Résumé
<p><b>OBJECTIVE</b>To explore a new method for the therapy of avascular necrosis of the femoral head.</p><p><b>METHOD</b>The recombinant plasmid pCD-rbFGF was mixed with collagen and was implanted in the necrotic femoral head. Expression of basic fibroblast growth factor (bFGF) was examined by RT-PCR and immunohistochemical method. Repair of the femoral head was observed by histological and histomorphometric analysis.</p><p><b>RESULT</b>Expression of bFGF was detected in the femoral head transfected with bFGF gene, indicating significant increase of angiogenesis 2 weeks after gene transfection and increased new bone formation 8 weeks after gene transfection on histomorphometric analysis (P< 0.01).</p><p><b>CONCLUSION</b>Transfection of bFGF gene enhances bone tissue angiogenesis. Repair in osteonecrosis would be accelerated accordingly.</p>
Sujets)
Animaux , Lapins , Collagène , Utilisations thérapeutiques , Tête du fémur , Métabolisme , Nécrose de la tête fémorale , Thérapeutique , Facteur de croissance fibroblastique de type 2 , Génétique , Expression des gènes , Thérapie génétique , Néovascularisation physiologique , Ostéogenèse , Plasmides , Protéines recombinantes , Génétique , TransfectionRésumé
<p><b>OBJECTIVE</b>To evaluate the effects of transforming growth factor beta1 (TGFbeta1) autocrine blockage on proliferation activity and drug sensitivity of osteosarcoma. METHODS; Northern blot, MTT determination, and 3H thymidine incorporation were used to investigate the effects of antisense TGF beta1 gene on osteosarcoma.</p><p><b>RESULTS</b>The proliferation of osteosarcoma cells transfected by antisense TGF beta1 gene was suppressed markedly, and adriamycin sensitivity was significantly increased.</p><p><b>CONCLUSION</b>Blockage of osteosarcoma cells TGF beta1 autocrine loop inhibits cell proliferation and enhances chemotherapy sensitivity.</p>
Sujets)
Humains , Antinéoplasiques , Pharmacologie , Éléments antisens (génétique) , Génétique , Communication autocrine , Tumeurs osseuses , Métabolisme , Anatomopathologie , Division cellulaire , Lignée cellulaire tumorale , Doxorubicine , Pharmacologie , Ostéosarcome , Métabolisme , Anatomopathologie , ARN messager , Génétique , Transfection , Facteur de croissance transformant bêta , Génétique , Facteur de croissance transformant bêta-1Résumé
<p><b>OBJECTIVE</b>To elucidate the effects of exogenous basic fibroblast growth factor (bFGF) on biological characteristics of rat osteoblasts cultured in vitro.</p><p><b>METHODS</b>The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-beta(1)) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts.</p><p><b>RESULTS</b>bFGF (5-50 ng/ml) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-beta(1) mRNA increased significantly, but the intracellular ALP content decreased.</p><p><b>CONCLUSIONS</b>bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-beta(1), but cannot promote the differentiation of osteoblasts.</p>