Asunto(s)
Animales , Ratas , Factor Natriurético Atrial/metabolismo , Calcio/farmacología , Miocitos Cardíacos/efectos de los fármacos , Miocitos Cardíacos/enzimología , Poli(ADP-Ribosa) Polimerasas/metabolismo , Angiotensina II/farmacología , Animales Recién Nacidos , Factor Natriurético Atrial/genética , Calcio/metabolismo , Activación Enzimática/efectos de los fármacos , Quinasas MAP Reguladas por Señal Extracelular/metabolismo , /metabolismo , Regulación de la Expresión Génica/efectos de los fármacos , Espacio Intracelular/efectos de los fármacos , Espacio Intracelular/metabolismo , Contracción Miocárdica/efectos de los fármacos , Fosforilación/efectos de los fármacos , Regiones Promotoras Genéticas/genética , Unión Proteica/efectos de los fármacos , Proteínas Proto-Oncogénicas c-fos/genética , Proteínas Proto-Oncogénicas c-fos/metabolismo , Ratas Sprague-Dawley , Ribosa/metabolismoRESUMEN
The effects of black rice anthocyanidins (BRACs) on retinal damage induced by photochemical stress are not well known. In the present study, Sprague-Dawley rats were fed AIN-93M for 1 week, after which 80 rats were randomly divided into two groups and treated with (n = 40) or without BRACs (n = 40) for 15 days, respectively. After treatment, both groups were exposed to fluorescent light (3,000 +/- 200 lux; 25degrees C), and the protective effect of dietary BRACs were evaluated afterwards. Our results showed that dietary BRACs effectively prevented retinal photochemical damage and inhibited the retinal cells apoptosis induced by fluorescent light (p < 0.05). Moreover, dietary BRACs inhibited expression of AP-1 (c-fos/c-jun subunits), up-regulated NF-kappaB (p65) expression and phosphorylation of IkappaB-alpha, and decreased Caspase-1 expression (p < 0.05). These results suggest that BRACs improve retinal damage produced by photochemical stress in rats via AP-1/NF-kappaB/Caspase-1 apoptotic mechanisms.
Asunto(s)
Animales , Ratas , Alimentación Animal/análisis , Antocianinas/administración & dosificación , Antioxidantes/administración & dosificación , Western Blotting , Caspasa 1/genética , Dieta , Suplementos Dietéticos/análisis , Proteínas I-kappa B/genética , FN-kappa B/genética , Proteínas de Neoplasias/genética , Proteínas de Transporte Nucleocitoplasmático/genética , Oryza/química , Proteínas Proto-Oncogénicas c-fos/genética , Proteínas Proto-Oncogénicas c-jun/genética , Ratas Sprague-Dawley , Reacción en Cadena en Tiempo Real de la Polimerasa , Enfermedades de la Retina/etiología , Transducción de Señal/efectos de los fármacos , Factor de Transcripción AP-1/genéticaAsunto(s)
Animales , Circulación Cerebrovascular , Trastornos Cerebrovasculares/etiología , Proteínas HSP70 de Choque Térmico/genética , Humanos , Hipoxia-Isquemia Encefálica/etiología , Recién Nacido , Leucomalacia Periventricular/etiología , Isquemia Miocárdica/complicaciones , Reperfusión Miocárdica , Proteínas Proto-Oncogénicas c-fos/genética , RatasRESUMEN
Bone is a dynamic tissue that is regulated by the activity of bone-resorbing osteoclasts and bone-forming osteoblasts. Excessive osteoclast formation causes diseases such as osteoporosis and rheumatoid arthritis. Natural substances may be useful as therapeutic drugs to prevent many diseases in humans because they avoid the many side effects of treatment with chemical compounds. Here we show that tanshinone IIA isolated from Salvia miltiorrhiza Bunge inhibits the receptor activator of NF-kappaB ligand (RANKL)-mediated osteoclast differentiation of osteoclast precursors. Tanshinone IIA suppressed the expression levels of c-Fos and NFATc1 induced by RANKL. However, retrovirus-mediated overexpression of c-Fos induced the expression of NFATc1 despite the presence of tanshinone IIA and reversed the inhibitory effect of tanshinone IIA on osteoclast differentiation. Also, the introduction of osteoclast precursors with the NFATc1 retrovirus led to osteoclast differentiation in the presence of tanshinone IIA. Our results suggest that tanshinone IIA may have a role as a therapeutic drug in the treatment of bone disease such as osteoporosis.
Asunto(s)
Ratones , Masculino , Animales , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Receptor Activador del Factor Nuclear kappa-B , Ligando RANK , Proteínas Proto-Oncogénicas c-fos/genética , Fenantrenos/farmacología , Osteoclastos/citología , Factores de Transcripción NFATC/genética , Ratones Endogámicos ICR , Glicoproteínas de Membrana/genética , Factor Estimulante de Colonias de Macrófagos/farmacología , Immunoblotting , Expresión Génica/efectos de los fármacos , Regulación hacia Abajo/efectos de los fármacos , Células Cultivadas , Diferenciación Celular/efectos de los fármacos , Proteínas Portadoras/genética , Células de la Médula Ósea/citologíaRESUMEN
The heterodimeric c-Jun/c-Fos, an activator protein-1 (AP-1) has been implicated in mesoderm induction (Dong et al., 1996; Kim et al., 1998) whereas the homodimer of c-Jun was reported to be involved in neural inhibition during the early development of Xenopus embryos. During the early vertebrate development AP-1 involvement in the neural induction is still not clearly understood. We report here that AP-1 has a role in Zic3 expression, a critical proneural gene and a primary regulator of neural and neural crest development (Nakata et al., 1997; Nakata et al., 1998). AP-1 was able to induce the Zic3 gene in a dose dependent manner but other homo- or hetero-dimeric proteins, such as c-Jun/c-Jun, JunD/FosB or JunD/Fra-1 were not. The inhibition of AP-1 activity using morpholino antisenses of c-jun mRNAs blocked the Zic3 expression induced by activin. In addition, co-injection of c-jun mRNA rescued the down-regulated Zic3 expression. The promoter region of isolated Zic3 genomic DNA was found to possess several consensus-binding site of AP-1. Thus, in the functional assays, AP-1 could increase promoter activity of Zic3 gene. These findings suggest that proneural gene, Zic3 may be regulated by heterodimeric AP-1(c-Jun/c-Fos) and it may have a role in activin signaling for the regulation of neural specific gene, Zic3.
Asunto(s)
Animales , Activinas/farmacología , Secuencia de Bases , Sitios de Unión/genética , Secuencia de Consenso/genética , Dimerización , Embrión no Mamífero/metabolismo , Regulación del Desarrollo de la Expresión Génica , Proteínas de Homeodominio/genética , Datos de Secuencia Molecular , Regiones Promotoras Genéticas/genética , Proteínas Proto-Oncogénicas c-fos/genética , Proteínas Proto-Oncogénicas c-jun/genética , ARN sin Sentido/genética , Factor de Transcripción AP-1/genética , Factores de Transcripción/genética , Transcripción Genética , Regulación hacia Arriba , Proteínas de Xenopus/genética , Xenopus laevis/embriologíaRESUMEN
OBJECTIVE@#To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/R) during acute period.@*METHODS@#The model of I/R was established, and the rats was divided normal control, ischmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes.@*RESULTS@#After 10 min ischemia and 30 min reperfusion, the area of reperfusion showed a part of cardiac myocytes weak staining, and get to peak at I-60 min/R-30 min. The myocardium in normal control and ischemia groups less than 60 min showed negative staining. No changes were found in all groups by HE staining.@*CONCLUSION@#c-fos mRNA detection may become an important method for diagnosis of I/R.
Asunto(s)
Animales , Femenino , Masculino , Ratas , Medicina Legal , Hibridación in Situ , Daño por Reperfusión Miocárdica/metabolismo , Miocitos Cardíacos/metabolismo , Proteínas Proto-Oncogénicas c-fos/genética , ARN Mensajero/genética , Distribución Aleatoria , Ratas Sprague-DawleyRESUMEN
OBJECTIVE@#To study the relationship between expression of C-FOS protein and brain concussion and find a sensitive marker of diagnosis of the brain concussion.@*METHODS@#Fifty-five rats were randomly divided into brain concussion groups and control group. The expression of C-FOS protein was microscopically observed by immunohistochemical method.@*RESULTS@#There were negative expression of C-FOS protein in control group. In brain concussion group, however, positive expression of C-FOS protein in some neurons was seen at 15 min after brain concussion, and reach to the peak at 6 h after brain concussion, then decreased gradually.@*CONCLUSION@#These findings suggest that detection of C-FOS protein could be an index of diagnosis of brain concussion and a sensitive marker of timing of injury after brain concussion.
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Animales , Femenino , Masculino , Ratas , Biomarcadores , Conmoción Encefálica/metabolismo , Medicina Legal , Inmunohistoquímica , Proteínas Proto-Oncogénicas c-fos/genética , ARN Mensajero/genética , Distribución Aleatoria , Ratas Sprague-Dawley , Factores de TiempoRESUMEN
This study was performed to prove the hypothesis that oncogene expressions would have the same patterns with those of cellular growth to growth factors in FRTL-5 cells. Ribonucleic acids of FRTL-5 were extracted at 15', 30', 60' and 120' after administration of growth factors to quiescent FRTL-5, and blotted to the nitrocellulose membrane. They were hybridized with radiolabelled c-fos, c-myc and beta-actin probes. Hybridized dot blots were autoradiographed and the amount of radioactivity was measured by densitometry. Densitometric readings were used as the indices of oncogene expressions. Expressions of c-fos and c-myc were more prominent in combined administrations of TSH (10 mU/ml) and IGF-I (100 ng/ml) or IgG of Graves' disease (Graves' IgG; 1 mg/ml) and IGF-I than in combined administration of TSH and Graves' IgG. IgG of primary myxedema suppressed oncogene expressions by TSH or Graves' IgG, but not by IGF-I. From the above results, it was suggested that expressions of c-fos and c-myc to growth factors would have similar patterns with those of cell growth to growth factors in FRTL-5, and the actions of TSH and Graves' IgG would be manifested through same signal transduction system, but IGF-I would be manifested by its own.
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Ratas , Animales , División Celular/efectos de los fármacos , Línea Celular/citología , Expresión Génica/efectos de los fármacos , Enfermedad de Graves/inmunología , Sustancias de Crecimiento/genética , Inmunoglobulina G/farmacología , Factor I del Crecimiento Similar a la Insulina/farmacología , Mixedema/inmunología , Proteínas Proto-Oncogénicas c-fos/genética , Proteínas Proto-Oncogénicas c-myc/genética , ARN/análisis , Ratas Endogámicas F344 , Glándula Tiroides/citología , Tirotropina/farmacología , Factores de TiempoRESUMEN
To investigate the effects of stress on c-fos mRNA expression, rats were submitted to forced immobilization for 15, 30, 60 or 120 min before sacrifice. In situ hybridization was performed on sections containing the dorsal hippocampus with a 32P-labelled 50-base oligonucleotide probe (10(7)-10(9) cpm/micrograms) complementary to nucleotides 370-319 of rat c-fos. Forced restraint induced a time-dependent increase in c-fos mRNA expression which was most pronounced in the dentate gyrus and CA1-CA3 regions of the hippocampal formation, and which peaked after 30 min of immobilization (72.7 +/- 1.0 vs 24.1 +/- 0.8 cpm/mm2 in unrestrained animals). A positive but weaker signal was also detected in the amygdala, pyriform cortex and other parts of the cerebral cortex and habenulae. These results suggest that the hippocampal formation is activated during stress.
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Animales , Masculino , Ratas , Hipocampo , Proteínas Proto-Oncogénicas c-fos/fisiología , Estrés Fisiológico , Cerebro , Hibridación in Situ , Proteínas Proto-Oncogénicas c-fos/genética , Ratas Wistar , Restricción Física , ARN Mensajero , Estrés Fisiológico , Factores de TiempoRESUMEN
To investigate the effects of stressful stimuli on pineal gland activity, male Wistar albino rats (200-250 g, 2-4 per group) were submitted to 30 min of forced immobilization or to unilateral vibrissotomy 30 min before sacrifice. In situ hybridization was performed with a 35S-labelled 50-base oligonucleotide probe complementary to nucleotides 270-319 of rat c-fos on sections containing the pineal gland. Autoradiograms were quantified using a JAVA microdensitometer. Stressful stimuli induced a significant increase in the expression of c-fos mRNA in the pineal gland (restraint = 144.3 +/- 14.4 cpm/mm2; hemivibrissotomy = 206.7 +/- 29.5 cpm/mm2) as compared to no restraint animals (30.6 +/- 5.1 cpm/mm2), animals displaying tonic-clonic seizures after an ip (64 mg/kg) injection of pentylenetetrazole (34.0 +/- 4.7 cpm/mm2), or competition (70.6 +/- 11.4 cpm/mm2) and RNAase-treated (52.7 +/- 9.1 cpm/mm2) controls. These results raise the possibility that stressful stimuli may interfere with pineal gland function