Résumé
OBJECTIVE: To determine polysaccharide in different compatibility of Fengshao liujunzi decoction.METHODS: Polysaccharide was extracted with water extracting-alcohol precipitation.Polysaccharide was prepared by anthrone-sulfuric acid method and determined by UV spectrophotometry.RESULTS: The optimal determination conditions for polysaccharide in Fengshao liujunzi decoction were as follows: 4 mL 0.2% anthrone-sulfuic acid solution was added into sample,heated in water bath for 15 min then cooled for 10 min.The linear range of D-glucosum anhydricum were 0.019 92～0.079 68 mg(r=0.999 6).The average recovery was 97.33%(RSD=1.52%,n=6).CONCLUSION: Polysaccharide is mainly from Liujunzi decoction.The method is rapid,accurate and reproducible for content determination of polysaccharide in different compatibility of Fengshao liujunzi decoction.
Résumé
OBJECTIVE: To establish the quality standards for Qudu capsules. METHODS: TLC was used to identify Rheum Palmatum, Phellodendron Amurense, Angelica Sinensis and Pheretima Aspergillum in Qudu capsules. HPLC was used to determine the content of the effective ingredient Paeonia Lactiflora. RESULTS: The spots characteristic of Rheum Palmatum, Phellodendron Amurense, Angelica Sinensis and Pheretima Aspergillum were found through detection by TLC. The linear range of Paeonia Lactiflora was 0. 094 4～ 0. 944 0? g( r=0. 999 8) . The average recovery was 99. 94% ( RSD=1. 74) . CONCLUSIONS: This standard can be used for the quality control of Qudu capsules.