Effect of repeated exposure to isoflurane on the liver of rats fed on low protein diet Histopathological and histochemical study

Forty eight white rats divided into 8 equal groups were used. The animals of the first four groups were fed on a standard diet composed of milk and bread ad libitum. While the rats of the other four groups were kept on a special diet composed of bread in water, i.e. relatively protein poor diet ad libitum. Group I was exposed to O2 100%. Group II was exposed to 1% isoflurane in O2. Group III was exposed to hypoxic mixture [10% O2 in N2]. Group IV was exposed to 1% isoflurane carried by the hypoxic mixture. The animals of the last four groups kept on low protein diet were exposed in a similar sequence like the first 4 groups. Exposure was 30 minutes every other day for 12 exposures. No histopathological changes detected in rats exposed to O2 100%, fed on either types of food. While mild histopathological changes in the form of focal fatty change or vacular degeneration in other groups. Depressed activity of acid phosphatase, lactic dehydrogenase and succinic dehydrogenase enzymes were observed in groups maintained on low protein diet which was the probable cause for this depressed activity

Effect of repeated exposure to isoflurane on the liver of stressed rats Histopathological and histochemical study

Twenty four male white rats divided into 4 equal groups were used in this study. Group I was kept as a control. Group II was exposed to 1% isoflurane in oxygen 30 minutes every other day for 12 exposures. The rats of groups III and IV were subjected for 20 minutes of stress by hitting on their cages in non rhythmic way. Group III was exposed to 1% isoflurane in O2 for 12 exposures like group II. Liver sections stained with Hx and E and PAS showed no histopathological changes in groups I and III, focal fatty change in group II and vacular degeneration with ballooning of the hepatocytes in group IV. Mild depression of acid phosphatase, lactic and succinic dehydrogenase enzyme activity in stressed rats in groups III and V. These changes were mild and signify no pathological importance

Ascariasis: a surgical problem in children

Complications of ascariasis requiring surgical intervention have been frequently recorded in the literature, intestinal obstruction is the commonest complication, and could be produced by several mechanisms. In the majority of our cases, the obstruction was subacute and responded to conservative treatment. If the response is unsatisfactory, dispersal of the worms without opening the bowel should be tried firstly. But, there should be no hesitation to resect bowel if necessary. Perforation of the bowel is the most serious complication. It can occur in ulcerative lesions of the small intestine especially due to typhoid fever, meckel's and caecal diverticulitis with perforation and escape of ascaris into the pritoneal cavity have also been described [Van Rensburg and Kark, 1958]. It should be serious to perform intestinal resection in the presence of ascaris. For this reason, elective operation on the gastro-intestinal tract should be delayed until eradication of ascaris. We discussed our results in 28 children who presented with complications due to ascariasis

Histochemical enzymatic study of brain tumours

The distribution of 5 enzymes in 37 intracranial tumours was studied histochemically. The significance of the results was discussed. Generally there is decrease activity of the oxidative enzymes and increase activity of the hydrolytic enzymes in the malignant tumorus than in the bening ones