Résumé
We isolated a 4301-bp cDNA from a human foetal brain cDNA library by high-throughput cDNA sequencing. It encodes a protein of 341 amino acids, which shows 69% identity with the human kinase CLIK1 (AAL99353), which was suggested to be the CLP-36 interacting kinase. Bioinformatics analysis suggests that the putative kinase may interact with PDZ and LIM domain proteins. Therefore the protein and its cDNA were named 'PDLIM1 interacting kinase 1 like' (PDIK1L; nomenclature approved by the HUGO Gene Nomenclature Committee). Ensembl Genome Browser located PDIK1L to human chromosome 1p35.3. It spans about 13.7 kb and consists of four exons and three introns. Multiple-tissue cDNA panel PCR revealed that the gene is expressed widely in human tissues: liver, kidney, pancreas, spleen, thymus and prostate. The protein appears to be localized to the nucleus.
Sujets)
Séquence d'acides aminés , Séquence nucléotidique , Encéphale/physiologie , Clonage moléculaire , ADN complémentaire , Protéines de liaison à l'ADN/génétique , Expression des gènes , Humains , Données de séquences moléculaires , Protéines de tissu nerveux/génétique , Protéines nucléaires , Phosphotransferases/génétique , Réaction de polymérisation en chaîne , Protein kinases , Protein-Serine-Threonine Kinases/génétique , Similitude de séquences d'acides aminés , Fractions subcellulaires , Distribution tissulaire , Facteurs de transcriptionRésumé
We report cloning and some features of a novel human gene, MATH2, which encodes a protein of 337 amino acid residues with a basic helix loop helix domain and exhibits 98% similarity to mouse Math2. Results of Northern blot analysis revealed two transcripts of the MATH2 gene of 1.7 kb and 2.4 kb in human brain. We localized MATH2 to chromosome 7 at 7p14-15 by matching with the Human Genome Sequence Database. Human MATH2 and mouse Math2 may have the same functions in the nervous system.