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1.
China Journal of Chinese Materia Medica ; (24): 744-746, 2006.
Article Dans Chinois | WPRIM | ID: wpr-351766

Résumé

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.</p><p><b>METHOD</b>C18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.</p><p><b>RESULT</b>Lower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.</p><p><b>CONCLUSION</b>A simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.</p>


Sujets)
Chromatographie en phase liquide à haute performance , Méthodes , Lobelia , Chimie , Structure moléculaire , Plantes médicinales , Chimie , Polymère de polyacétylène , Chimie , Contrôle de qualité , Reproductibilité des résultats
2.
China Journal of Chinese Materia Medica ; (24): 706-710, 2003.
Article Dans Chinois | WPRIM | ID: wpr-282262

Résumé

<p><b>OBJECTIVE</b>To find the patterns of the rDNA ITS sequence variation of Schisandra sphenanthera and S. viridis, and to establish the molecular biological method for the identification of Fructus Schisandrae Sphenantherae and the fruits of S. viridis.</p><p><b>METHOD</b>PCR products were sequenced directly and the sequences were analyzed with PAUP 4.0b10. NJ systematic tree was obtained with neighbor-joining method.</p><p><b>RESULT</b>The Complete ITS sequence of S. sphenanthera was 691-692 bp, of which there were 282 bp of ITS1 and 246-247 bp of ITS2. The complete sequence of S. viridis was 694-695 bp, consisting of 285-286 bp of ITS1 and 246-247 bp of ITS2. There were three informative sites in ITS1 regions for the two species. In the NJ tree with Kadsura anamosma and K. coccinea as outgroups, five different populations of S. viridis were the monophyletic group with the bootstrap value of 68%. These populations included one from Tianmushan, Zhejiang province, three populations from Jigongshan, Henan Province and the other two populations of S. viridis cited the sequences from GeneBank (registration numbers are AF263438 and AF163703 respectively).</p><p><b>CONCLUSION</b>The rDNA internal transcribed spacer is a good marker to distinguish the Fructus Schisandrae Sphenantherae from the fruits of S. viridis.</p>


Sujets)
Séquence nucléotidique , ADN des plantes , Génétique , Espaceur de l'ADN ribosomique , Génétique , Contamination de médicament , Fruit , Génétique , Données de séquences moléculaires , Phylogenèse , Plantes médicinales , Classification , Génétique , Génétique , Schisandra , Classification , Génétique , Analyse de séquence d'ADN
3.
China Journal of Chinese Materia Medica ; (24): 123-125, 2002.
Article Dans Chinois | WPRIM | ID: wpr-274999

Résumé

<p><b>OBJECTIVE</b>To study the chemical constituents of Costus speciosus and C. tonkinensis (Zingiberaceae) distributed in Yunnan province.</p><p><b>METHOD</b>Chromatography and spectral analyses were used to isolate the constituents and elucidate their structure.</p><p><b>RESULT</b>Six compounds were isolated from the rhizome of C. speciosus and elucidated as diosgenin(1), prosapogenin B of dioscin(2), diosgenone(3), cycloartanol(4), 25-en-cycloartenol(5) and octacosanoic acid(6). Four compounds were isolated from the rhizome of Costus tonkinensis and elucidated as tetracosanoic acid(7), succinic acid(8), beta-sitosterol(9) and daucosterin(10).</p><p><b>CONCLUSION</b>Compounds of 3-6 were obtained from C. speciosus for the first time and compounds of 7-10 were obtained from C. tonkinensis for the first time too.</p>


Sujets)
Costus , Chimie , Classification , Acides gras , Chimie , Plantes médicinales , Chimie , Rhizome , Chimie , Spiranes , Chimie , Triterpènes , Chimie
4.
China Journal of Chinese Materia Medica ; (24): 130-131, 2002.
Article Dans Chinois | WPRIM | ID: wpr-274996

Résumé

<p><b>OBJECTIVE</b>To study the chemical constituents of Alpinia jianganfeng (Zingiberaceae) distributed in Sichuan province.</p><p><b>METHOD</b>Chromatography and spectral analyses were used to isolate the constituents and elucidate their structure.</p><p><b>RESULT</b>Four compounds were isolated from the rhizome of A. jianganfeng and elucidated as kaempferol-3-O-glucuronide(1), docosanoic acid(2), 3-hydroxy-stigmast-5-en-7-one(3) and beta-sitosterol(4).</p><p><b>CONCLUSION</b>All these compounds were obtained from this plant for the first time.</p>


Sujets)
Alpinia , Chimie , Acides gras , Chimie , Plantes médicinales , Chimie , Rhizome , Chimie , Sitostérol , Chimie
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