Investigation of the histopathological level of Ki-67, caspase-3 expressions of the effects of hesperidin on wound healing in the rat esophagus

Purpose: The effects of hesperidin application on the wound caused by esophageal burns were investigated in this study. Methods: Wistar albino rats were divided into three groups: Control group: only 1 mL of 0.09% NaCl was administered i.p. for 28 days; Burn group: An alkaline esophageal burn model was created with 0.2 mL of 25% NaOH orally by gavage­1 mL of 0.09% NaCl was administered i.p. for 28 days; Burn+Hesperidin group: 1 mL of 50 mL/kg of hesperidin was given i.p. for 28 days to rats after burn injury. Blood samples were collected for biochemical analysis. Esophagus samples were processed for histochemical staining and immunohistochemistry. Results: Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were significantly increased in Burn group. Glutathione (GSH) content and histological scores of epithelialization, collagen formation, neovascularization was decreased. After hesperidin treatment, these values were significantly improved in the Burn+Hesperidin group. In the Burn group, epithelial cells and muscular layers were degenerated. Hesperidin treatment restored these pathologies in Burn+Hesperidin group. Ki-67 and caspase-3 expressions were mainly negative in control group; however, the expression was increased in the Burn group. In the Burn+Hesperidin group, Ki-67 and caspase-3 immune activities were reduced. Conclusion: Hesperidin dosage and application methods can be developed as an alternative treatment for burn healing and treatment.

Experiencia clínica con un flavonoide purificado en el tratamiento de cefaleas resistentes a otras terapias / Clinical experience with a purificated flavonoid in the headache treatment resistent to other therapy

Las cefaleas de tipo vascular (migrañas) siempre han sido un problema difícil de tratar, en el presente trabajo reportamos un hallazgo muy esperanzador con el uso de un Flavonoide Purificado (Diosmina-Hesperidina, que utilizamos con gran éxito en las insuficiencias venosas), para el tratamiento de esas cefales en el transcurso de seis (6) meses hicimos el seguimiento de veintisiete (27) pacientes que poseían un largo historial de cefaleas resistentes a tratamientos específicos y tradicionales en estas afecciones, sin mejoría. Con una dosis de Flavonoide Purificado de 500 mgs V.O cada 12 horas tuvimos un 81,5 por ciento de beneficio en cuanto a remisión de las crisis y mejoría de las mismas. Creemos que este hallazgo merece reportarse pues resulta una novedosa esperanza terapéutica