Manufacture of MAC-ELISA applied in diagnosis of measles IgG antibody

Study serum of 443 health adults over 20 years old, negativeness with HIV and HBV and serum of 408 health childrens from 2 to 10 years old in Ha noi, Hai Phong and Thanh Hoa. Measles antigen were refined from Vero cell causing infection of Edmonton strain by Gradi extra centrifugal method with Sucrose density 20-60%. HA value of 1/11,024 showed high antigen specificity. 6 bunchs of protein were correllated with specific polypeptid strings of measles virus. After that, technical process and MAC ELISA-IgG were completed. Results: rate of measles immune response in adults was 100%, in which IgG value was high in 24%, moderate in 73.1% and low in 2.8%. Rate of serum IgG value in children was different to adults: positive serum 88%, negative 8.5%. Negative rate showed that the vaccine boosters were necessary

Using PCR technique to multiply NS4 gene of hepatitis C virus by deep vent DNA polymerase enzyme

Process of multiply NS4 gene of hepatitis C virus by PCR technique used with cDNA supplied from ARN. Couple of specific preys for NS4 gene and catalytic enzyme was Deep Vent ADN polymerase. Suitable MgSO4 level to multiply NS4 was 4mM. Fasten prey temperature was factor affecting to specificity of product. Result of electrophoresis showed that the most suitable temperature was 55oC and 57OC. In 200 µM dNTPs level, PCN product was sharp and correct to primary predictable size, in 50 or 100 µM dNTPs level, PCR wasn’t viewed, in 150 µM level, PCR was more blear than using 200 µM level. So dNTPs level was 200 µM

The value of AFP with lectin affinity in diagnosis, differentiated diagnosis, and in assessment of the effectiveness of treatment of HCC

In this study, the usefulness of the lectin reactive alpha-fetoprotein (AFP-L3) in patients with HCC, CLD and patients with HCC treated by transcatheter oil chemoembolization (TOCE) was examined. AFP-L3 levels were measured by lectins affinity electrophoresis and antibody affinity blotting. After TOCE, 40 HCC patients were classified into two groups: 1st group (n=27 with AFP-L315%. Results: The mean value of AFP-L3 in the 110 HCC patients was 54.6+/-23.7%, significantly higher (p<0.001) than the value in CLD (11.2+/-3.1%). When the cut-off level was set 15% for AFP-L3, the sensitivity was 96.4%, and the specificity was 95%. Survival rates after 12 and 18 months for all 27 patients calculated according to the Kaplan-Meier methods were 88.9% and 63% in the 1st group and 38.5% and 15.3% in the 2nd group, respectively. The recurrence of HCC for 1st and 2nd group were 10.2+/-3.5 months and 12.0+/-1.7 months. Conclusions: AFP-L3 is a useful marker for the diagnosis of HCC and for differentiation of increased AFP in HCC and CLD. AFP-L3 could be useful for the evaluation of curability after TOCE.

Purification of Lectin (Abrus precatorius) and its application in detecting pathogenic bacteria

Lectin (APA) was purified from jequirity bean (Abrus precatorius) by affinity chromatography on insoluble ovomucoid. Lectin (APA) and conjugate Lectin-biotin have been used in ELLA technique (Enzyme Linked Lectinosorbent Assay) to detect some pathogenic bacteria. Based on its specific bioavailability to the poD-Gal sugar compounds in cell membrane, lectin (APA) was capable of differentiating B. anthracis and B. cereus from S. flexneri, S. sonnei, C. diphteria, and Salmonella and Staphylococcus aureus.