Résumé
Aims: This study was designed to evaluate the antidiabetic effects of aqueous and ethanolic extracts of Trichilia emetica(TE) stem bark in albinos rats.Methodology:24 rats of comparable weight were divided into 2 lots, including a control lot of 3 rats and a test lot of 21 rats. Diabetes was induced by injecting Alloxan® intraperitoneally (125 mg/kg bw) for 7 days. After the onset of diabetes, the animals were divided into several groups and given the extracts of Trichilia emeticaand Diastabol®orally for 6 days according to the following protocol: Normal Control (NC) received normal saline, group diabetic control (DC) it consists of untreated diabetic, group DD10 and DD20 was comprised of diabetic animals treated with Diastabol® at a doses of 10 and 20 mg/kg bw respectively , group DTEE100 and DTEE200 it consists of diabetic animals treated with ethanolic extract at a doses of 100 and 200 mg/kg bw respectively,group DTEA100 and DDTEA200 was comprised of diabetic animals treated with aqueous extract at a same dose (100 and 200 mg/kg bw). At the end of experimentation, some blood was collected for the determination of some biochemical parameters such as insulin, blood glucose, AST, ALT, urea and creatinine. Systolic blood pressure (SBP), diastolic blood pressure (DBP), cardiac frequency (CF) were also recorded. Results:The results showed a significant (P<0.05) decrease in insulin levelsin diabetic rats compared with NC. In addition, diabetes caused a significant increase (P<0.05) in blood glucose, urea, creatinine levels,transaminase activity, and in blood pressure numbers (DBP, SBP and CF) still in comparison with NC. However, the treatment of sick animals with the extracts and the Diastabol at the doses listed above significantly (P <0.05)increased insulin levels and reduced the sames biochemical parameters levels as well as the blood pressure numberscompared with DC.Conclusion:The data obtained showed that this part of the plant would have antidiabetic effects with ethanolic extract at the dose of 200 mg/kg bw had the highest pronounced effect and could be used as a good alternative for diabetes management, thus justifying its use in traditional medicine
Résumé
Aims: Gentamicin, a widely used aminoglycoside antibiotic, is recognized as possessingsignificant nephrotoxic potential in human beings. The aim of this study was to determine theprotective effect of aqueous and hydroethanolic extracts of Crinum scillifoliumon gentamicininduced nephrotoxicity using biochemical approaches and determined the most active extractin rat Study Design: The leaves of Crinumscillifoliumwere collected in the district of Me(Côte d’Ivoire). The plant was identified and authenticated by the Department of Botany, Felix Houphouet Boigny University of Abidjan (Côte d’Ivoire).Place and Duration of Study:Analysis of the plant samples was done in pharmacodynamicsBiochemistry Laboratory, Felix Houphouet Boigny University and the Laboratory of Organic Chemistry and Natural Substances, Felix Houphouet Boigny University between July and August 2018.Methodology: The extractions were execcuted by macerating 100 g of plant dry powder in 1liter of distilled water (aqueous extraction) or in 2 liters of water / ethanol mixture (30/70 V /(ethanol extraction). The animals were divided according to weight in seven groups each of six rats. The nephroprotective effects were estimated by comparing the effects of the extracts (100 and 200mg / kg) to that of vitamin E (250 mg / kg) against gentamicin-induced renal failure by (80 mg / kg). Gentamicin is administered to the animals one hour after treatment with the extracts for seven days.Results:The administration of gentamicin through intraperitoneal route to rats for seven days,resulted in an increase in urea and creatinine concentrations as well as decrease of total protein concentration in the serum. The level of total protein and albumin increased in urine after administration of gentamicin. The treatment of animals suffering from nephrotoxicity with Hydroethanolic and aqueous extracts of Crinum scillifoliumwould have significantly reduced(P < 0.001 and P < 0.01) biochemical parameters considered as markers of nephrotoxicity.Conclusion: This study proved that the aqueous extract of Crinum scillifoliumpossesses a nephroprotective activity against gentamicin-induced kidney failure in rats. So aqueous extract can beuseful for preventives applications
Résumé
Among the HIV / AIDS infected, individuals contract fungal infections of which many die as a direct consequence of these infections. In this study, the antifungal activity of ten extracts (stem bark) from Terminalia mantaly H. Perrier was evaluated on the in vitro growth of clinical isolate of pathogenic fungi (Cryptococcus neoformans). Agar double dilution method in slope tubes was adopted to determine anticryptococcal activity. All extracts exhibited antifungal activity in dose-response relationship. The residue extracts T4-2 obtained after degreasing the hydro-alcoholic extract T0 (MFC = 24.37μg/Ml; IC50 = 5.87μg/mL) is the most active extract. Moreover, for a given concentration it is said that there are not significant differences between the different tests for each extract (P< 0.05). Therefore, using the hydro-alcoholic solvent (70% ethanol) followed by removal of oil is the best way to obtain an optimally concentrated active ingredient from Terminalia mantaly. The present study justifies the traditional use of this plant for the treatment of fungal infections.