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1.
Braz. j. med. biol. res ; 48(12): 1115-1121, Dec. 2015. graf
Article Dans Anglais | LILACS | ID: lil-762912

Résumé

The levels of serum inflammatory cytokines and the activation of nuclear factor kappa B (NF-κB) and hypoxia inducible factor-1α (HIF-1α) in heart tissues in response to different frequencies of intermittent hypoxia (IH) and the antioxidant tempol were evaluated. Wistar rats (64 males, 200-220 g) were randomly divided into 6 experimental groups and 2 control groups. Four groups were exposed to IH 10, 20, 30, or 40 times/h. The other 2 experimental groups were challenged with IH (30 times/h) plus tempol, either beginning on day 0 (IH30T0) or on day 29 (IH30T29). After 6 weeks of challenge, serum levels of tumor necrosis factor (TNF)-α, intracellular adhesion molecule (ICAM)-1, and interleukin-10 were measured, and western blot analysis was used to detect NF-κB p65 and HIF-1α in myocardial tissues. Serum levels of TNF-α and ICAM-1 and myocardial expression of NF-κB p65 and HIF-1α were all significantly higher in IH rats than in controls (P<0.001). Increased IH frequency resulted in more significant changes. Administration of tempol in IH rats significantly reduced levels of TNF-α, ICAM-1, NF-κB and HIF-1α compared with the non-tempol-treated group (F=16.936, P<0.001). IH induced an inflammatory response in a frequency-dependent manner. Additionally, HIF-1α and NF-κB were increased following IH administration. Importantly, tempol treatment attenuated this effect.


Sujets)
Animaux , Mâle , Hypoxie/complications , Antioxydants/administration et posologie , N-oxydes cycliques/administration et posologie , Inflammation/prévention et contrôle , Hypoxie/sang , Gazométrie sanguine , Technique de Western , Test ELISA , Sous-unité alpha du facteur-1 induit par l'hypoxie/analyse , Inflammation/métabolisme , Molécule-1 d'adhérence intercellulaire/sang , /sang , Myocarde/métabolisme , Myocarde/anatomopathologie , Facteur de transcription NF-kappa B/analyse , Rat Wistar , Marqueurs de spin , Facteur de nécrose tumorale alpha/sang
2.
Braz. j. med. biol. res ; 46(9): 758-764, 19/set. 2013. graf
Article Dans Anglais | LILACS | ID: lil-686576

Résumé

To explore the effects of adipose tissue-derived stem cells (ADSCs) on the proliferation and invasion of pancreatic cancer cells in vitro and the possible mechanism involved, ADSCs were cocultured with pancreatic cancer cells, and a cell counting kit (CCK-8) was used to detect the proliferation of pancreatic cancer cells. ELISA was used to determine the concentration of stromal cell-derived factor-1 (SDF-1) in the supernatants. RT-PCR was performed to detect the expression of the chemokine receptor CXCR4 in pancreatic cancer cells and ADSCs. An in vitro invasion assay was used to measure invasion of pancreatic cancer cells. SDF-1 was detected in the supernatants of ADSCs, but not in pancreatic cancer cells. Higher CXCR4 mRNA levels were detected in the pancreatic cancer cell lines compared with ADSCs (109.3±10.7 and 97.6±7.6 vs 18.3±1.7, respectively; P<0.01). In addition, conditioned medium from ADSCs promoted the proliferation and invasion of pancreatic cancer cells, and AMD3100, a CXCR4 antagonist, significantly downregulated these growth-promoting effects. We conclude that ADSCs can promote the proliferation and invasion of pancreatic cancer cells, which may involve the SDF-1/CXCR4 axis.


Sujets)
Humains , Tissu adipeux/anatomopathologie , Prolifération cellulaire , /analyse , Tumeurs du pancréas/anatomopathologie , /analyse , Cellules souches/physiologie , Adipocytes/cytologie , Différenciation cellulaire , Lignée cellulaire tumorale , Techniques de coculture , Milieux de culture conditionnés , Test ELISA , Invasion tumorale/physiopathologie , Tumeurs du pancréas/métabolisme , Réaction de polymérisation en chaine en temps réel , ARN messager/métabolisme , /génétique , /métabolisme , Cellules souches/anatomopathologie
3.
Southeast Asian J Trop Med Public Health ; 1997 Jun; 28(2): 268-73
Article Dans Anglais | IMSEAR | ID: sea-36005

Résumé

The present study was designed to explore if there exists a correlation between predominant isotype-defined antibody levels and reinfection in low age groups of the population in an endemic area of schistosomiasis japonica in China. One hundred and thirty-eight individuals aged 3-25 years old were selected for serological investigations including the levels of IgG, IgG4, IgM and IgE, detected by ELISA with soluble egg antigen and soluble adult worm antigen. Results show that age is a determinant for SEA-specific IgG, IgG4, and IgE, and SWA-specific IgG and IgG4 antibody levels, which increased with age, and that SEA- and SWA- specific IgG4 antibody levels are risk factors of reinfection, ie, the risk of reinfection occurrence of the population with high level of SEA or SWA-specific IgG4 is 2.83 or 2.40 times, respectively, that with low level of SEA or SWA-specific IgG4, suggesting that in the endemic area of schistosomiasis japonica, there exists a possibility that in the population aged 3-25 years, SEA and SWA-specific IgG4 antibodies mediate a blocking immunity response.


Sujets)
Adolescent , Adulte , Anticorps antihelminthe/sang , Enfant , Enfant d'âge préscolaire , Chine/épidémiologie , Prédisposition aux maladies , Test ELISA , Femelle , Humains , Isotypes des immunoglobulines/sang , Modèles logistiques , Mâle , Odds ratio , Prévalence , Facteurs de risque , Schistosomiase artérioveineuse/épidémiologie , Répartition par sexe
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