Résumé
Manganese related jobs [Mn miners] may cause Manganism, a Parkinson like disease. Chelators are suggested to reduce these effects. This study was carried out to investigate the effect of 4-Amino Salicylic acid chelating effects. This experimental study was done on 20 adult male Wistar rats. The rats were classified into four groups, 5 in each group, as follows: one control and three experimental groups. In two phases the groups received Salin-Salin, Manganese-Salin, Salin-4-Amino acid and Manganese -4-Amini acid, respectively. Eight mg/kg of manganese chloride i.p. injection for 1 week and 1.5g/l of PAS s.c injection five-day in a week for four weeks, were used for intoxication and treatment, respectively. Serum and liver tissues were separated. Data were analyzed using ANOVA and Tukey's tests. Biochemical factors showed cholesterol increment in group 2 compared with group 1 and decrement of cholesterol level in the blood of group 4 compare with group 2 [P<0.01]. Also increasing the direct billirubin in the Mn and PAS groups was more significant compared with control group [P<0.05]. Billirubin was observed in the urine of the Mn, PAS, Mn+PAS groups and urobilinogen declining was decreased in the urine of these groups [P<0.05]. There was a significant increase [P<0.05] of OT, PT enzymes in the serum of PAS and ALP in the Mn and Mn+PAS groups compared with control group. PAS can play a significant role in reducing destructive effects of Mn, especially on liver but has different side effects, like other chemical drugs
Résumé
Silver nanoparticles with excellent antimicrobial effects and increasing consumption in different industries are the most widely used nanoparticles and so it is necessary to evaluate the safety of their use. This study has examined the effects of time and consumption dose of these particles on rats of Vistar race. 36 Vistar race rats after 4 weeks equalization period divided into 6 groups [one control and five tests] and were received 95ppm, 65ppm, 35ppm, 20ppm, 5ppm concentrations of silver nano [Nanocid L2000] solution from NANONASB-E-PARS CO. as water. After 3 and 6 months 3 animals of each group were selected and hormonal parameters were determined by CLIA method, Hb and HCT was measured by common Med. Lab. Kits and blood cells counted by H1 equipment. Finally, results were processed by one-side variance and tukey's test. The results indicated that white blood cells counting show meaningful dose dependent decreasing in number of these cells. No tangible change was seen on counting of red blood cells and the level of hemoglobin and hematocrit parameters. Also blood cortisol and T4 level of dependent dose decreased and Testosterone increased meaningfully. Water uptake index increasing happened in meaningful and dose dependent manner. Ketone bodies appeared in rats' urine that had eaten high doses of silver nano. WBC count decreasing after oral consumption of silver nano particles show cell apoptosis and immunity lowering. Also the particles have no effect on bone marrow function indexes. On the other hand, lowering the function of adrenal gland, hypothyroidism, increasing in sexual hormone level are side effects of these particles consumption. In addition, increasing in Water Uptake Index and urine volume can be for high blood pressure or kidney failure caused by these particles