Analysis on chemical constituents in Hedera nepalensis by UPLC/Q-TOF-MS/MS / 中草药

Objective: Using UPLC/Q-TOF-MS/MS to analyze and identify the chemical constituents from the stems and leaves of Hedera nepalensis. Methods: The analysis was performed on an Welch C18 reverse phase column (100 mm×2.1 mm, 1.7 μm). The mobile phase consisted of acetonitrile (B) and 0.1% formic acid (A) was used as gradient elute. The flow rate was 0.3 mL/min gradient elution and column temperature was 40℃, the injection volume was 5 uL, MS conditions were ESI, positive and negative ion mode scanning. Results: Under the optimized condition, based on the database of Scifinder, MS/MS of standards and compared with reference results, 43 compounds were identified, including triterpenoid saponins, flavonoid glycosides, phenylpropanoids, and nucleotides. Conclusion: By using UPLC/Q-TOF-MS/MS method the main chemical constituents from H. nepalensis can be rapidly and accurately identified.

Chemical constituents from the fruits of Embelia laeta / 中国药学杂志

OBJECTIVE: To study the chemical constituents of the fruits of Embelia laeta. METHODS: The compounds were isolated and purified by MCI, medium pressure TLC on silica gel column, ODS column chromatography, semi-preparative HPLC, and so on. The structures were elucidated on the basis of various modern spectroscopic techniques using physical, chemical properties and spectral data. RESULTS: Fifteen compounds were isolated and identified as nantenine(1), oxonantenine(2), physcion(3), syringic acid(4), vanillic acid(5), stigmast-4-ene-3,6-dione(6), (+)-lyoniresinol(7), 75,85-threo-4,7,9,9'-tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolignan(8), 1,3-dihydroxylpropyl-(9Z,12Z)-octadeca-9,12-dienate(9),(22E)-5a,8a-epidioxyergosta-6,22-dien-3b-ol(10), dihydroxyisoechinulin A(ll), hydroxybenzoic acid(12), stigmasterol(13), sitosterol(14), and daucosterol(15). CONCLUSION: Compounds 1,2, and 5-13 are isolated from the genus Embelia for the first time, and compounds 1-15 are isolated from this plant for the first time.

Rapid identification of the different constituents in Fructus Schisandrae Chinensis before and after processing by UHPLC-QTOF/MSE combining with metabonomics / 药学学报

This study was performed to use UHPLC-QTOF/MSE technology to rapidly search and identify variations of chemical ingredients between Fructus Schisandrae Chinensis and its processed products. The present study provides a basis for the study of Chinese herbal medicine processing with a focus on the impact of processing on chemical components. Using a time-dependent data scan mode (MSE) couple with metabolomics technology, we acquired accurate data and identified the potential chemical markers. A total of 12 chemical markers were identified in the crude, vinegar-processed and wine-processed Schisandra chinensis fruit; The results showed that the levels of 6-O-benzoylgomisin O, schisantherin B, schisantherin C, schisantherin D and neokadsuranic acid are the highest in crude Schisandra chinensis fruit; thelevels of schizandrin A, schizandrin B, schizandrin C, gomisin D and gomisin T are the highest in wine-processed Schisandra chinensis fruit; the levels ofschisantherin A and schisandrin are the highest in vinegar-processed Schisandra chinensis fruit. There were significant changes of chemical components between Fructus Schisandrae Chinensis and their processed products, and these findings may offer a reasonable explanation for variation of efficacy and clinical applications in the processed products of Fructus Schisandrae Chinensis.

Comparison on contents of four active constituents between slab and branch barks of Eucommia ulmoides / 中草药

Objective: To determine the contents of aucubin, chlorogenic acid, geniposide, and pinoresinol diglucoside in the slab and branch barks of Eucommia ulmoides. Methods: The separation was performed on a Cosmosil C18 (250 mm ×4.6 mm, 5 μm) column with the gradient elution acetonitrile -0.1% phosphoric acid; The flow rate was 0.8 mL/min; The detection wavelength was 208 nm; The column temperature was at 25 ℃. Results: The average content of aucubin: slab bark > branch bark; The average content of chlorogenic acid: branch bark > slab bark; The average content of geniposide: branch bark > slab bark; The average content of pinoresinol diglucosid: slab bark > branch bark. In different origins, the average contents of the above four constituents are more certain differences. Conclusion: The method is simple, rapid, accurate, and there are significant differences in the contents of aucubin, chlorogenic acid, geniposide, and pinoresinol diglucosid from the different parts of E. ulmoides, which would provide the better technique support for the quality control of E. ulmoides.