Résumé
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema.</p><p><b>METHOD</b>The methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75:25:0.01) at flow-rate 1.0 mL x min(-1). The drift-tube temperature of ELSD was 85 degrees C, and the flow rate of air was 3.0 L x min(-1).</p><p><b>RESULT</b>Hyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear (r = 0.9998) over the range of 2.4-24 microg. The average recovery was 97.1% (RSD 2.9%, n=5).</p><p><b>CONCLUSION</b>The method could be used for quality control of Bile Arisaema.</p>
Sujets)
Animaux , Arisaema , Chimie , Bile , Chimie , Chromatographie en phase liquide à haute performance , Méthodes , Acide désoxycholique , Association médicamenteuse , Température élevée , Lumière , Médecine traditionnelle chinoise , Normes de référence , Tubercules , Chimie , Plantes médicinales , Chimie , Contrôle de qualité , Reproductibilité des résultats , Diffusion de rayonnementsRésumé
<p><b>OBJECTIVE</b>To explore the utility of Principal Factor Analysis (PFA) in chromatographic data for quality control.</p><p><b>METHOD</b>Chromatographic fingerprints of processed root pieces of Paeonia lactiflora were determined by HPLC, the PFA was used for data processing.</p><p><b>RESULT</b>The quantitative differences among different growing areas and different processing batches were found with the method.</p><p><b>CONCLUSION</b>The method could be used in quality control for monitoring between-batch products of traditional Chinese pharmaceutical process.</p>
Sujets)
Chromatographie en phase liquide à haute performance , Méthodes , Écosystème , Analyse statistique factorielle , Température élevée , Pinellia , Chimie , Préparations à base de plantes , Chimie , Normes de référence , Racines de plante , Chimie , Plantes médicinales , Chimie , Contrôle de qualité , Reproductibilité des résultats , Technologie pharmaceutique , Méthodes , VinRésumé
<p><b>OBJECTIVE</b>To provide the experimental data for the quality control of Paeonia lactiflora a prepared Chinese medicinal herb.</p><p><b>METHOD</b>The contents paeoniflorin among different growing areas and different processing methods methods were assayed by HPLC.</p><p><b>RESULT</b>The quantitative differences of paeoniflorin content among different growing areas and different processing methods wereanalyzed quantitatively.</p><p><b>CONCLUSION</b>The quantitative differences among different growing areas were not significant and the quantitative differences among different processing methods within 10%.</p>
Sujets)
Benzoates , Composés pontés , Chine , Chromatographie en phase liquide à haute performance , Écosystème , Glucosides , Température élevée , Monoterpènes , Paeonia , Chimie , Racines de plante , Chimie , Plantes médicinales , Chimie , Contrôle de qualité , Technologie pharmaceutique , Méthodes , VinRésumé
<p><b>OBJECTIVE</b>To provide experimental data for the quality control of processed Paeonia lactiflora, a Chinese herbal medicine.</p><p><b>METHOD</b>Traditional processing of P. lactiflora was simulated, content of paeoniflorin and water extracts among different preparations were assayed by HPLC; The quantitative correlations among different processing conditions were analyzed, the effects of processing parameters on the contents of paeoniflorin and water extracts were assayed a nanalysed.</p><p><b>RESULT AND CONCLUSION</b>The controlled processing parameters were correlated with covariables which showed that processing procedures was controllable, and the heating temperature was a factor impacting the content of paeoniflorin.</p>