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OBJECTIVE To set up a quick method to detect pathogens in cerebrospinal fluid.METHODS The method of polymerase chain reaction(PCR),using a pair of universal primers targeted at the 16S rRNA gene,was adopted to amplify the DNA of bacterium.138 clinical specimens obtained from patients were examined by PCR method and bacterial culture method.RESULTS The positive rate was 39.86% of PCR and 17.39% of culture,the outcomes had statistical significance(P
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OBJECTIVE To explore study a method for rapid detection of bacterial infection in clinic to diagnose septicemia early.METHODS 16S rRNA gene of ten bacterial species was amplified with PCR,by using human genome DNA,HBV-DNA and Candida albicans as comparison.The sensitivity test was done by the method of gradual dilution of Escherichia coli.RESULTS The bacterial species were amplified and the products were 371 bp,but human genome DNA,HBV-DNA and C.albicans showed no amplification products.Sensitivity test showed that it could detect as low as 1.5?104/L of E.coli.CONCLUSIONS The method is rapid and highly specific and sensitive in detecting the existence of bacterial 16S rRNA gene.
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OBJECTIVE To investigate drug resistance characteristice of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum?-lactamases(ESBLs) in our hospital so as to instruct rational use of antibiotics in clinic. METHODS The ESBLs were determined by the sensitivity and confirming tests according to NCCLS(1999) standard. RESULTS The isolated ratio of ESBLs producing E. coli and K. pneumoniae increased from 28.6% and 29.2% in 2005 to 48.5% and 49.2% in 2007. Extended-Spectum ?-Lactamases germs showed high resistance to cephalosporins-1G, 2G, 3G, to Penicillin and to Am Aztreonam. The rate of sensitivity to imipenem was 100.0%. CONCLUSIONS There is an increasing trend of ESBLs producing E. coli and K. pneumoniae. The imipenem is the best drug for treatment the bacterium producing ESBLs.