Résumé
yielded an average particle size of 120 nm with 70% encapsulation-efficiency. In vitro release profile of NP-OP showed sustained release of OP for 21 days. In vivo anti-fertility studies were conducted in marmosets. Results indicated that control animals conceived in the same cycle while two of three treated animals failed to conceive in treatment cycle. The in vivo studies thus corroborate with in vitro release of OP, demonstrating its anti-fertility activity in 66% of animals.
Sujets)
Animaux , Callithrix/physiologie , Protéines de transport/administration et posologie , Protéines de transport/composition chimique , Contraception , Femelle , Humains , Nanoparticules/administration et posologie , Nanoparticules/composition chimique , Follicule ovarique/composition chimique , Taille de particule , Fragments peptidiques/administration et posologie , Fragments peptidiques/composition chimique , Polymères/administration et posologie , Polymères/composition chimiqueRésumé
A synthetic nonapeptide, which is C-terminal sequence of 94-amino acid of prostatic inhibin peptide was tested for progesterone and estrogen secretion by mouse granulosa cell cultures. Nonapeptide suppressed the progesterone and estrogen synthesis, the magnitude of suppression was highest at 5 ng dose level for progesterone and 50 ng dose level for estradiol. The study suggests that, nonapeptide exerts its effect by impairing the binding of FSH to granulosa cell receptors.
Sujets)
Animaux , Cellules cultivées , Relation dose-effet des médicaments , Femelle , Hormone folliculostimulante/métabolisme , Hormones sexuelles stéroïdiennes/biosynthèse , Gonadotrophine équine , Cellules de la granulosa/effets des médicaments et des substances chimiques , Inhibines/pharmacologie , Souris , Fragments peptidiques , Progestérone/biosynthèseRésumé
Synthesis and biological profile of a decapeptide analogue, [Tyr85, Cys(Acm)87]85-94 of human seminal plasma inhibin (HSPI) are described. The peptide suppressed the circulatory levels of follicle stimulating hormone (FSH) in adult male rats. No change in the levels of luteinizing hormone (LH) and prolactin (Prl) was observed. Whereas the peptide suppressed the release of both FSH and LH in vitro. This decapeptide is the smallest peptide reported so far to have FSH suppressing activity.