Use of MALDI-TOF for identification and surveillance of gram-negative bacteria in captive wild psittacines / Uso do MALDI-TOF para identificação e monitoramento de bactérias Gram-negativas em psitacídeos cativos

Microbiological studies of the sanitary and health status of psittacine birds that will be reintroduced is important in evaluating whether these animals act as carriers of pathogenic agents to other animals and humans. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a faster and more accurate method to identify bacteria than conventional microbiology methods. The aim of this study was to evaluate the health status of psittacines housed in captivity, by assessment of Gram-negative bacteria from fecal microbiota through MALDI- TOF MS identification. The results indicate high frequency of Gram-negative bacteria in feces (96.5%), especially from the Enterobacteriaceae family (88.7%). The most prevalent bacteria were Escherichia coli (39.0%), Proteus vulgaris (12.2%), Klebsiella spp. (12.1%) and Raoultella ornithinolytica (8.7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. and Escherichia hermannii were isolated with lower frequency. . All these agents are potentially pathogenic for parrots and can cause systemic infections in other animals and humans. These findings reinforce that MALDI- TOF MS proved to be a rapid and accurate method of identification of the microorganism and evaluation of the health status of psittacines, providing relevant data to assist decision-making regarding the sanitary protocols in wildlife centers, and possible future reintroduction of wild birds.

Estudos microbiológicos da sanidade de psitacídeos que serão reintroduzidos são importantes para avaliar se esses animais atuam como portadores de agentes patogênicos para outros animais e humanos. A espectrometria de massa por ionização/dessorção de matriz assistida por laser/tempo de vôo (MALDI-TOF MS) é um método mais rápido e preciso para identificar bactérias na comparação com métodos convencionais de microbiologia. O objetivo deste estudo foi avaliar o estado de saúde de psitacídeos cativos, identificando bactérias Gram-negativas da microbiota fecal por MALDI -TOF MS. Os resultados indicaram alta frequência de bactérias Gram-negativas nas fezes (96,5%), principalmente da família Enterobacteriaceae (88,7%). As mais prevalentes foram Escherichia coli (39,0%), Proteus vulgaris (12,2%), Klebsiella spp. (12,1%) e Raoultella ornithinolytica (8,7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. e Escherichia hermannii foram isolados com menor frequência. Todos esses agentes são potencialmente patogênicos para os papagaios e podem causar infecções sistêmicas em outros animais e seres humanos. Esses achados reforçam que o MALDI- TOF MS é um método rápido e preciso de identificação do microrganismo e avaliação do estado de saúde dos psitacídeos, fornecendo dados relevantes para auxiliar na tomada de decisões sobre os protocolos sanitários em centros de triagem de animais selvagens e sobre a possibilidade de reintrodução futura.

First report of the aac(6)-Ib-cr gene in Providencia stuartii isolates in Brazil

Abstract INTRODUCTION: The aac(6')-Ib-cr and bla KPC genes are spreading among Enterobacteriaceae species, including Providencia stuartii, in some countries of world. METHODS: These genes were investigated in 28 P. stuartii isolates from a public hospital in Recife, Pernambuco, Brazil, by PCR and sequencing. RESULTS: The aac(6')-Ib-cr gene was detected in 16 resistant isolates, and the bla KPC gene was seen in 14. CONCLUSIONS: The presence of these genes in P. stuartii multi- and extensively drug-resistant isolates indicates that the resistance arsenal of this species is increasing, thus limiting the therapeutic options.

Isolation and identification of cholesterol-degrading intestinal bacteria by culturomics and evaluation of their functions / 生物工程学报

High cholesterol is one of the important factors inducing cardiovascular and cerebrovascular diseases. Drug therapy is the main method for reducing cholesterol, but has the disadvantages such as high cost and side effects. Studies have shown that intestinal bacteria play important roles in cholesterol metabolism. However, there are few reports on the screening and functional evaluation of cholesterol-lowering intestinal bacteria. In this study, 36 bile-tolerant bacteria were screened from healthy people stool through culturomics using bovine bile acid or artificial mixed bile acids as substrates. Taking Lactobacillus rhamnosus GG (LGG) as a positive control, three bile acid concentration groups (0 g/L, 0.3 g/L, 3 g/L) were set up to evaluate the cholesterol-lowering ability of bile-tolerant bacteria in vitro. Ten bacteria (including Proteus mirabilis, Providencia stuartii, Proteus vulgaris et al) were identified as the dominant cholesterol-lowering bacteria. Six of the above bacteria, Proteus mirabilis, Providencia stuartii, Proteus vulgaris, Proteus penneri, Wohlfahrtiimonas chitiniclastica, Providencia rettger, were evaluated for their ability to reduce triglycerides in vitro and tolerance to artificial gastric juice. Comparing with strain LGG, the six bacteria showed better triglyceride-lowering ability in vitro. With the decrease of pH value of artificial gastric juice and the increase of treatment time, the survival rate of six bacteria decreased. The above screening experiments and functional evaluation provide a basis for further development of potential cholesterol-lowering bacterial products.

Clinical significance of Providencia bacteremia or bacteriuria

No abstract available.

Clinical and microbiological features of Providencia bacteremia: experience at a tertiary care hospital

BACKGROUND/AIMS: Providencia species frequently colonize urinary catheters and cause urinary tract infections (UTIs); however, bacteremia is uncommon and not well understood. We investigated the clinical features of Providencia bacteremia and the antibiotic susceptibility of Providencia species. METHODS: We identified cases of Providencia bacteremia from May 2001 to April 2013 at a tertiary care hospital. The medical records of pertinent patients were reviewed. RESULTS: Fourteen cases of Providencia bacteremia occurred; the incidence rate was 0.41 per 10,000 admissions. The median age of the patients was 64.5 years. Eleven cases (78.6%) were nosocomial infections and nine cases (64.3%) were polymicrobial bacteremia. The most common underlying conditions were cerebrovascular/neurologic disease (n = 10) and an indwelling urinary catheter (n = 10, 71.4%). A UTI was the most common source of bacteremia (n = 5, 35.7%). The overall mortality rate was 29% (n = 4); in each case, death occurred within 4 days of the onset of bacteremia. Primary bacteremia was more fatal than other types of bacteremia (mortality rate, 75% [3/4] vs. 10% [1/10], p = 0.041). The underlying disease severity, Acute Physiologic and Chronic Health Evaluation II scores, and Pitt bacteremia scores were significantly higher in nonsurvivors (p = 0.016, p =0.004, and p = 0.002, respectively). Susceptibility to cefepime, imipenem, and piperacillin/tazobactam was noted in 100%, 86%, and 86% of the isolates, respectively. CONCLUSIONS: Providencia bacteremia occurred frequently in elderly patients with cerebrovascular or neurologic disease. Although Providencia bacteremia is uncommon, it can be rapidly fatal and polymicrobial. These characteristics suggest that the selection of appropriate antibiotic therapy could be complicated in Providencia bacteremia.

Spontaneous Bacterial Peritonitis with Septicemia with Providencia rettgeri and Clostridium perfringens / 대한임상미생물학회지

We report a suspicious case of spontaneous bacterial peritonitis (SBP) caused by Providencia rettgeri and Clostridium perfringens in a patient with alcoholic cirrhosis. The patient presented with altered mentality and was taken to the emergency room. He was diagnosed with SBP after abdominal paracentesis and computed tomography and was treated with ceftriaxone and metronidazole. The pathogens were identified under suspicion of polymicrobial infection because of Gram-staining discrepancies between broth from blood culture bottles and colonies on solid media. He died of septic shock despite transfer to the intensive care unit. Although we could not conclude which organism had the leading role in this case of SBP and septicemia, we did verify the importance of Gram staining in a microbiology laboratory in terms of quality assurance.

Providencia rettgeri Prostatic Abscess Cured by Medical Therapy

A prostatic abscess is a rare, but potentially serious disease. The mainstay of treatment for the prostatic abscess is antibiotic administration and drainage. Here, we experienced a 66-year-old man with a prostatic abscess caused by Providencia rettgeri, which has not been reported as a pathogenic agent of a prostatic abscess. He was cured using antibiotics, without surgical drainage. This case suggests that the appropriate selection of patients for antibiotic therapy may provide an excellent prognosis.

A Case of Providencia rettgeri Sepsis in a Patient with Cervical Cord Injury

Providencia rettgeri is a member of Enterobacteriacea that is known to cause urinary tract infection (UTI), septicemia, and wound infections, especially in immunocompromised patients and in those with indwelling urinary catheters. We experienced a case of UTI sepsis by Providencia rettgeri in a patient with spinal cord injury. The patient had only high fever without urinary symptoms or signs after high dose intravenous methylprednisolone. The laboratory results showed leukocytosis (21,900/microL, segmented neutrophils 91.1%) and pyuria. Cefepime was given empirically and it was switched to oral trimethoprim-sulfamethoxazole because P. rettgeri was identified from blood and urine culture which was susceptible to TMP-SMX. The patient was improved clinically but P. rettgeri was not eradicated microbiologically. To the best of our knowledge, this is the first case report on sepsis caused by Providencia rettgeri in Korea.

Isolation, diagnosis and antibiotics susceptibility of the most prevalent enterobacteriaceae genera from aleppo environment

Enterobacteriaceae is a group of gram-negative bacteria that can cause infections of the digestive tract or other organs of the body. The group Enterobacteriaceae includes the bacteria Enterobacter, Escherichia, Klebsiella, Proteus, Providencia, Salmonella, Serratia, Shigella, and Yersinia. Although most of these bacteria normally inhabit the intestines, nevertheless it is the one of most important pathogenic which cause dangerous diseases such as: meningitis, infections of the urinary and respiratory tract, septicemia, and endocarditic. 10 bacterial genera have been isolated from different environmental aereas [water, soil, plants] in south of Syria. We used many culture media [general, differential, selective media] to isolate pure bacterial colony, then identify them by using microtetration plates [Micronaut-E] of Micronaut-Mltiscan System /Type: 355, Serial: 355052445/ [Merlin-Diagnostika, Bornheim, Germany]. We have studied the antimicrobial susceptibility for 420 bacterial isolation [related to 10 speacies] against 19 antibiotics, by using SB-plate of automated broth microtiter system [MERLIN Company]. Results indcate that there are great changes of antimicrobial resistance against many antibiotics, often increase and sometimes decrease in susceptibility

Production of VIM-2 Type Metallo-beta-Lactamase in Urinary Isolates of Providencia rettgeri / 대한진단검사의학회지

BACKGROUND: VIM-2 type metallo-beta-lactamase (MBL) producing strains are presently spreading to Pseudomonas spp., Acinetobacter spp. and even to Enterobacteriaceae such as Serratia marcescens, Enterobacter cloacae and Klebsiella pneumoniae in Korea. Recently we determined the phenotype and the genotype of three MBL-producing Providencia rettgeri isolated from urinary specimen of three patients with neurosurgical ward, and analyzed the blaVIM-2 containing integron of a P. rettgeri CBU852. METHODS: EDTA-disk synergy test was used for the screening of MBL, and the PCR for blaIMP-1, blaVIM-1 and blaVIM-2 was performed. The minimal inhibitory concentration of those isolates was determined by broth microdilution method, and the genomic DNA fingerprinting analysis was performed by random amplified polymorphic DNA (RAPD). The sequence of the blaVIM-2 containing integron was determined. RESULTS: Three P. rettgeri with reduced imipenem susceptibility showed the positive EDTA-disk synergy test and blaVIM-2 was detected by PCR. Antimicrobial susceptibility test showed the resistance to all beta-lactams tested, ciprofloxacin and aminoglycoside such as gentamicin, tobramycin and amikacin, indicating multidrug resistance of those isolates. RAPD analysis showed the identical DNA fingerprint of those three isolates. The novel class 1 integron, including aacA4, blaVIM-2, orf "ii" and orf "iii", was detected in a P. rettgeri CBU852. CONCLUSIONS: In this study, the multidrug resistant P. rettgeri CBU852 had blaVIM-2 containing novel class 1 integron. The emergence of blaVIM-2 producing P. rettgeri could compromise the use of carbapenem in treatment of infections caused by MBL producing bacteria. To our knowledge, this is the first report that VIM-2 MBL gene has been detected in P. rettgeri.

Interpretation of Antimicrobial Susceptibility Test of Enterobacteriaceae to beta-lactams with Expert System / 대한진단검사의학회지

BACKGROUND: At antimicrobial susceptibility testing (AST), clinical isolates may appear susceptible sometimes to the antibiotics that are clinically ineffective or due to technical errors in the testing. So an interpretive reading of AST should be done, but most hospitals do not perform it routinely. Here, we developed and evaluated a computerized expert system to interpret AST of Enterobacteriaceae to beta-lactam antibiotics. METHODS: We made a rule-based expert system according to the natural resistance of the members of Enterobacteriaceae and the common phenotypes of resistance mechanisms for Enterobacteriacae. Antimicrobial suceptibility testings were performed using the disk diffusion method with 12 beta-lactam antibiotics for a total of 1, 016 clinical isolates. Then we compared the raw and expert results of AST. RESULTS: An overall discrepancy rate due to natural resistance was 5.9%; 10.4% for Klebsiella spp. and Citrobacter diversus, 15.0% for Enterobacter spp., 2.6% for Serratia marcescens, 31.6% for Morganella morganii and Providencia stuartti. Accoriding to acquired antimicrobial resistant mechanisms, overall resistant discrepancy was 21.8%; 18.8% for Escherichia coli, Proteus mirabilis, Salmonella and Shigella spp., 25.9% for Citrobacter diversus and Klebsiella spp., 21.6% for Citrobacter freundii and Enterobacter spp., 45.6% for Morganella morganii, 10.0% for Proteus vulgaris, 12.2% for Serratia spp.. CONCLUSIONS: These results indicate that the application of the expert system for interpretation of antimicrobial susceptibility test may provide more reliable data for the treating physician. Additional information should be applied on the software for new resistant mechanisms or some misinterpretive readings.

Current Bacteriology of Chronic Suppurative Otitis Media / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: It is important to know the current trend of resistance rate and species of pathogens in order to select appropriate antibiotics in the management of chronic suppurative otitis media. The purpose of this study is to investigate the current bacteriology of chronic supprative otitis media. SUBJECTS AND METHOD: A retrospective study was performed on the bacteriologic results of 169 out-patients of chronic otitis media with otorrhea who visited the department of otolaryngology at the Kyung Hee Medical Center from Jan 2000 to Dec 2002. RESULTS: The most common pathogenic organisms were Staphylococcus, Pseudomonas, Proteus and Providencia. The prevalence of MRSA was found to be 61.0 per cent of isolated Staphylococcus aureus. CONCLUSION: We investigated the current bacteriology of chronic suppurative otitis media and could not find remarkable changes in comparison with the results reported recently. Continuous and periodic studies on bacteriology and sensitivity tests should be performed for effective treatment of chronic suppurative otitis media.

Análise dos fatores de virulência e do potencial patogênico em amostras de providencia alcalifaciens com capacidade de invadir ou näo células cultivadas in vitro / Analysis of the virulence factors and the pathogenic potential of Providencia alcalifaciens strains presenting or not invasive ability to in vitro cultured cells

Providencia alcaffaciens é um enteropatógeno invasor e tem sido isolado) de casos de diarréia em crianças e adultos] em países erre desenvolvimento. Estudos anteriores realizados em nosso laboratório demonstraram que as amostras invasoras eram capazes de aderir a células HeLa. A análise desta interação à microscopia eletrônica de varredura revelou uma discreta projeção de membrana e formação de pedestal nos sítios de aderência bacteriana Entretanto não existem ainda relatos sobre os componentes bacterianos envolvidos na expressão, do fenótipo de aderência) e invasão de P. alcalifacíens Portantoa os objetivos deste estudo foram pesquisar os fatores de virulência relacionados ao fenótipo de aderência e invasão em amostras de P. alcalifacíens, bem como avaliar a presença de outros mecanismos de patogenicidade em amostras invasoras (INV+) e não invasoras (INV; isoladas de casos de diarréia, em São Paulo. Neste trabalho foram analisadas 30 amostras sendo que em I 5 amostras foi confirmada a capacidade de aderir e invadir células HeLa previamente identificada, Todas estas 15 amostras foram também capazes de aderir e invadir células Caco-2 apresentando um padrão de aderência semelhante ao tipo localizada (AL de Escherichia coli enteropatogênica (EPEC', Entretanto ai interação as células Caco-2I ocorreu erre menor freqüência do que a observada em células HeLa. Nas demais amostras, não...(au)

Comparação das atividades antimicrobianas de meropenem e imipenem/cilastatina: o laboratório necessita testar rotineiramente os dois antimicrobianos? / Comparative antimicrobial activity between meropenem and imipenem/cilastatina: does the clinical laboratory need to test both imipenem and meropenem routinely?

O meropenem e o imipenem representam os ßðlactâmicos com maior espectro e potência antimicrobiana, e são os únicos carbapenêmicos disponíveis para uso clínico no Brasil, nos Estados Unidos e na Europa. O meropenem apresenta atividade in vitro superior contra gramðnegativos, enquanto que o imipenem é discretamente mais ativo contra gramðpositivos. Os objetivos deste estudo são comparar as atividade in vitro destes dois carbapenêmicos e avaliar a necessidade de o laboratório clínico testáðlos em sua rotina. Os resultados da avaliação dos padrões de sensibilidade de 2.144 bacilos gramðnegativos pela técnica de microdiluição em caldo foram analisados. Contra enterobactérias, o meropenem apresentou atividade pelo menos oito vezes maior que o imipenem. Contra Pseudomonas aeruginosa, o meropenem (CIM50 de 1 µg/ml) também apresentou atividade superior à do imipenem (CIM50 de 1 µg/ml para ambos). Somente 2,7 por cento das amostras avaliadas apresentaram resultados discordantes entre os dois carbapenêmicos em termos de categoria de sensibilidade ð isto é, foram sensíveis a um e resistentes ao outro. Quarenta e seis amostras (2,14 por cento) foram sensíveis ao meropenem e resistentes ao imipenem, enquanto que somente 12 amostras (0,55 por centro) apresentaram sensibilidade ao imipenem e resistência ao meropenem. A grande maioria dos resultados discordantes (91,4 por centro) ocorreu entre as amostras de bacilos gramðnegativos nãoðfermentadores da glicose (BGNðNF). Entre as 1.350 enterobactérias testadas houve apenas cinco resultados discordantes (0,37 por cento), enquanto que entre os BGNðNF ocorreram 53 (6,67 por cento). Além disso, em cerca de 80 por cento, as amostras foram sensíveis ao meropenem e resistentes ao imipenem. Os resultados deste estudo indicam que o laboratório de microbiologia pode testar apenas um dos carbapenêmicos contra enterobactérias, considerando para o outro o mesmo resultado. É importante que os resultados dos dois carbapenêmicos sejam colocados no relatório, para que o médico possa escolher aquele que achar mais adequado. Por outro lado, para os BGNðNF, o laboratório deve realizar o teste de sensibilidade com os dois carbapenêmicos separadamente

A Case of Acute Otitis Media Caused by Providencia stuartii / 대한임상병리학회지

Providencia stuartii is occasionally isolated from patients with chronic otitis media, however it has not been reported as a cause of acute otitis media so far. We recently isolated P. stuartii from the pus specimen of right middle ear of a 2-year-old patient with acute otitis media. The patient was admitted because of right-sided otorrhea for 2 weeks. The symptom was not relieved by first-line empirical antibiotics (amoxicillin-clavulanic acid and netilmicin), but it was subsided dramatically with the changed antibiotics (ceftriaxone and amikacin); the isolate was susceptible to these two antibiotics in in vitro susceptibility testing. To our knowledge, this is the first case report of acute otitis media caused by P. stuartii.

In Vitro Activities of Cefpirome Against the Bacteria Isolated from the Patients in ICU, Oncology and Hematology Units / 감염

BACKGROUND: Infections due to antimicrobial resistant bacteria pose serious problem in the care of the patients in intensive care units, oncology and hematology. It was to determine the prevalent species and cefpirome susceptibilities of the current isolates from these patients. METHODS: Bacteria isolated from patients in the intensive care units, oncology and hematology in a fertiary care university hospital in 1997 were analyzed for the prevalent species. Antimicrobial susceptibility of the strains, most of which were isolated in 1997, was tested by the NCCLS agar dilution method. RESULTS: The proportion of potential pathogens isolated were: Staphylococcus aureus 16.1%, Acinetobacter baumannii 13.6%, Enterobacter-Serratia-Citrobacter group 12.1%, Enterococcus spp. 11.0% and Pseudomonas aeruginosa 10.4%. The lowest resistance rates were : A.baumannii to ampicillin/sulbactam (38%), P. aeruginosa to ceftazidime (37%), Providencia spp. to ceftriaxone (7%), S. marcescens to ceftazidime and cefpirome (10%), and E. coli (3%), K. pneumoniae (10%), E. cloacae (17%) and C. freundii (7%) to cefpirome. All isolates of penicillin-resistant Streptococcus pneumoniae were susceptible to cefpirome. CONCLUSION: The in vitro test suggests that cefpirome should be more useful than the other generations of cephalosporins for the treatment of various nosocomial infections including those due to the 3rd generation cephalosporin-resistant E. cloacae, S. marcescens, C. freundii and penicillin-resistant S. pneumoniae.

In Vitro Activities of Cefminox against Enterobacteriaceae Species, Haemophilus influenzae, and Methicillin-Susceptible Staphylococcus aureus / 감염

BACKGROUND: Recent clinical isolates of bacteria often produce various beta-lactamases, for example, extended spectrum beta-lactamase (ESBL) by some species of Enterobacteriaceae, TEM beta-lactamase by Haemophilus influenzae, and penicillinase by methicillin-susceptible Staphylococcus aureus (MSSA). Cefminox, a commonly used cephamycin, is stable to various beta-lactamases, but its activity against recent clinical isolates has not been evaluated. The aim of this study was to determine the activities of cefminox against recent clinical isolates of Enterobacteriaceae, H. influenzae, and MSSA. METHODS: The organisms, isolated from Severance Hospital patients during 1997 to 1998, were kept frozen until the test. Antimicrobial susceptibility was determined by the NCCLS agar dilution method. RESULTS: All 30 isolates of Escherichia coli and 90% of 30 Klebsiella pneumoniae isolates were susceptible to cefminox, cefotetan and amikacin. All of the Enterobacter cloacae and Serratia marcescens, and 86% of Citrobacter freundii isolates were susceptible to amikacin. All of the 15 isolates each of Proteus mirabilis, P. vulgaris and Morganella morganii were susceptible to cefminox, cefotetan, cefotaxime, ceftazidime, and aztreonam, while all of the Providencia spp. were susceptible to ceftazidime and aztreonam. All of the 29 H. influenzae isolates were susceptible to cefminox, cefotaxime, and levofloxacin, while all MSSA isolates were susceptible to cefoxitin and cotrimoxazole. CONCLUSION: Cefminox is more active than the other beta-lactams against Enterobacteriaceae including, ESBL- producing E. coli, and K. pneumoniae.

Isolation Rate and Biochemical Reaction of Enterobacteriaceae / 대한임상미생물학회지

BACKGROUND: In clinical microbiology the accurate and rapid identification of members of the family Enterobacteriaceae is essential for diagnostic and therapeutic purposes and for epidemiologic studies. Accuracy of identification system depends mainly on data base such as positive rate of biochemical reactions, relative frequency of occurrence of biotype, and isolation frequency of microorganisms. The purpose of this study was to analyze the isolation rate and biotype frequency of the family Enterobacteriaceae isolated from tertiary care hospital in Korea. METHODS: Isolation frequency of the family Enterobacteriaceae isolated from clinical specimens during the period of January 1998 to June 1998 were analyzed. And biochemical phenotypes of 2,022 isolates tested by 10 tube system consisting of 14 conventional biochemical tests were also analyzed. RESULTS: Isolation rate of the family Enterobacteriaceae to the genus level in order of decreasing frequency were Escherichia (37.0%), Serratia (15.9%), Klebsiella (14.9%), Enterobacter (11.1%), Providencia (8.1%), Citrobacter (2.8%), Proteus (2.5%), Morganella (2.4%), Salmonella (2.4%), and Cedecea (0.7%). Among the genus of the family Enterobacteriaceae, Budvicia, Edwardsiella, Ewingella, Hafnia, Kluyvera, Leminorella, Moellerella, Shigella, Tatumella, Xenorhabdus, Yersinia, and Yokenella were not isolated. The number of species and genus of the family Enterobacteriaceae by this study were 48 and 12, respectively. Over 95% of all clinical isolates belonged to only 25 species. CONCLUSIONS: Although these data about frequency of relative isolation rate and biotype patterns of the family Enterobacteriaceae is inadequate according to species and genus, yet these data will be utilized for the application and development of identification method of the family Enterobacteriaceae.

Bacteriologic Study of Chronic Suppurative Otitis Media / 대한이비인후과학회지

BACKGROUND: Chronic suppurative otitis media is one of the most common disease in the otolaryngologic field. It is important to choose of antibiotics in the management of chronic suppurative otitis media. OBJECTIVES: Bacteriologic studies can make it possible to use appropriate antibiotics. MATERIALS AND METHODS: The bacteriologic study was made on 98 cases of chronic suppurative otitis media who visited the department of otolaryngology, Dae Dong Hospital in Pusan from Nov. 1989 to Dec. 1995 and the following results were obtained. RESULTS: 1) In 90 cases in which pathogenic organism was isolated, single infection was 81 cases(90%) and mixed infection was 9 cases(10%). 2) The most frequent pathogenic organism was Staphylococcus(46.5%) and Proteus(16.1%), Pseudomonas(14.1%), Providencia(10.1%) were the next. 3) In the aspect of the sensitivity to antibiotics, Ciprofloxacin, Ceftriaxon and Amikacin were sensitive drug generally. 4) Staphylococcus was sensitive to Vancomycin and Ciprofloxacin. 5) Proteus was sensitive to Amikacin, Ciprofloxacin, and Ceftriaxon. 6) Pseudomonas was sensitive to Ciprofloxacin, Amikacin and Piperacin. 7) Providencia was sensitive to Ciprofloxacin, Ceftriaxon, Amikacin and Piperacin. 8) Methicillin Resistant Staphylococcus Aureus(MRSA) was 19 Strains(45.5%). CONCLUSION: Recently, pathologic strain and it's sensitivity to antibiotics has changed. So, we recommand that periodic bacteriologic study and sensitivity test should be done for effective management of chronic otitis media.