MSX 1 gene variant and non-syndromic clefting: Association or rejection.

Context: Non-syndromic cleft lip/palate (NSCL/P) is a congenital anomaly with significant medical, psychological and social ramifications. There is sufficient evidence to hypothesize that locus for this condition can be identified by candidate genes. Aims: The aim of this study is to amplify the chosen region (799 G >T) of MSX 1 gene, investigate the degree of association and perform a mutation research from Raichur cleft lip and palate patient sample. Settings and Design: Case history and clinical examination of the patient were recorded to rule. Written consent was obtained from patients and controls for in vivo study. Study was designed in four steps as follows: Collection of a blood sample Genomic deoxyribonucleic acid (DNA) extraction Polymerase chain reaction (PCR) Restriction fragment length polymorphism (RFLP). Materials and Methods: Blood samples were collected from 50 subjects having NSCL/P and 50 controls. Genomic DNA was extracted, PCR and RFLP was performed for digestion products that were evaluated. Statistical Analysis: Chi-square test with P value at 95% confidence intervals. Results: The results showed a positive correlation between MSX 1 799 G >T gene variant and NSCL/P patients in Raichur patients. Conclusions: From a genetically diverse etiology MSX 1 799 G >T gene variant may be a good screening marker for NSCL/P in Raichur patients.

Msx1 Gene Variant - its Association in Isolated Hypodontia: A Case Control Genetic study!!!.

INTRODUCTION: Non‑syndromic tooth agenesis is a congenital anomaly with significant medical, psychological, and social ramifications. There is sufficient evidence to hypothesize that locus for this condition can be identified by candidate genes. AIM OF THE STUDY: The aim of this study was to test whether MSX1 671 T > C gene variant was involved in etiology of non‑syndromic tooth agenesis in Raichur patients. MATERIALS AND METHODS: Blood samples were collected with informed consent from 50 subjects having non‑syndromic tooth agenesis and 50 controls. Genomic deoxyribonucleic acid (DNA) was extracted from the blood samples, polymerase chain reaction (PCR) was performed, and restriction fragment length polymorphism (RFLP) was performed for digestion products that were evaluated. RESULTS: The results showed positive correlation between MSX1671 T > C gene variant and non‑syndromic tooth agenesis in Raichur patients. CONCLUSION: MSX1 671 T > C gene variant may be a good screening marker for non‑syndromic tooth agenesis in Raichur patients.

Crystal growth vs. conventional acid etching: a comparative evaluation of etch patterns, penetration depths, and bond strengths.

The present study was undertaken to investigate the effect on enamel surface, penetration depth, and bond strength produced by 37% phosphoric acid and 20% sulfated polyacrylic acid as etching agents for direct bonding. Eighty teeth were used to study the efficacy of the etching agents on the enamel surface, penetration depth, and tensile bond strength. It was determined from the present study that a 30 sec application of 20% sulfated polyacrylic acid produced comparable etching topography with that of 37% phosphoric acid applied for 30 sec. The 37% phosphoric acid dissolves enamel to a greater extent than does the 20% sulfated polyacrylic acid. Instron Universal testing machine was used to evaluate the bond strengths of the two etching agents. Twenty percent sulfated polyacrylic acid provided adequate tensile bond strength. It was ascertained that crystal growth can be an alternative to conventional phosphoric acid etching as it dissolves lesser enamel and provides adequate tensile bond strength.