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Article Dans Anglais | LILACS | ID: lil-724683

Résumé

Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasite’s DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasite’s DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.


Sujets)
Animaux , Diagnostic , Faux négatifs , Leishmaniose/anatomopathologie , Réaction de polymérisation en chaîne
2.
J. venom. anim. toxins incl. trop. dis ; 20: 1-6, 04/02/2014. tab, ilus
Article Dans Anglais | LILACS, VETINDEX | ID: biblio-1484572

Résumé

Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasite’s DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasite’s DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.


Sujets)
Animaux , Diagnostic , Leishmaniose/anatomopathologie , Réaction de polymérisation en chaîne , Faux négatifs
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