MiR-135a-5p suppresses breast cancer cell proliferation, migration, and invasion by regulating BAG3

Abstract Background MicroRNAs (miRNAs) are involved in the progression of diverse human cancers. This work aimed to delve into how microRNA-135a-5p (miR-135a-5p) affects the biological behaviors of Breast Cancer (BC) cells. Methods Gene Expression Omnibus (GEO) datasets were used to analyze the expression differences of miR-135a-5p in cancer tissues of BC patients. Quantitative real-time PCR and western blot were conducted to detect miR-135a-5p and Bcl-2 Associated Athanogene (BAG3) expression levels in BC tissues and cells, respectively. The proliferation, migration, invasion, and cell cycle of BC cells were detected by cell counting kit-8 assay, BrdU assay, wound healing assay, transwell assay, and flow cytometry. The targeted relationship between miR-135a-5p and BAG3 mRNA 3′UTR predicted by bioinformatics was further testified by a dual-luciferase reporter gene assay. Pearson's correlation analysis was adopted to analyze the correlation between miR-135a-5p expression and BAG3 expression. The downstream pathways of BAG3 were analyzed by the LinkedOmics database. Results MiR-135a-5p was significantly down-regulated and BAG3 expression was significantly raised in BC tissues. MiR-135a-5p overexpression repressed the viability, migration and invasion of BC cells, and blocked cell cycle progression in G0/G1 phase while inhibiting miR-135a-5p worked oppositely. BAG3 was verified as a target of miR-135a-5p. Overexpression of BAG3 reversed the impacts of miR-135a-5p on the malignant biological behaviors of BC cells. The high expression of BAG3 was associated with the activation of the cell cycle, mTOR and TGF-β signaling pathways. Conclusion MiR-135a-5p regulates BAG3 to repress the growth, migration, invasion, and cell cycle progression of BC cells.

Analysis of trace elements in the deaf-muted children among ethnic minorities in Urumchi / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To explore the relationship between trace elements and auditory function in the deafness children among ethnic minorities in Urumchi.</p><p><b>METHODS</b>There were 27 deaf-muted children in this study and 30 children with normal hearing as control. Sample of serum and hair from two groups were collected, and we tested 17 kinds of trace elements by using atomic absorption spectrophotometer and inductively coupled plasma emission spectrometer.</p><p><b>RESULTS</b>The content of trace elements in serum including Zn, Cu, Fe, Cd, Se, V and Pb from deaf-muted group was more higher than that of control group (P < 0.01), however, other trace elements including Mn, Sr, Sn, and Bi was much lower than that of control group (P < 0.01). Then, the content of those trace elements including Ba, Cr, Ag, Co, Ti, and Ni in serum from both testing and control groups was found no significant difference with each other (P > 0.05). On the other hand, the content of the trace elements including Sn and Se in hairs from deaf-muted group was more higher than that of control group (P < 0.05), but the content of the trace elements including Zn, Fe, Co, Mn, Sr, V, Pb, Ni, Cr, Ti and Ba in hairs from deaf-muted group was much lower than that of control group (P < 0.05), while the content of the trace element including Cu, Ag, Cd, and Bi in hairs from deaf-muted group was found no significant difference between each other (P > 0.05).</p><p><b>CONCLUSIONS</b>The content of some trace elements in serum and hairs of deaf-muted children is found a significant difference compared with children with normal hearing. This study indicates that some trace elements may relate to auditory function among ethnic minorities in Urumchi.</p>

Molecular cloning and characterization of a novel human kinase gene, PDIK1L.

We isolated a 4301-bp cDNA from a human foetal brain cDNA library by high-throughput cDNA sequencing. It encodes a protein of 341 amino acids, which shows 69% identity with the human kinase CLIK1 (AAL99353), which was suggested to be the CLP-36 interacting kinase. Bioinformatics analysis suggests that the putative kinase may interact with PDZ and LIM domain proteins. Therefore the protein and its cDNA were named 'PDLIM1 interacting kinase 1 like' (PDIK1L; nomenclature approved by the HUGO Gene Nomenclature Committee). Ensembl Genome Browser located PDIK1L to human chromosome 1p35.3. It spans about 13.7 kb and consists of four exons and three introns. Multiple-tissue cDNA panel PCR revealed that the gene is expressed widely in human tissues: liver, kidney, pancreas, spleen, thymus and prostate. The protein appears to be localized to the nucleus.