RESUMEN
Newcastle disease vaccines
Asunto(s)
Animales , Embrión de Pollo , Pollos/virología , Virus de la Enfermedad de Newcastle/patogenicidad , Enfermedades de las Aves de Corral/prevención & control , Vacunas Atenuadas/uso terapéutico , Vacunas Virales/uso terapéutico , Técnicas de Cultivo de Célula , Células Cultivadas , Pollos/inmunología , Virus de la Enfermedad de Newcastle/clasificación , Virus de la Enfermedad de Newcastle/crecimiento & desarrollo , Cultivo Primario de Células , Enfermedades de las Aves de Corral/inmunología , Enfermedades de las Aves de Corral/virología , Vacunación , Vacunas Atenuadas/efectos adversos , Vacunas Atenuadas/inmunología , Vacunas Virales/efectos adversos , Vacunas Virales/inmunologíaRESUMEN
Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2'-5'-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx) genes from duck. dTLR5 mRNA was highly expressed in the bursa of Fabricius, spleen, trachea, lung, jejunum, rectum, and skin; moderately expressed in the muscular and glandular tissues, duodenum, ileum, caecum, and pancreas; and minimally expressed in the heart, liver, kidney, and muscle. DF-1 or HeLa cells transfected with DNA constructs encoding dTLR5 can activate NF-kappaB leading to the activation of interleukin-6 (IL-6) promoter. When we challenged ducks with a Herts33 Newcastle disease virus (NDV), mRNA transcription of the antiviral molecules Mx, Double stranded RNA activated protein kinase (PKR), and OAS was up-regulated in the liver, lung, and spleen 1 and 2 days post-inoculation.