RESUMEN
Objective@#This study aimed to investigate the effects of @*Methods@#In this study, 0.1% DMG was supplemented in 20% casein diets that were either folate-sufficient (20C) or folate-deficient (20CFD). Blood and liver of rats were subjected to assays of Hcy and its metabolites. Hcy and its related metabolite concentrations were determined using a liquid chromatographic system.@*Results@#Folate deprivation significantly increased pHcy concentration in rats fed 20C diet (from 14.19 ± 0.39 μmol/L to 28.49 ± 0.50 μmol/L; @*Conclusion@#DMG supplementation exhibited hypohomocysteinemic effects under folate-sufficient conditions. By contrast, the combination of folate deficiency and DMG supplementation has deleterious effect on pHcy concentration.
Asunto(s)
Animales , Masculino , Ratas , Biomarcadores/metabolismo , Cromatografía Liquida , Dieta , Suplementos Dietéticos , Deficiencia de Ácido Fólico/metabolismo , Homocisteína/metabolismo , Hígado/metabolismo , Distribución Aleatoria , Ratas Wistar , Sarcosina/metabolismoRESUMEN
Discutem-se aqui as formas de encaminhamento de pacientes ao Hospital Adauto Botelho, localizado em Cariacica, Espírito Santo. A pesquisa se deu por meio de prontuários médicos datados desde a inauguração em 1954 e de depoimentos de pessoas que trabalharam lá durante a segunda metade do século XX. Foram analisados 102 prontuários e entrevistadas quatro pessoas. A pesquisa dos prontuários mostra forte inserção da Chefatura de Polícia no processo de internação. As falas dos entrevistados reiteram esse ponto, mostrando também a longa duração das internações. São as histórias de vida dos internos que dão o tom deste trabalho. Conclui-se, a partir delas, que o Hospital Adauto Botelho, mais que uma instituição de tratamento, era um espaço de confinamento.
This paper discusses the procedures for referring patients to Adauto Botelho Hospital, in Cariacica, Espírito Santo state, Brazil. The research is based on the medical records since its inauguration in 1954 and statements by people who worked there in the second half of the twentieth century. One hundred and two records were analyzed and four people were interviewed. The records revealed the active involvement of the Chief of Police in hospitalizations. The interviews corroborate this, while also showing the long duration of the hospitalizations. The tone of the paper is set by the life stories of the people hospitalized there. The conclusion is that this hospital served not so much for treatment as for confinement.
Asunto(s)
Animales , Proteínas de Neoplasias/metabolismo , Extensión de la Cadena Peptídica de Translación , ARN Polimerasa I/metabolismo , Factores Generales de Transcripción , Transcripción Genética , Factores de Elongación Transcripcional , Factores de Transcripción/metabolismo , Secuencia de Aminoácidos , ADN Polimerasa II/metabolismo , Detergentes/metabolismo , Electroforesis en Gel de Poliacrilamida , Datos de Secuencia Molecular , Sarcosina/análogos & derivados , Sarcosina/metabolismo , XenopusRESUMEN
Glyphosate application resulted in a decline in soil pH with consequent increase in soil mycoflora suggesting an indirect relationship. Though the composition of mycoflora unchanged, species of aspergilli, fusaria, penicillia and Trichoderma were predominant. HPLC, IR analysis revealed the presence of sarcosine derivative as an intermediary of glyphosate degradation in soil.