Résumé
Objective To study the situation of HIV infections among men who have sex with men (MSM) in Tianjin during 2008-2009 and to provide reasonable evidence for intervention strategy.Methods Transect investigations in MSM were conducted three times during 2008-2009.Blood samples were collected and detected to identify the recent HIV infection with IgG-capture BED-enzyme immunoassay (BED-CEIA) before HIV incidence was estimated.Results 1799 specimens were tested and the HIV prevalence rates of each study were 6.7％,8.6％ and 6.2％,while the incidence rates were 2.7％,2.5％ and 2.8％,respectively.The estimated incidence rates among these testees were 5.36％ and 5.52％ per year in 2008 and 2009.Conclusion Results of this study showed that the HIV incidence stabilized at high level among MSM in Tianjin,calling for the effective interventions be taken for HIV/AIDS control and prevention.
Résumé
Different-length fragments of the HIV trans-membrane antigen (gp120) gene were expressed in Escherichia coli, using T7 expression system. SDS-PAGE stained by Coomassic Brilliant Blue showed no expression of full-length gp120 and poor expression of half-length gp120 fragments from the N-terminal, but high expression of 1/3-lgenth gp120 gene fragments from the N-terminal (including V1/V2 epitopes), more than 18% of total bacterial protein. Western blot showed fairly good reactivity to serum from HIV-infected individual. On this basis, we expressed the corresponding tp 120 fragments of the HIV strains epidemic in China. This study laid a solid foundation for exploration of high expression of gp 120 in E.coli and development of HIV serological diagnostic system for Chinese people.