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Journal of Biomedical Engineering ; (6): 395-399, 2010.
Article Dans Chinois | WPRIM | ID: wpr-341611

Résumé

The epithelial growth factor receptor interference (EGFRi) was obtained by synthetic primers. Overlapping PCR was used to produce EGFRi-IL-24 fusion gene, which is linked by Gly4Ser3. After sequence analysis, EGFRi-IL-24 was cloned into expression vector pPIC9k; EGFRi-IL-24/pPIC9k was linearized with SacI,and then transformed to electroporated pastoris GS115. Subsequently, positive clone was selected by G418 and PCR, and its phenotype was determined by SDS-PAGE and MTT assay. The results demonstrated that EGFRi-IL-24 protein was expressed and shown to have the potential for use in researches of its biological function and in clinical application.


Sujets)
Humains , Antinéoplasiques , Pharmacologie , Vecteurs génétiques , Génétique , Interleukines , Génétique , Pichia , Génétique , Métabolisme , Récepteurs ErbB , Génétique , Protéines de fusion recombinantes , Génétique , Pharmacologie
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