the cAMP-induced FN gene expression and that both the action of cAMP and the inhibition of its action by PMA may occur at the posttranscriptional level in HT-1080 cells.
Asunto(s)
Humanos , Northern Blotting , Bucladesina/farmacología , Bucladesina/antagonistas & inhibidores , Ensayo de Inmunoadsorción Enzimática , Fibronectinas/metabolismo , Fibronectinas/genética , Fibrosarcoma/genética , Regulación de la Expresión Génica , Luciferasas/metabolismo , Pruebas de Precipitina , Regiones Promotoras Genéticas , ARN Mensajero/metabolismo , Acetato de Tetradecanoilforbol/farmacología , Transfección , Células Tumorales Cultivadas , beta-Galactosidasa/metabolismo
2.
Indian J Cancer
;
1967 Sep; 4(3): 298-303
Artículo
en Inglés
| IMSEAR
| ID: sea-50895