Manifestaciones de la cavidad bucal por infección del virus de la inmunodeficiencia humana: reporte de caso clínico / Oral manifestations of the HIV virus: report of a clinical case

Las manifestaciones orales por la infección del virus de la inmunode-ficiencia humana son en ocasiones el primer signo de la enfermedad yen muchos casos un indicador de la progresión de la infección hacia elsíndrome de inmunodeficiencia adquirida. Las ulceraciones indoloras,diferentes tipos de gingivitis agresivas y la leucoplasia vellosa, se desarrollan muy fácilmente en individuos cuyo sistema inmunológico está comprometido, como el de los pacientes que sufren del virus deinmunodeficiencia adquirida.

Oral manifestations caused by the human immunodefi ciency virusare often the fi rst indication that the person is infected and oftenan indicator of its progression into AIDS. Painless ulcers, assortedtypes of aggressive gingivitis, and hairy leukoplakia develop easilyin individuals whose immune system is compromised, such as thoseinfected with HIV.

Oral manifestations and their correlation to baseline CD4 count of HIV/AIDS patients in Ghana

OBJECTIVES: Acquired immunodeficiency syndrome (AIDS) is a disease of the human immune system caused by the human immunodeficiency virus (HIV). People with AIDS are much more vulnerable to infections, including opportunistic infections and tumors, than people with a healthy immune system. The objective of this study was to correlate oral lesions associated with HIV/AIDS and immunosuppression levels by measuring clusters of differentiation 4 (CD4) cell counts among patients living in the middle western regions of Ghana. MATERIALS AND METHODS: A total of 120 patients who visited the HIV clinic at the Komfo Anokye Teaching Hospital and the Regional Hospital Sunyani of Ghana were consecutively enrolled in this prospective and cross-sectional study. Referred patients' baseline CD4 counts were obtained from medical records and each patient received an initial physician assessment. Intraoral diagnoses were based on the classification and diagnostic criteria of the EEC Clearinghouse, 1993. After the initial assessment, extra- and intraoral tissues from each enrolled patient were examined. Data analyses were carried out using simple proportions, frequencies and chi-square tests of significance. RESULTS: Our study included 120 patients, and was comprised of 42 (35.0%) males and 78 (65.0%) females, ranging in age from 21 to 67 years with sex-specific mean ages of 39.31 years (males) and 39.28 years (females). Patient CD4 count values ranged from 3 to 985 cells/mL with a mean baseline CD4 count of 291.29 cells/mL for males and 325.92 cells/mL for females. The mean baseline CD4 count for the entire sample was 313.80 cells/mL. Of the 120 patients we examined, 99 (82.5%) were observed to have at least one HIV-associated intraoral lesion while 21 (17.5%) had no intraoral lesions. Oral candidiasis, periodontitis, melanotic hyperpigmentation, gingivitis and xerostomia were the most common oral lesions. CONCLUSION: From a total of nine oral lesions, six lesions that included oral candidiasis, periodontitis, melanotic hyperpigmentation, gingivitis, xerostomia and oral hairy leukoplakia were significantly correlated with declining CD4 counts.

Oral lesions associated with human immunodeficiency virus in 75 adult patients: a clinical study

OBJECTIVES: The objective of this study was to investigate the presence of oral lesions in human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) patients in a descriptive cross-sectional study, and to establish their presence according to levels of CD4+ cells (including the CD4+/CD8+ cell ratio). MATERIALS AND METHODS: A total of 75 patients infected with HIV were included. Oral lesions were observed and classified using World Health Organization classification guidelines. Potential correlations between the presence and severity of oral lesions and CD4+ cells, including the CD4+/CD8+ cell ratio, were studied. RESULTS: The most frequent oral lesion detected was oral pseudomembranous candidiasis (80.0%), followed by periodontal disease (40.0%), herpetic lesions (16.0%), hairy leukoplakia (16.0%), gingivitis (20.0%), oral ulceration (12.0%), Kaposi's sarcoma (8.0%), and non-Hodgkin's lymphoma (4.0%). The CD4+ count was 500 cells/mm³ in 12 cases (16.0%). The mean CD4+ count was 182.18 cells/mm³. The mean ratio of CD4+/CD8+ cells was 0.26. All patients showed at least one oral manifestation. CONCLUSION: There was no correlation between the CD4+/CD8+ cell ratio and the presence of oral lesions. The severity of the lesions was more pronounced when the CD4+ cell count was less than 200 cells/mm³.

Retrospective analysis of the clinical behavior of oral hairy leukoplakia in 215 HIV-seropositive patients

Abstract Oral manifestations are common findings in human immunodeficiency virus (HIV) infected patients and frequently influence the overall health. Oral hairy leukoplakia (OHL) is strongly associated with HIV infection demonstrating its relationship with the individual's immune status and progression of immunosuppression. This study aims to retrospectively evaluate OHL in HIV patients, analyzing its incidence, demographic aspects and possible changes in clinical and epidemiological profile of the disease over 17 years. The records of 1600 HIV-infected patients were reviewed. The data were correlated and analyzed, considering HIV exposure category, age, gender, harmful habits, CD4 level, use and type of antiretroviral. OHL was observed in 215 (13.4%) patients. Most were men in the fourth decade of life, 171 (79.5%) and 112 (52,1%) respectively, but an increase in the incidence of OHL among female patients and those in the fifth decade of life was observed. Tobacco smoking was the most frequent harmful habit reported by 114 (68%) patients. OHL occurred mostly in patients with CD4 counts between 200 and 500 cells/mm3 35 (55.5%). The lower incidence of OHL was found among patients using at least one non-nucleoside reverse transcriptase inhibitor (NNRTI). OHL is related to CD4 count, use of ARVT and tobacco smoking and is also more prevalent in men in the fourth decade of life. These characteristics were recognized in absolute values, but when verifying the behavior over the years we noticed that the incidence of OHL is decreasing and its epidemiological characteristics changing.

A Case of Secondary Syphilis Presenting as a "Leukoplakia-like" Plaque on the Tongue in a HIV Patient / 대한피부과학회지

"Leukoplakia-like" plaque on the tongue is an uncommon skin manifestation of secondary syphilis. Skin lesions of secondary syphilis usually have numerous presentations, which mimic many other skin diseases, especially in the presence of HIV co-infection. Oral hairy leukoplakia (OHL) is characterized by corrugated whitish patches and plaques on the lateral border of the tongue. It is frequently and strongly associated with HIV but may appear in other diseases. A 47-year-old man with HIV and receiving HAART therapy developed a leukoplakia-like plaque on the tongue, which was first suggestive of OHL but was eventually diagnosed as secondary syphilis.

Aspectos demográficos e manifestações clínicas bucais de pacientes soropositivos para o HIV/Aids / Demographic and clinical manifestations of oral in patients seropositive for HIV /SIDA

Introdução: As lesões bucais e peribucais são comuns nos pacientes infectados pelo vírus HIV e podem representar os primeiros sinais da doença, antes mesmo das manifestações sistêmicas. Objetivo: Este estudo objetivou estimar a prevalência de manifestações bucais em indivíduos soropositivos para o HIV, considerando aspectos sociodemográficos, imunológicos e terapêuticos. Material e Método: Foi realizado um estudo de natureza clínico-epidemiológica transversal, entre outubro de 2007 e abril de 2008, com amostra composta por 40 pacientes. Utilizou-se a técnica de observação direta intensiva por meio de exame clínico apropriado. Os dados foram registrados em fichas pré-elaboradas e analisados por estatística descritiva e inferencial. Resultado: Verificou-se a prevalência do gênero feminino (52,5%), na faixa etária de 40 a 49 anos (45%). O tratamento com antirretrovirais foi constatado em 85,0% dos casos, sendo todos com terapia de alta potência (HAART). Observou-se que 52,5% dos pacientes apresentaram a contagem de linfócitos TCD4+ acima de 500cél/mm³ e 50,0% apresentaram carga viral indetectável. A prevalência das manifestações bucais foi 42,5%, sendo a mais expressiva a candidose pseudomembranosa (19,23%), seguida da periodontite úlcero-necrosante (15,38%), da leucoplasia pilosa (11,54%) e da queilite angular (11,54%). Não foi verificada associação entre manifestações bucais e carga viral (p=0,1268), nem com o número de células T CD4 (p=0,3458). Conclusão: A prevalência de algumas manifestações bucais associadas à infecção pelo HIV ainda é alta, sendo a candidose pseudomembranosa a infecção mais prevalente, principalmente entre pessoas com baixo nível ...

Introduction: The oral and perioral lesions are common in patients infected with HIV, and may represent the first signs of the disease, even before the systemic manifestations. Objective : This study aimed to estimate the prevalence of oral manifestations in HIV seropositive individuals, considering the aspects socio-demographic, immunological and therapeutic. Material and Method: Was performed nature study clinical-epidemiological transverse, between October 2007 to April 2008, with a sample of 40 patients. Was used the technique of intensive direct observation through appropriate clinical exam. Data were recorded on data sheets pre-prepared and analyzed by descriptive and inferential statistics. Result: There was a prevalence of females (52.5%), aged 40-49 years (45%). Treatment with antiretroviral drugs was found in 85.0% of cases, all being with high power therapy (HAART). It was observed that 52.5% of patients had a TCD4+ lymphocyte count above 500cél/mm ³ and 50.0% had an undetectable viral load. The prevalence of oral manifestations was 42.5%, the most significant for pseudomembranous candidiasis (19.23%), followed necrotizing ulcerative periodontitis (15.38%), hairy leukoplakia (11.54%) and angular cheilitis (11.54%). No association was observed between oral lesions and viral load (p=0.1268) nor the TCD4 cell count (p=0.3458). Conclusion: Prevalence of some oral manifestations associated with HIV infection is still high, with pseudomembranous candidiasis infection more prevalent, mainly among people with low education levels and longer HIV infection, independent of the therapy and immune status of the patient. .

Clinical features of oral lesions in patients with human immunodeficiency virus infection/acquired immunodeficiency syndrome in Guangxi autonomous region / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the features of oral lesions in patients with human immunodeficiency virus infection/acquired immunodeficiency syndrome (HIV/AIDS).</p><p><b>METHODS</b>A total of 127 HIV-seropositive patients were interviewed for health information and examined for their HIV-related oral lesions according to the EC Clearing House Criteria on Oral Problems related to HIV-Infection (1992). The examinations were conducted by dental specialist and HIV specialist. The CD4 T cell count in peripheral blood of the patients was tested by flow cytometry. The patients were divided into HIV- infected group (42) and AIDS group (85) according to CDC Classification System for HIV- Infected Adults and Adolescents (revised in 1993). Chi-square test was used to test the relationship between systemic disease and oral lesions, and the difference of the prevalence of oral lesions between the two groups.</p><p><b>RESULTS</b>Among the 127 patients, oral candidiasis (51/127), oral hairy leukoplakia (24/127) were common oral manifestation. There was no relationship between the oral manifestation and systemic disease (P = 0.397). The occurrence of oral lesions and oral candidiasis was significantly different between the two groups (χ² = 7.684, P = 0.006; χ² = 14.410, P < 0.001). The CD4 count was related to the prevalence of oral lesions (P = 0.006) and oral candidasis (P = 0.003).</p><p><b>CONCLUSIONS</b>Most oral lesions appeared before the appearance of systemic disease. Oral candidiasis and oral hairy leukoplakia were the most common lesions.Oral lesions had no relationship with systemic disease but could be still an indicator for disease progress.</p>

Comparison of oral manifestations with CD4 count in HIV-infected patients.

Aim and Objective: This study was carried out with the primary aim of correlating oral changes and general changes of HIV-infected patients with their CD4 count. Materials and Methods: 124 patients were selected, and after taking their informed consent, they were subjected to detailed history taking and thorough clinical examination. Specific oral lesions and general physical changes were recorded. Every patient was subjected to laboratory investigation for CD4 count. All these findings were tabulated. The clinical observation and laboratory findings were subjected to critical analysis and correlated. Statistical test, i.e. Student's " t" test, was applied and objective conclusions were drawn. Result: Out of 124 patients, 40 had oral candidiasis, 6 had oral hairy leukoplakia, 12 had periodontal disease, 20 had xerostomia, 30 had melanin pigmentation, while 4 had HSV2, and atypical ulceration. Out of 40 patients with oral candidiasis, 28 patients had CD4 count <200 (group A), 10 patients were in group, B (CD4 count 200-500 cell/mm 3 ) and 2 patients in group C(CD4 >500 cell/mm 3 ). Oral hairy leukoplakia occurred in equal proportions in group A and B. These periodontal diseases were more commonly in group B; xerostomia and melanin pigmentation was equally seen in group A and B. Conclusion: Oral candidiasis, oral hairy leukoplakia, linear gingival erythema, necrotizing ulcerative gingivitis, and necrotizing ulcerative periodontitis are specific oral indicators which will definitely suggest to the dental surgeon that the disease is running a rapid downhill course and due to this the oral physician is in a position to raise a suspicion and alert the general physician regarding the declining immune status of patient.

Incidence of oral hairy leukoplakia in human immunodeficiency virus-seropositive adult patients in Yunnan, China / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the incidence rates, clinical characteristics of oral hairy leukoplakia (OHL) and its relation to the immune status in a sample of human immunodeficiency virus (HIV)-infected adults in Yunan, China.</p><p><b>METHODS</b>1 060 adult patients with HIV from January 2008 to June 2010 were evaluated. The age, gender, education grade, diagnosis time of HIV-infected, route of transmission, xerostomia, oral candidiasis, high active antiretroviral therapy and CD4 lymphocytes counts. The occurrence of OHL was recorded by oral examination. The relationship of CD4 lymphocytes counts and the incidence of OHL were analyzed by statistical methods.</p><p><b>RESULTS</b>There were 94 OHL patients in 1 060 HIV patients (8.9%). The average age of the OHL patients was (39.33 +/- 10.45) years old. 90% OHL was found on the two lateral aspect of the tongue. The CD4 lymphocytes of 70.2% OHL patients were less than 200 mm-3.</p><p><b>CONCLUSION</b>OHL is a frequent finding in patients with indicates severe immunosuppression and associated with the reduction of CD4 lymphocytes.</p>

Human papillomavirus and Epstein Barr virus in oral hairy leukoplakia among HIV positive Venezuelan patients

Oral hairy leukoplakia (OHL) is commonly found in individuals infected with HIV, and represents the most frequent oral manifestation. The purpose of this study was to detect the presence of Human Papillomavirus (HPV) and Epstein Barr Virus (EBV) in OHL of HIV+ Venezuelan patients. We evaluated 21 HIV+ adult patients with clinically present OHL lesions: 11 under antiretroviral therapy, 10 without therapy, and 10 oral mucosal samples as controls. Nested-PCR was used to detect EBV and HPV infection. The INNO-LiPA HPV Genotyping v2 was applied to determine the HPV genotype. The EBV genome was found in 16/21 (76%) of the HIV+ patients with OHL. No difference was observed in EBV+ and EBV- patients related to antiretroviral therapy viral load and CD4+ T cell count. HPV-DNA was observed in 7/21 HIV positive cases (33%). The HPV genotypes detected were: 6, 11, 31, 33, 52, and 56/74. The most frequently HPV found was genotype 6 in 7/7, while two cases were HPV-11 and two HPV-52. Of the positive cases, 5/7 (71%) presented co-infection with more than one HPV genotype and 4/7 (57%) had HPV coinfection with high and low risk types. No case was EBV or HPV positive in the control group. In this study, a higher EBV prevalence was observed in OHL-HIV+ patients, confirming the etiologic role in this entity. A considerable number of cases were positive for HPV infection, and many patients presented coinfection with more than one HPV genotype as well as the presence of high oncogenic risk HPV in OHL.

El proposito del presente estudio fue detectar la presencia de virus papiloma humano (VPH) y Epstein Barr (VEB) en Leucoplasia Vellosa Oral (LVO) de pacientes VIH positivos. Se evaluaron 21 pacientes adultos VIH positivos con lesiones clinicas presentes de LVO y 10 casos controles de mucosa sana. Para el diagnostico molecular de VPH y EBV se utilizo Nested PCR. La determinacion de los genotipos se realizo mediante el kit HPV INNO-LiPA genotyping v2. La presencia de genoma de VEB se demostro en un alto porcentaje (76%) en 16/21 de los pacientes VIH positivos con LVO. No se observo relacion entre los pacientes VEB+ y VEBcon el uso de terapia antirretroviral, la carga viral y el contaje de celulas T CD4+. Se demostro la presencia de ADN-VPH en 7/21 (8%) de los casos VIH positivos. Los genotipos de VPH detectados fueron 6, 11, 31, 33, 52, 56/74. El genotipo 6 fue el mas frecuentemente observado en 7/7, dos casos fueron VPH 11 y dos VPH 52. De los casos positivos 5/7 (71%) presentaron coinfeccion con mas de un genotipo de VPH y en 4/7 (57%) se evidencio coinfeccion con tipos de alto y bajo riesgo oncogenico. En el presente estudio se observo una alta prevalencia de VEB en pacientes VIH positivos con LVO, confirmando el papel etiologico en esta entidad. Un considerable numero de casos fueron positivos para VPH. Se observo la presencia de coinfeccion con mas de un tipo viral, asi como la presencia de VPH de alto riesgo.

Tratamento da leucoplasia pilosa: revisão da literatura / Treatment of oral hairy leukoplakia: a literature review

Introdução: Pacientes com imunosupressão pela infecção do HIV (Human Immunodefi ciency Vírus) freqüentemente desenvolvem lesões de boca como a leucoplasia pilosa (LP). LP é uma lesão quase que exclusivamente observada em indivíduos infectados pelo HIV que apresentam uma baixa contagem de linfócitos T CD4. LP é assintomática e aparece como placa branca na borda lateral da língua, de superfície plana, corrugada ou pilosa e não removível quando raspada. A etiologia da LP é relacionada ao vírus Epstein-Barr, que pode ser identificado através de técnicas de microscopia eletrônica, hibridização "in situ", imunoistoquímica e reação em cadeia da polimerase. A citologia esfoliativa é uma boa opção para o diagnóstico da LP, por ser um método simples, confiável, seguro, não invasivo e não traumático. O tratamento da LP é recomendado para eliminar as pilosidades, restaurar o conforto do paciente, re-estabelecer as características normais da língua e eliminar nichos de bactérias, vírus e fungos desencadeadores de outras doenças da boca. Os tratamentos propostos na literatura para LP incluem cirurgia, terapia anti viral sistêmica e a terapia tópica. Objetivo: Realizar uma revisão de literatura sobre as opções de tratamento para a LP. Conclusão: A terapia tópica tem sido a mais recomendada, por ser de fácil aplicação, pouco invasiva, de baixo custo, com poucos efeitos colaterais e, principalmente, para eliminar as pilosidades e EBV, para restabelecer as características normais da língua sem desenvolvimento de resistência aos vírus e para eliminar nichos bacterianos e fúngicos. Indicações clínicas para esse tratamento devem incluir sintomas associados à lesão ou um desejo do paciente, por razões estéticas de eliminar a lesão.

Introduction: Patients with immunosupression caused by the human immunodeficiency virus (HIV) infection frequently develop oral lesions such as oral hairy leukoplakia (OHL). OHL is a lesion almost exclusively observed in HIV-infected individuals, who present low T CD4 lymphocyte count. OHL is asymptomatic and appears as a white plaque on the lateral border of the tongue, with fl at, corrugated or hairy surface, which is not detachable when scraped. The etiology of OHL is related to Epstein-Barr virus, which may be detected by electron microscopy, in situ hybridization, immunohistochemistry, and polymerase chain reaction techniques. Exfoliative cytology is a good option for the diagnosis of OHL, for being a simple, reliable, safe, noninvasive and non-traumatic method. The treatment of OHL is recommended to eliminate the filiform structures on lesion surface, provide comfort to the patient, re-establish the normal characteristics of the tongue and eliminate possible niches for bacteria, viruses and fungi that may cause other oral diseases. The treatments proposed in the literature for OHL include surgery, anti viral systemic therapy and topical therapy. Objective: This paper presents a literature review about the treatment options for OHL. Conclusion: Topical therapy has been the most recommended because it is easy to apply, is a low-invasive and low-cost procedure, and has few side effects. It is mainly indicated for removing the filiform structures on lesion surface, eradicating the Epstein-Barr virus, reestablishing the normal characteristics of the tongue without developing resistance to the virus, and eliminating bacterial and fungal niches. Clinical indications for this treatment should include symptoms associated with the lesion or the patient's desire of eliminating the lesion for esthetic reasons.

Oral Hairy Leukoplakia Which Occurred as a Presenting Sign of Acute Myeloid Leukemia in a Child

Oral hairy leukoplakia (OHL) is caused by the reactivation of a previous Epstein-Barr virus (EBV) infection in the epithelium of the tongue. Most lesions are characterized by corrugated whitish patches on the lateral border of the tongue. It is frequently associated with AIDS, but cases in patients with other immunosuppressed states have also been reported. In leukemia patients, OHL is rarely encountered, and appears only after chemotherapy. We report a case of OHL which occurred as a presenting sign of acute myeloid leukemia (AML) in a previously healthy 15-year-old child. A 15-year-old boy presented with a whitish patch on the left lateral border of the tongue. The biopsy specimen revealed papillomatosis, hyperkeratosis, acanthosis and ballooning degeneration in the stratum spinosum. The patient was EBV seropositive, and PCR analysis of EBV DNA in the lesional tissue was positive. After the diagnosis of OHL in dermatologic department, the patient was referred to pediatrics due to the abnormal peripheral blood smear, and was diagnosed with AML.

Detección de Candida Albicans en lesiones de leucoplasia vellosa bucal de un grupo de pacientes venezolanos VIH+ / Detection of candida albicans in lesions of oral hairy leukoplakia a group of HIV + patients in Venezuela

El objetivo del presente estudio fue determinar la prevalencia de la infección por Candida albicans en lesiones de Leucoplasia Vellosa Bucal (LVB) en un grupo de pacientes VIH+ en una muestra de la población venezolana. Para ello se evaluaron 21 pacientes adultos VIH+, con lesiones clínicas de Leucoplasia Vellosa Bucal, 11 con terapia antiretroviral y 10 sin terapia, y 10 pacientes adultos VIH- con lesiones hiperqueratósicas de la mucosa oral provenientes del Centro para la Atención de Pacientes con Enfermedades Infectocontagiosas de la Facultad de Odontología de la Universidad Central de Venezuela. Los pacientes fueron examinados clinícamente para la detección de lesiones presentes en la mucosa oral, confirmándose el diagnóstico con el estudio histopatológico. La infección por C albicans se determinó mediante biopsias de las lesiones para su estudio histopatológico empleando dos técnicas diferentes, coloración de Pass y método de Grocott, y cultivo microbiológico en medio Agar Sabouraud Resultados: En pacientes VIH+ según los distintos métodos empleados se pudo observar que mediante el cultivo en Agar Sabouraud 6/21 (29%) de los pacientes fueron positivos y 15/21 (71%) fueron negativos. Con respecto a la coloración de Grocott, todos los 21 pacientes fueron positivos, mientras que con la coloración de PAS, 17/21 (81%) de los pacientes fueron positivos y 4/21 (19%) fueron negativos. En el caso de los pacientes VIH- con leucoplasia bucal, 1/10 (10%) resultó positivo mediante el cultivo en Agar Sabouraud y 9/10 (90%) fueron negativos. Mediante la coloración de Grocott todos los pacientes (10) fueron negativos para la presencia del hongo, e iguales resultados fueron obtenidos con la coloración de PAS. Conclusión: Se observó una mayor prevalencia de la infección por C. albicans en pacientes VIH+ lo que demuestra que la presencia del hongo, constituye un factor fuertemente asociado a los pacientes con LVB

The aim of this study was to determine the prevalence of Candida albicans infection in Oral Hairy Leukoplakia (OHL) in Venezuelan HIV+ population. We evaluated 21 HIV+ adults patients with the clinical diagnosis of OHL, 11 under antiretroviral therapy, 10 without and 10 HIV negative adults with hyperkeratotic lesions on the oral mucosa as a control group, from the Center of Infectious diseases, Faculty of Dentistry, Central University of Venezuela. All patients were clinically examined and definitive histopathological diagnosis was made. C albicans was detected by two different histochemical methods: PAS and Groccot and microbiological culture using Agar Sabouraud medium. The results demonstrated that 6/21 (29%) patients were positive by microbiological culture and 15/21 (71%) were negative. All the patients were positive by Grocott special stain while 17/21 (81%) were positive using PAS. In the control group, 1 patient was positive by culture and 9/10 (90%) were negative. Additionally, all the patients were negative by Grocott and PAS. In conclusion, we observed a higher prevalence of C. albicans infection in HIV+ patients, suggesting that the presence of this microorganism may constitute an associated factor in OHL

Prevalência da leucoplasia pilosa oral: identificação citopatológica das formas clínica e subclínica / Prevalence od oral hairy leukoplakia: cytopathologic identification of the clinical and subclinical forms

Introdução - Vários estudos apontam para a importância do diagnóstico da leucoplasia pilosa oral (OHL) no diagnóstico e prognóstico da AIDS. Raros estudos utilizam a citopatologia como recurso da OHL, particularmente após a descrição de sua forma subclínica. O objetivo deste trabalho foi investigar a prevalência da leucoplasia pilosa oral em indivíduos soropositivos para o HIV. material e métodos - Realizou-se a oroscopia e raspagem bilateral das bordas laterais da língua, em 676 pacientes HIV+. O critério diagnóstico foi a a identificação das alterações nucleares (inclusão Cowdry A e núcleos em vidro fosco e em colar) representativas do efeito citopático do Vírus epstein-Barr (EBV). Resultados - Identificou-se OHL em 179 (27 por cento) dos 676 pacientes, sendo em 65 (10 por cento) OHL clínica e em 114 (17 por cento) OHL subclínica...

Introduction - Accurate diagnosis of oral hayry leukoplakia (OHL) is important because it many be an early indicator of undiagnosed HIV infection and AIDS's prognostic. Few studies tested cytopathology as a method for diagnosis of OHL, particularly after the description of it subclinical stage. Our purpose was to investigated the lateral borders of tongues of 676 patients. OHLwas considered as a diagnosis only when nuclear changes caused by Epstein-Barr Virus (Cowdry tupe A inclusion, ground glass and nuclear beading) were presents. Results - We identified OHL in 179 (27 percent) of the 676 patients, that 65 (10 percent) were clinica OHL and 114 (17 percent) were OHL in subclinical stage...

Tratamento tópico da leucoplasia pilosa bucal e desenvolvimento e validação de um instrumento para avaliação do conhecimento de cirurgiões dentistas sobre HIV/AIDS / Topical treatment for oral hairy leukoplakia and development and construct validation of a questionnaire for assessment od dentists knowledge about HIV/AIDS

A tese apresentada constou de duas pesquisas. A primeira foi um estudo de ensaio clínico randomizado com os objetivos de apresentar um novo tratamento tópico para a leucoplasia pilosa bucal (LPB), utilizando-se solução alcoólica de podofilina a 25% associada ao penciclovir creme a 1% (PP); avaliar variáveis de prognóstico que podem influenciar nos tratamentos tópicos com solução alcoólica de podofilina a 25% (P), com solução alcoólica de podofilina a 25% ao aciclovir a 5% (PA) e com PP; e avaliar o desempenho desses três protocolos de tratamento tópico através da cura ou não da LPB, do índice de cura em relação ao tempo e da recidiva doze meses após a cura. As variáveis de prognóstico avaliadas foram: gênero, idade, anos de educação formal, rota de transmissão, CD4, CD8, carga viral, plaquetas, candidíase oral, uso de terapia antiretroviral altamente ativa (HAART), uso prévio de aciclovir, uso prévio de antifúngico, uso de drogas injetáveis, uso de AZT, consumo de cigarro e álcool. Realizou-se também a pesquisa da presença do vírus Esptein-Barr (EBV) antes e depois do tratamento tópico da LBP. Os 42 pacientes HIV-positivos e portadores de 69 lesões de LPB foram recrutados aleatoriamente do Centro de Treinamento e Referência em Doenças Infecciosas e Parasitárias Orestes Diniz (CTR-DIP), em Belo Horizonte, Minas Gerais. A presença do EBV foi avaliada em 15 destes 42 pacientes, onde a extração do DNA foi realizada por meio de raspado da borda lateral de língua e amplificada pela reação em cadeia de polimerase (PCR) antes e após o tratamento tópico. A LPB foi diagnosticada pela citologia esfoliativa. O modelo proporcional de Cox foi usado para avaliar as variáveis de prognóstico e o desempenho dos três protocolos de tratamento...

Morsicatio Labiorum/Linguarum: Three Cases Report and a Review of the Literature

Morsicatio is a condition caused by habitual chewing of the lips (labiorum), tongue (linguarum), or buccal mucosa (buccarum). Clinically, it often produces a shaggy white lesion caused by pieces of the oral mucosa torn free from the surface. The condition is generally found among people who are stressed or psychologically impaired. Most patients with this condition are not even aware of their biting habit. Clinically, morsicatio mimics hairy leukoplakia, and sometimes, it may be confused with other dermatologic diseases involving the oral cavity. It is rarely described in pathologic and dermatological textbooks. Histological features are distinctive, however, being careful to make a correct diagnosis can help one avoid providing inappropriate treatment. In this report we describe three cases of morsicatio, one that developed in the lower lip and the others that developed on the side of the tongue.

AIDS-associated Kaposi 's sarcoma on left lower retromolar triangle and parapharyngeal area : A case report

There are several oral lesions related with AIDS, such as candidiasis, hairy leukoplakia, Kaposi's sarcoma, aphthous stomatitis, lichen planus, and other opportunistic infectious diseases. Among the others, Kaposi's sarcoma, the most common malignant tumor associated with AIDS, is closely linked to the number of CD4+ T cell. Kaposi's sarcoma often occurs in palate, the most prone site, and has characteristic clinical features in most cases. Sometimes, the tumor induces underlying bone destruction at late stage. We report a case of a 27 year-old man with AIDS-associated Kaposi's sarcoma at left lower retromolar triangle, parapharyngeal area and discuss the management of AIDS patients in dentistry.

Epidemiological and oral manifestations of HIV-positive patients in a specialized service in Brazil

The purpose of this study was to evaluate the prevalence of oral lesions in HIV-positive patients attending the Specialized Service for Infectious-contagious Diseases and Parasitoses of the Health Secretariat of the State of Pará (URE-DIPE/SESPA), in the city of Belém, PA, Brazil. A total of 79 HIV-positive patients (53 males and 26 females) were examined. Clinical and epidemiological evaluations were done by correlating the lesions with gender, race, chronological age, risk behavior and prevailing immune status (CD4+ cells count). Lesion location and the presence of associated factors, such as alcohol use, smoking and denture wearing, were quantified individually for each type of lesion using a diagnostic pattern based on the clinical aspects. Approximately 47% of the patients (n=37) presented some type of oral lesion. Candidiasis (28%) and periodontal disease (28%) were the most common, followed by cervical-facial lymphadenopathy (17.5%). Other lesions observed were hairy leukoplakia, melanin hyperpigmentation, ulcerative stomatitis (aphthous), herpes simplex, frictional keratosis and pyogenic granuloma. This analysis presented some relevance as to the statistical data. Concerning CD4+ cells, most lesions manifested with the reduction of the CD count. There were a larger number of HIV-positive female heterosexual patients. Alcohol and/or smoking were strongly associated with the occurrence of hairy leukoplakia in these patients. Candidiasis and periodontal disease were the most common oro-regional clinical manifestations in the patients.

Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients

The Epstein-Barr virus (EBV) is the etiological agent of oral hairy leukoplakia (OHL), an oral lesion with important diagnostic and prognostic value in acquired immunodeficiency disease syndrome. The two EBV genotypes, EBV-1 and EBV-2, can be distinguished by divergent gene sequences encoding the EBNA-2, 3A, 3B, and 3C proteins. The purpose of this study was to identify the EBV genotype prevalent in 53 samples of scrapings from the lateral border of the tongue of HIV-1 seropositive patients, with and without OHL, and to correlate the genotypes with presence of clinical or subclinical OHL with the clinic data collected. EBV-1 and EBV-2 were identified through PCR and Nested-PCR based on sequence differences of the EBNA-2 gene. EBV-1 was identified in the 31 samples (15 without OHL, 7 with clinical OHL and 9 with subclinical OHL), EBV-2 in 12 samples (10 without OHL, 1 with clinical and 1 subclinical OHL), and a mixed infection in 10 samples (2 without OHL, 3 with clinical and 5 with subclinical OHL). The presence of EBV-1 was higher in women, but a significant statistical result relating one the EBV genotypes to the development of OHL was not found. We conclude that the oral epithelium in HIV-1 seropositive patients can be infected by EBV-1, EBV-2 or by a mixed viral population.

Prevalence of oral hairy leukoplakia and epithelial infection by Epstein-Barr virus in pregnant women and diabetes mellitus patients: cytopathologic and molecular study

Oral hairy leukoplakia (OHL) is generally reported in patients with severe immunosuppression, except for a few cases in individuals with moderate degree of immunodeficiency. It is a white lesion that appears mainly in the lateral border of the tongue, caused by Epstein-Barr virus (EBV). The nuclear changes caused by EBV (Cowdry A inclusion, ground glass and nuclear beading), observed in cytopathology, are specific and enough for the definitive diagnosis of OHL, independent of the identification of the virus. Here we investigated the prevalence of OHL and the presence of EBV-DNA in the lateral borders of the tongue from 90 pregnant women, 90 diabetes mellitus (DM) patients, 30 healthy individuals (negative group) and 30 HIV+ with OHL (positive group). Smears were analyzed by cytopathology and polymerase chain reaction (PCR). A case of subclinical OHL and candidiasis was identificated in a DM patient by cytopathologic analysis. PCR results demonstrated EBV-DNA in 65 percent of the pregnant women, in 35 percent of DM patients, and in 20 percent of the healthy individuals. We concluded that DM patients can develop OHL with a low prevalence. Furthermore, the prevalence of the EBV in lateral border of the tongue is larger in pregnant women than in healthy individuals.