1.
Indian J Exp Biol
;
2001 Jan; 39(1): 95-7
Article
Dans Anglais
| IMSEAR
| ID: sea-60278
Résumé
A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-mediated destaining procedure, the present method is economical and user-friendly.