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Braz. j. microbiol ; 32(1): 56-60, Jan.-Mar. 2001. ilus, tab, graf
Article Dans Anglais | LILACS | ID: lil-297668

Résumé

Two strains of Aspergillus flavus, non-toxigenic NRRL 6550 and toxigenic NRRL 5940, were studied over a period of 44 days, in order to detect the presence of virus-like particles (VLPs) by means of electron microscopy (EM) and nucleic acids electrophoresis. Only the toxigenic strain contained VLPs, presenting three-segmented dsRNA. An increase in VLPs number was observed during the exponential phase of fungal growth, up to day 12 of culture; after this, higher levels of aflatoxin production in toxigenic NRRL 5940 mycelia occurred in parallel with decreased VLPs replication.


Sujets)
Aspergillus flavus , Techniques in vitro , ARN , Virus à ARN , Électrophorèse , Microscopie électronique , Culture virale
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