Comparative study of the effecs of removing plaque by two toothbrushing methods / 北京大学学报(医学版)

Objective: To compare the effecte of removing plaque by two toothbrushing methods. Methods: Forty subjects were chosen to take part in the randomized、examiner-blind、crossover study. Results: Modified Bass method has better effect of removing plaque, especially that at the central margin than Vertical method(P

Expression of bone sialoprotein and osteopontin in developing dental tissues of rats / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the timing and location of the expression of bone sialoprotein (BSP) and osteopontin (OPN) in developing dental tissues of rats.</p><p><b>METHODS</b>Every three neonatal rats were sacrificed at day 1, weeks 1, 2, 3, 5 and 8. The mandibles were dissected and the first molar and the surrounding tissue were fixed, then demineralized with 15% EDTA. Immunohistochemical technique was used to determine the expression of BSP and OPN in dental tissues and the surrounding bone at different time points.</p><p><b>RESULTS</b>Immunoreactivitis of BSP and OPN were present in matured ameloblasts, dentinoblasts, cementoblasts, osteoblasts, and the matrix. The expression of BSP and OPN in cementum and alveolar bone was stronger than that in enamel and dentine. In cementum and alveolar bone, BSP appeared to be concentrated in unmineralized and mineralized tissues, but OPN was concentrated in the mineralizing frontier and reversal line.</p><p><b>CONCLUSIONS</b>BSP and OPN play an important role in the development and mineralization of rat mineralized tissues. The expression of BSP was different from OPN, indicating their different functions.</p>

Analysis of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid and its clinical significance / 中华口腔医学杂志

<p><b>OBJECTIVES</b>To study the essence of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid (GCF).</p><p><b>METHODS</b>GCF samples from patients with RPP and AP were collected. 12 000-protein was separated by SDS-PAGE and transformed to PVDF by electronic transformation. The aim band was cut to be analyzed in 491 Protein Sequencer.</p><p><b>RESULTS</b>The first ten of N-terminal amino acid sequence of 12 000-protein in GCF was Met, Leu, Thr, Glu, Leu, Glu, Lys, Ala, Leu, Asn. Through checking up in MS-Edman, the sequence was similar to "Ca binding protein, MRP8" which is the light subunit of Calprotectin.</p><p><b>CONCLUSIONS</b>Calprotectin is a major protein in granulocytes and monocytes, and is related to many inflammatory diseases, maybe served as a effective marker for evaluating the inflammation of periodontium.</p>

Effects of non-surgical treatment modalities on peri-implantitis / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the effects of local-delivery of 25% metronidazol gel and mechanical cleaning using ultrasonic carbon fiber tip on dental implants with peri-implantitis.</p><p><b>METHODS</b>27 implants with peri-implantitis were randomly assigned to receiving either 25% metronidazol gel treatment or carbon fiber tip ultrasonic scaling. All parameters including plaque index (PLI), probing depth (PD) of pocket, sulcular bleeding index (SBI), and BANA enzyme analysis were measured at baseline, 1, 2, 6 and 12 weeks after treatment.</p><p><b>RESULTS</b>Statistically significant decrease (P < 0.05) in SBI, BANA test and PLI occurred in both treatment groups at all time intervals compared to baseline. PD had a decreasing tendency in both groups, but only metronidazole group reached statistically significant level (P < 0.05) at 2 and 6 week intervals compared to baseline. None of the treatment modalities produced any side effects on the implant and peri-implant tissues.</p><p><b>CONCLUSIONS</b>Both 25% metronidazol gel and mechanical cleaning using ultrasonic carbon fiber tip can be safely and effectively used in the treatment of peri-implant diseases.</p>

A longitudinal study of 5 cephalometric plane angles in female children from 7 to 12 years old / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this study was to find the laws of angular changes of 5 cephalometric planes in children with mixed dentitions.</p><p><b>METHODS</b>Nineteen female children were annually examined with X-ray, and the cephalometric analysis was performed in continuous 4 years. Of these children, 10 children were investigated from the age of 7 years old, and the other 9 children from 9 years old. The two groups were longitudinally studied. All of the children were observed almost at the same age (9-10 years old) in the two groups, and the results were analyzed with mixed longitudinal methods. All data were analyzed with SPSS 8.0 software.</p><p><b>RESULTS</b>In the observation period, the angular relationship of FH to BaN remain constant among 5 cephalometric planes, and FH to SN was almost in the same situation.</p><p><b>CONCLUSION</b>In mixed dentition, the angular relationship of FH, BaN and SN barely change.</p>

A longitudinal study of vertical growth of craniofacial complex in female children with normal occlusion from 7 to 12 years old / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To find the laws of vertical growth of craniofacial complex in children with mixed dentition.</p><p><b>METHODS</b>19 female children were annually observed with cephalometric film in 4 successive years, of whom 10 started at 7 years while the others at age of 9. The two groups were longitudinally studied. All data were analyzed by SPSS 8.0 software.</p><p><b>RESULTS</b>In the observation period, the height of most craniofacial regions continually grew. In anterior face, the middle facial height increased most (increased 4.7 mm from 7 to 10 years old, P < 0.01; increased 3.9 mm from 10 to 12 years old, P < 0.01), while the lower facial height nearly unchanged (increased 1.3 mm from 7 to 10 years old, P > 0.05; increased 1.4 mm from 10 to 12 years old, P > 0.05). Some other regions had their own growing characters.</p><p><b>CONCLUSIONS</b>Once the occlusal relationships of permanent incisors are established, it is difficult to control the anterior lower facial height with orthodontic treatments.</p>

Isolation, culture and identification of bovine cementum-derived cells / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To isolate and culture bovine cementum-derived cells and investigate their biological properties.</p><p><b>METHODS</b>Culture of primary bovine cementoblast (CB) were established from new-born bovine teeth. Cementum was manually dissected, fragmented, and digested twice with collagenase. Following a thorough wash to remove liberated cells, the remaining cementum fragments were plated and cultured in RPMI1640 containing 10% FCS and 1.5% Hepes. The cells in culture were identified using immunocytochemistry by expression of cementum attachment protein (CAP), osteocalcin (OCN) and alkaline phosphatase (ALP). ALP activity was measured by modified Gomori staining.</p><p><b>RESULTS</b>(1) The cells in culture possessed the typical morphology of CB and there were no obvious change of the cell morphology up to 5th passage; (2) Cells in culture exhibited alkaline phosphatase activity and were also positive to CAP, OPN and ALP immunohistochemistry staining.</p><p><b>CONCLUSION</b>The cells isolated and cultured in this experiment possess the properties of CB. This enables us to further observe its biological characteristics during cementogenesis and periodontal regeneration.</p>

A cephalometric study of the mandible in female children from 7 to 12 years old / 实用口腔医学杂志

?Objective:To investigate the mandibular growth in children with mixed dentition. Methods:Nineteen female children were yearly observed with cephalometric film in 4 successive years.The study began in 10 children at 7 years old and in another 9 at 9 years old. There was the same observation period (9 10 years old) in the two groups, therefore their results could be combined to analysis with mixed longitudinal methods. All data were analysed with SPSS 8.0 software.Results:Generally,Ar Po and Go Po increased significantly ( P 0.05 ).Conclusion:In mixed dentition, the mandibular depth growth is greater than its height growth in children with normal face.