Maturation and development of fetal pig intestinal tissue in immunodeficient mice

Purpose: This study aimed to compare the degree of maturation and development of fetal pig segmental intestinal tissue with that of spheroids created by in-vitro reaggregation of dissociated fetal intestinal cells after transplantation into immunodeficient mice. Methods: Fetal pig small intestines were transplanted as segmental grafts into the omentum and subrenal capsules of immunodeficient mice or enzymatically treated to generate single cells. Spheroids made by in-vitro reaggregation of these cells were transplanted into the subrenal capsules of immunodeficient mice. The segmental grafts and spheroids were harvested four and eight weeks after transplantation, and the structural maturity and in-vivo development of these specimens were histologically evaluated. Results: The spheroids were engrafted and supplied blood vessels from the host mice, but an intestinal layered structure was not clearly observed, and there was almost no change in size. On the other hand, the segmental grafts formed deep crypts in the mucus membrane, the inner circular layer, and outer longitudinal muscles. The crypts of the transplanted grafts harvested at eight weeks were much deeper, and the smooth muscle layer and the enteric nervous system were more mature than those of grafts harvested at the fourth week, although the intestinal peristaltic wave was not observed. Conclusions: Spheroids created from fetal small intestinal cells could not form layered structures or mature sufficiently. Conversely, segmental tissues structurally matured and developed after in-vivo transplantation and are therefore potential grafts for transplantation.

Techniques of fragile renal organoids transplantation in mice

Purpose: This study aimed to develop a microsurgical technique to transplant extremely fragile renal organoids in vivo, created by in-vitro reaggregation of metanephros from fetal mice. These organoids in reaggregation and development were examined histologically after transplantation under the renal capsule. Methods: Initially, metanephros from fetal mice were enzymatically treated to form single cells, and spheroids were generated in vitro. Under a microscope, the renal capsule was detached to avoid bleeding, and the outer cylinder of the indwelling needle was inserted to detach the renal parenchyma from the renal capsule using water pressure. The reaggregated spheroid was aspirated from the culture plate using a syringe with an indwelling needle outer cylinder and carefully extruded under the capsule. Pathological analysis was performed to evaluate changes in reaggregated spheroids over time and the effects of co-culture of spinal cord and subcapsular implantation on maturation. Results: In vitro, the formation of luminal structures became clearer on day 5. These fragile organoids were successfully implanted without tissue crapes under the renal capsule and formed glomerular. The effect of spinal cord co-transplant was not obvious histrionically. Conclusions: A simple and easy method to transplant fragile spheroids and renal under the renal capsule without damage was developed.

Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice

ABSTRACT PURPOSE: To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model. METHODS: Two groups were constituted: The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups: Tx, IPC-R, Fk, IPC-Fk, in both strains. Intestinal grafts were stained with hematoxylin and eosin and immunohistochemically. RESULTS: The graft development evaluation in ISO group showed that IPC-R reduced the development compared with ISO-Tx (5.2±0.4 vs 9.0±0.8) and IPC-R-Fk increased the graft development compared with IPC-R (11.2±0.7 and 10.2±0.8). In ALO group, IPC-Fk increased the development compared with ALO-Tx and ALO with IPC-R (6.0±0.8, 9.0±1.2, 0.0±0.0, 0.5±0.3). The PCNA expression was increased in ISO group treated with Fk and IPC-R compared to other groups (12.2±0.8 vs Tx: 8.8±0.9, IPC-R: 8.0±0.4 and Fk: 9.0±0.6). The graft rejection was lower in groups treated with IPC-R (-18%), Fk (-68%) or both (-61%) compared with ALO-Tx. CONCLUSION: Remote ischemic preconditioning showed benefic effect even associate with Tacrolimus on the development and acute rejection of the fetal small bowel graft in the Isogenic and Allogenic transplants.

Estudo estrutural do pênis de fetos humanos normais e anencéfalos no segundo trimestre de gestação / Structural study of the penis of normal and anencephalic human fetuses in the second trimester of pregnancy

A anecencefalia é o Defeito do Tubo Neural (DTN) mais severo em fetos humanos. Há uma demanda crescente para reposição tissular em doenças crônicas e cirurgias reconstrutoras. Tecidos fetais têm sido utilizados como substitutos para órgãos sólidos. Comparar a estrutura e morfologia do corpo cavernoso e corpo esponjoso de pênis de fetos humanos anencéfalos e de controle a fim de propor um novo modelo para estudos biológicos e transplantes teciduais. Foram estudados 11 pênis de fetos de controle de 14 a 23 Semanas Pós Concepção (SPC), e cinco pênis de fetos anencéfalos de 18 a 22 SPC. Os órgãos foram removidos e processados pelas técnicas histo e imunohistoquímicas rotineiras. A análise do tecido conjuntivo, células musculares lisas e fibras elásticas foram realizadas em lâminas dos espécimes. Os dados foram expressos em Densidade de àrea (Da) utilizando-se um software de processamento digital. As médias foram comparadas utilizando-se o Teste - T não pareado e quando aplicável, a regressão linear simples foi utilizada. Foi considerada significância estatística se p<0,05. O septo intercavernoso encontrava-se presente em todas as amostras. Não foram observadas diferenças da Da do tecido colágeno e musculatura lisa dos pênis de fetos anencéfalos quando comparados aos normais. A regressão linear simples sugere que durante o desenvolvimento humano há um aumen2to gradual do tecido colágeno (R2=0,45) e uma diminuição da musculatura lisa (R =0,62) no corpo cavernoso de ambos os grupos. A elastina encontrava-se presente apenas em fetos a partir da 20ª SPC. Não houve diferença na estrutura da genitália entre fetos normais e enencéfalos. Apresença da elastina em fetos a partir da 20ª SPC é um dado objetivo da manutenção da capacidade de ereção nestes grupos. A histo e imunohistoquímica sugerem que o desenvolvimento do pênis destes fetos encontra-se inalterado. Futuros estudos deverão ser realizados com o objetivo de avaliar fetos anencéfalos como um potencial ...

Anencephaly is the most severe neural tube defect (NTD) in human fetuses. There is an increasing need for tissue replacement in chronic diseases and reconstructive surgeries. Fetal tissues have been used as a substitute for native organs. Compare the structure and morphology of the corpora cavernosa and spongiosum of penises from anencephalic and normal human fetuses to propose a new model for biological studies and tissue transplantation. We studied 11 penises from normal human fetuses, aged 14 to 23 weeks post-conception (WPC), and 5 penises from anencephalic fetuses, aged 18 to 22 WPC. The organs were removed and processed by routine histological and immunolabeling techniques. Analysis of connective tissue (Cot), smooth muscle (SMC) and elastic fibers (EF) were performed in sections. Data were expressed as area density (Ad) using digital processing and software. Means were statistically compared using the unpaired t test and linear regression was performed. Statistical significance was considered if p < 0.05. The Intracavernosal septum was present in all samples. We did not observe differences in the Ad of Cot and SMC in the penises of anencephalic fetuses when compared to normal ones. The simple linear regression suggested that during human development there is a gradual increase in Cot (R2= +0.45) and a decrease of SMC (R2=- 0.62) in the corpora cavernosa in both groups studied. Elastin was observed only in fetuses from 20th WPC. There was no difference in the structure of the corpora cavernosa and corpus spongiosum of anencephalic fetuses compared to normal ones. Elastin was documented from 20th WPC, which suggests the maintenance of erectile function. Histochemistry and immunolabeling suggested that penile shaft development is maintained and unaltered in anencephalic fetuses. Further studies should be performed to analyze anencephalic fetuses as a potential tissue donating group and a model for biological studies

The effect of danshen on the angiogenesis of the frozen-thawed human fetal ovarian tissue after transplantation / 中国应用生理学杂志

<p><b>AIM</b>(1) To investigate the mRNA expression of the key angiogenic growth factors in the grafts after transplantation. (2) To investigate the potential impact of danshen (Chinese traditional medicine) administration on grafts angiogenesis.</p><p><b>METHODS</b>The frozen-thawed ovarian tissue from aborted fetus were xenografted into the renal capsule of the nude mice, recovered 48 h, 7 d and 28 d after respectively. Either danshen or saline (as the control) was administered after transplantation.</p><p><b>RESULTS</b>The mRNA levels of VEGF showed a temporary raise in 48 h after transplantation, then decreased in one week, and no significant difference was fund between the control group and danshen group. Ang-2 was increased in 48 h after transplantation, when Danshen group was significantly higher than the control group (P < 0.05). The microvessel density significantly increased in all the tissues after transplantation. The control group peaked on day 7 after transplantation, while danshen group peaked in 48 h and kept correspondingly steady after that.</p><p><b>CONCLUSION</b>Early angiogenesis began within 48 h after transplantation of the thawed human fetal ovarian tissue, and its microvessel density peaked within the first week after transplantation. Our results also suggested that the use of danshen injection in conjunction with transplantation could facilitate revascularization of the grafts.</p>

Research progression of tissue transplantation and functional reconstruction of spinal cord / 中国骨伤

Functional reconstruction of injured spinal cord depends on its structure restoration,tissue transplantation is the most important strategy in medicine field at present. The tissue applied for transplantation including peripheral nerves, embryonic spinal cord, cellular transplantation and gene organization. However, the results exist dissension. The report overviews the status quo of tissue transplantation, intended to strengthen the recognition of treatment of spinal cord injury.

Regeneration of kidney tissue using in vitro cultured fetal kidney cells

Transplanting fetal kidney cells (FKCs) can regenerate kidney. This requires in vitro expansion in cell number to acquire enough cells for transplantation. However, FKCs may change their cellular characteristics during expansion and, thus, may not regenerate kidney tissue upon transplantation. We investigated how cell culture period affects cellular characteristics and in vivo regenerative potential of FKCs. As the passage number increased, cell growth rate and colony forming ability decreased while senescence and apoptosis increased. To examine in vivo regenerative potential, FKCs cultured through different numbers of passages were implanted into the parenchyma of kidneys of immunodeficient mice using fibrin gel for 4 wk. Histological analyses showed passage-dependent kidney tissue regeneration, and the regeneration was better when cells from lower number of passages were implanted. This result shows that in vitro culture of FKCs significantly affects the cell characteristics and in vivo tissue regenerative potential.

Study of xenotransplantation of fetal pig skin precursor tissue / 中华烧伤杂志

<p><b>OBJECTIVE</b>To select the optimal pregnancy time window of embryonic pig skin precursor tissue for xenotransplantation and study its ability in wound repair.</p><p><b>METHODS</b>Skin precursor tissues were obtained from pig fetus of fetal age of 35, 42, 56, 70 days, and were minced into microskin and transplanted to dorsal wounds of BALB/c nude mice, then they were covered with residual skin after plastic surgery of patients or adult pig skin (white). The characteristics of growth and development were observed after transplantation. Pathological examination was performed on 6 and 12 post operation weeks respectively to observe the tissue structure and tumorigenicity.</p><p><b>RESULTS</b>Skin precursor tissues from fetal pig survived and developed after transplantation, and the microskin fused. New tissue area from skin precursor tissues with fetal age of 42 days was (47 +/- 6) mm2, which was higher than that of 35 days (18 +/- 8 mm2), 56 days (31 +/- 12 mm2), 70 days (20 +/- 8 mm2, P < 0.05). The skin precursor developed into "intact skin" with hair, sebaceous glands and sweat glands, and melanocytes were also detected in epidermis. The newly-grown skin tissue included epidermal and dermal layer, and obvious dermal papillae. Teratoma was not found after transplantation in skin precursor tissue with fetal age of 56, 70 days.</p><p><b>CONCLUSION</b>Fetal pig skin precursor tissue with fetal age of 56 days can be used to repair wound as xenotransplantation.</p>

Comparative study of embryo donation from viewpoint Of medical ethics and medical law

The knowledge of people about new methods of Infertilitys treatment increase by birth of first child born from invitro fertilization method in 1978. Use of these methods brings hope for many couples that think they never can have a baby. Some of these methods are egg donation, sperm donation and embryo donation that have been introduced as "surrogate pregnancy" and one of the best method of Infertility s treatment. "principles of surrogate pregnancy" and its stipulation is mentioned in relevant statutes and executive regulations, but the important issues are the certainty of healthiness of donor and donee and conformity of outwards, bloody, social, cultural and religious features and patterns. First thing in this matter is awareness of couples about the condition of the gamete and Embryo donation so that the possible risks, percentage of success, relevant costs, subsequent legal and jurisprudential Issues, secrecy of the donor and donee's identity and healthiness of gamete and embryo can be evaluated. These new methods along with the therapeutic aspects have complex ethical and legal aspects that need to be gazed and regarded in the respect of religious, social and cultural conditions of each country

How to improve the survival of the fetal ventral mesencephalic cell transplanted in Parkinson's disease? / 神经科学通报·英文版

It has been extensively confirmed that fetal ventral mesencephalic cell (VMC) transplantation can ameliorate the symptoms of Parkinson's disease (PD). But there are still several problems to be resolved before the extensive clinical application of this technology. The major limitations are the poor survival of grafted dopamine (DA) neurons and restricted dopaminergic reinnervation of host striatum. Some attempts have been made to solve these problems including use of some trophic factor and co-transplantation with neural/paraneural origins. The purpose of this review is to overview advances of the means improving the survival of grafts and their current limitations.

Trasplante de células progenitoras hematopoyéticas en pediatría: Una alternativa de tratamiento en inmunodeficiencias primarias / Hematopoietic stem cell transplant in combined immunodeficiency syndromes of childhood: An optimal treatment for primary immunodeficiency

The congenital immunodeficiency disorders in which the defect has been clearly traced to the stem cell can be cured with allogeneic stem-cell transplantation (SCT) from an unaffected donor. Widespread application of this treatment modality has been tempered by the fact that risk-benefit considerations do not always favor a procedure that carries a significant risk for morbidity and mortality. Some malignant disorders of childhood eventually have to be treated by an autologous or allogeneic SCT, however nonmalignant disorders can also be treated with this approach. This article reviews the current status of SCT for nonmalignant inherited immunodeficiency disorders.

Tradicionalmente el trasplante de células progenituras hematopoyéticas (TCPH) se ha utilizado en pacientes pediátricos para el tratamiento de padecimientos malignos. Sin embargo, también existen indicaciones y experiencia para padecimientos benignos dentro de los cuales se encuentran los síndromes de inmunodeficiencia combinada primaria. Estos síndromes de la infancia constituyen una serie de padecimientos que aun cuando son infrecuentes en la patología infantil constituyen un grupo de alteraciones que hasta hace más de tres décadas eran irremediablemente fatales. Con el advenimiento del TCPH el pronóstico de estos síndromes ha mejorado sustancialmente, por lo que es importante conocer sus resultados, así como su morbimortalidad asociada.

Organogenesis of developing metanephroi allografted into nonimmunosuppressed adult rats / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To explore novel methods of possible donor organ supply and immunologic tolerance induction of organ transplantation.</p><p><b>METHODS</b>Whole metanephroi from d14-19 (E14-E19) embryos of pregnant rats were grouped and allografted into the omenta or near remnants of renal vessels of nonimmunosupressed adult rats. At the time of implantation, host rats underwent unilateral nephrectomy. Four weeks after implantation, allografted metanephroi in host rats were removed for gross, biochemical and histopathological examination.</p><p><b>RESULT</b>Four weeks post-implantation, (1) E19 and E18 metanephroi had enlarged,but were replaced by connective tissues. (2) E17 and E16 metanephroi showed the signs of acute rejection such as hypercellular glomeruli and lymphocyte infiltration in peritubular spaces. E16 grafted metanephroi underwent mild acute rejection of Banff schema, while E17 had moderate or severe acute rejection. When Cyclosporine A was administrated, E17 metanephroi formed mature nephrons and collecting ducts with few lymphocyte infiltration. (3) Metanephroi from E15 and E14 embryos allografted into the omentum or near remnants of renal vessels of uninephrectomized adult rats were enlarged and vascularized, and formed mature tubules and glomeruli. (4) The concentrations of urea nitrogen and creatinine in cyst fluid of E15 and E16 metanephroi were increased 40-fold and 50-fold, which were comparable to those in bladder urine. (5) In contrast, rat metanephroi did not grow or differentiate in rats without host kidney resection.</p><p><b>CONCLUSION</b>E14 and E15 metanephroi allografted into nonimmunosuppressed adult rats or E17 into cyclosporine-treated hosts undergo growth and differentiation and become vascularized. A variety of factors affect the growth and development of allografted metanephroi, while rejection is the main one.</p>

Cultured human embryonic neocortical cells survive and grow in infarcted cavities of adult rat brains and interconnect with host brain / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>There are no reports on exnografting cultured human fetal neocortical cells in this infracted cavities of adult rat brains. This study was undertaken to observe whether cultured human cortical neurons and astrocytes can survive and grow in the infarcted cavities of adult rat brains and whether they interconnect with host brains.</p><p><b>METHODS</b>The right middle cerebral artery was ligated distal to the striatal branches in 16 adult stroke-prone renovascular hypertensive rats. One week later, cultured cells from human embryonic cerebral cortexes were stereotaxically transferred to the infarcted cavity of 11 rats. The other 5 rats receiving sham transplants served as controls. For immunosuppression, all transplanted rats received intraperitoneal injection of cyclosporine A daily starting on the day of grafting. Immunohistochemistry for glial fibrillary acidic protein (GFAP), synaptophysin, neurofilament, and microtubule associated protein-2 (MAP-2) was performed on brain sections perfused in situ 8 weeks after transplantation.</p><p><b>RESULTS</b>Grafts in the infarcted cavities of 6 of 10 surviving rats consisted of bands of neurons with an immature appearance, bundles of fibers, and GFAP-immunopositive astrocytes, which were unevenly distributed. The grafts were rich in synaptophysin, neurofilament, and MAP2-positive neurons with long processes. The graft/host border was diffuse with dendrites apparently bridging over to the host brain, into which neurofilament immunopositive fibers protruded.</p><p><b>CONCLUSION</b>Cultured human fetal brain cells can survive and grow in the infarcted cavities of immunodepressed rats and integrate with the host brain.</p>

A bioética frente às situações emergentes: a esperança depositada em um cordão umbilical / Bioethics in Emerging Situations - Hope Deposited in an Umbilical Cord

A terapia celular carrega a promessa de se tornar a medicina do futuro, recuperando tecidos danificados ou mesmo eliminando a fila de transplantes. As células-tronco constituem um grupo celular que preserva a capacidade de auto-renovação, podendo diferenciar-se em tipos celulares diversos. A utilização de células-tronco de cordão umbilical, apesar de menos polêmica que as embrionárias, revela muitos aspectos de natureza moral e ética que emergem em relação aos bancos de armazenamento de uso autólogo e alogênico. Hoje, o uso da terapia celular ainda é bastante restrito e depende de um amplo desenvolvimento de pesquisas, contudo, uma grande esperança lhe é depositada. A Bioética é uma ferramenta para o delineamento de parâmetros éticos ao desenvolvimento tecno-científico. O presente estudo revelou que os limites e fronteiras de regulação de seu uso não estão claramente delineados e se mantém em constante transformação, percebendo-se uma clara tendência mundial no incentivo ao modelo alôgenico, a exemplo do Brasil e do Brasilcord. Em decorrência disso, o presente trabalho apresenta uma avaliação da utilização de células-tronco de cordão umbilical frente a visão da autonomia, da vulnerabilidade, da justiça, da equidade, da proteção, da precaução, da responsbilidade, da utilidade e da solidariedade.

Peritoneal transplantation of human fetal hepatocytes for the treatment of acute fatty liver of pregnancy: a case report.

We report the case of a 26-year-old second gravida in the third trimester of pregnancy who presented with a history of nausea, repeated vomiting and jaundice. The patient was diagnosed as acute fatty liver of pregnancy. After delivery, the condition of the patient progressed to grade IV encephalopathy and did not improve despite all intensive clinical management measures. After 3 days in grade IV encephalopathy, the patient was infused 3 x 10(8) human foetal hepatocytes. The patient's level of consciousness started improving after 24 hours of foetal hepatocyte transfusion and she recovered completely within 7 days.

Preliminary study on the development of germ cells from human fetal testicular tissues xenografted into the mouse / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the development of xenografted primitive human germ cells by using fetal testicular tissues as donor tissues and an immunodeficient mouse as the recipient.</p><p><b>METHODS</b>Testicular tissue fragments of a 26-week fetus were grafted under the back skin of a castrated immunodeficient mouse. Grafts were taken out after 135 days and processed for morphological and histological analyses.</p><p><b>RESULTS</b>The mass of grafts grew from about 1 mm in diameter and 5 mg in wet weight to about 3 mm and more than 20 mg 135 days after grafting. Histological observations showed a significant expansion of seminiferous tubules after grafting (80 +/- 25 microm in diameter) in comparison with seminiferous cords at the time of grafting (60 +/- 15 microm in diameter). The seminiferous cords developed into seminiferous tubules with the epithelial border and lumen. After 135 days of grafting, most of the dispersedly distributed primitive Sertoli cells and germ cells migrated to the basal part of seminiferous epithelium, located on the basement membrane and few of germ cells differentiated into spermatogonia.</p><p><b>CONCLUSION</b>Human fetal testicular tissues could survive and continuously develop after being xenograft into castrated immunodeficient mice.</p>

Expression of PCNA and Bcl-2 in the traumatic brains implanted with embryonic brain tissue in rats / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the expression of PCNA and Bcl-2 in the traumatic brain area transplanted with embryonic brain tissue in rats.</p><p><b>METHODS</b>The cerebral contusion of rats was induced by dropping weight. The homogenates of embryonic brain tissue were transplanted into the traumatic brain area two weeks after injury. All rats were sacrificed 6 weeks after injury (4 weeks after transplantation), and their brains were examined histologically. The expressions of PCNA and Bcl-2 in the brains were analyzed by immunohistochemical methods.</p><p><b>RESULTS</b>The histology of brain presented the capillary and glia proliferation, especially in the transplantation group. No significant difference was found in the expression of PCNA between two groups. However, Bcl-2 was overexpressed in the transplantation group.</p><p><b>CONCLUSION</b>The transplantation of the embryonic brain tissue enhances the expression of Bcl-2, which may play a neuroprotective role following traumatic brain injury.</p>

Evalution of the role of amniotic membrane transplantation in management of cicatricial entropion

This prospective study was carried out on 52 lids of 49 patients with cicatricial entropion. The lamellar split with vertical reposition of anterior lamella was the technique used in these cases, with an overall success of 98.1%. Good cosmetic results were obtained in all cases with a shorter period of healing with a mean of 15.73 +/- 2.63 days

Membrana amniótica no tratamento dos afinamentos corneais e esclerais / Amminiotic membrane in the treatment of corneal and scleral thinning

Objetivo: investigar a utilização da membrana amniótica como adjuvante no tratamento e restabelecimento de espessura dos afinamentos córneo-esclerais e epitelização corneal. Métodos: A membrana amniótica foi captada a partir de parto cesáreo e conservada em meio de preservação de córnea e glicerol 1:1 e conservada a - 80 graus centígrados. Sete olhos de 7 pacientes, sendo 4 portadores de afinamento corneal por afecções neurotróficas (Grupo 1: 2 herpes simples vírus; 1 após transplante de córnea; 1 por radioterapia) e 3 portadores de afinamento escleral após exerése de pterígio(Grupo 2: 2 com beta-terapia e 1 sem beta-terapia) foram submetidos à cirurgia para restabelecimento da superfície ocular e espessura córneo-escleral empregando membrana amniótica. Resultados: O tempo médio de seguimento foi de 12 meses (variação entre 11 meses e 15 meses). Um caso de úlcera neurotrófica secundária a radioterapia apresentou insucesso. Obtivemos sucesso do restabelecimento da superfície ocular e da espessura nos outros seis casos. Em relação à acuidade visual, 1 caso obteve melhora e os outros 6 permaneceram inalterados. A média de tempo de epitelização foi de 26,6 +/- 5,8 dias para o grupo 1 e 10,6 +/- 4,0 dias para o grupo 2. Conclusões: O uso de membrana amniótica constitui opção alternativa de grande utilidade na reconstrução da superfície ocular dos casos de afinamento córneo-escleral. Estudos com maior casuística e tempo de seguimento são necessários para melhor avaliar esse procedimento.

use of topical 5-flurouracil for primary pterygium surgery: comparison with amniotic membrane graft and conjunctival autograft

Purpose: To evaluate the efficacy and safety of intraoperative use of topical 5- fluorouracil in primary pterygium surgery and to compare the surgical outcome with amniotic membrane graft and conjunctival autograft. 86 eyes of 69 patients with primary pterygia were divided into 3 groups. Group 1 was treated with plerygium excision and conjunctival autogrft. Group 2 was treated with pterygium excision and amniotic membrane graft. Group 3 was treated with pterygium excision and intraoperative topical 5-flurouracil. The surgical outcome was compared in the treatment groups. Patients were followed for at least 6 months. The recurrence rate after conjunctival autograft, amniotic membrane graft, and topical 5-fluorouracil way 2.5%, 13.5%, and 13.3% respectively. There was no significant difference in recurrence rate among the three groups [P = 0.649] There was no major complications among the three groups. Conjunctival autograft, amniotic membrane graft, and topical 5- fluorouracil are equally effective in reducing the recurrence rate after primary pterygium excision