Evaluation of wound healing effects between Salvadora a persica ointment and Solcoseryl jelly in animal model

In this research study very first time a herbal ointment contain 10% Salvadora persica extract was compared with Solcosseryl jelly 10% and blank Vaseline to evaluate wound healing effects using excision wound healing model in animals. Three groups of rats [n-6] were experimentally wounded on the back of their neck. Group I was dressed with Vaseline containing 10% test drug, Group II was treated with thin layer of Solcoseryl jelly 10% as reference drug while Group III was dressed with thin layer of blank Vaseline as control group. The effect of vehicle on rate of wound healing were assessed and in all cases there were progressive decreased in wound area with time but wound dress with Vaseline containing S. persica extract and wound treated with Solcosseryl jelly significantly healed earlier than those treated with Vaseline. It is concluded that S. persica extract significantly enhance the acceleration rate of wound enclosure in rats

Pharmacological rational of dry ripe fruit of Aegle marmelos L. as an anti-nociceptive agent in different painful conditions

The aim of study is to investigate central and peripheral analgesic effects of methanolic extract of dry ripe fruit of Aegle marmelos Linn. Corea [Am. Cr] by two methods, tail flick test and acetic acid induced writhing test at 100, 250 and 500mg/kg doses in animal models. Analgesic activity against tail flick test revealed that Am. Cr induced significant increase in latency period in dose dependent manner i.e. 65.38% at 100mg/kg, 395.37% at 250mg/kg [p<0.01] and 459.25% at 500mg/kg [p<0.01] body weight at 1hr after drug delivery while at 2hr effect decreased i.e. 61.53% at 100mg/kg, 161.11% [p<0.01] at 250mg/kg and 165.74% [p<0.01] at 500mg/kg but interestingly again there is an elongation in latency period at 3hr i.e. 106.15% at 100mg/kg dose, 251.85% [p<0.01] at 250mg/kg and 293.51% [p<0.05] at 500mg/kg respectively. The standard drug Diclofenac sodium at the dose of 5mg/kg continuously increased the latency period but less significantly as compared to the test substance i.e. 79.43%, 113.08% and 222.42% [p<0.05] respectively. Acetic acid induced writhing test produced highest significant activity at the dose of 100mg/kg i.e. 89.83% [p<0.01] as compared to Diclofenic sodium [standard drug] at a dose of 5mg/kg body weight i.e 63.63% [p<0.01]. It is concluded that dry ripe fruit of A. marmelos possesses significant dual analgesic activities i.e. central and peripheral

Evaluation of nutritional and sub-acute toxicological study of plant based supplement of Achyranthes aspera

The present study was conducted for the nutritional, microbiological and toxicological evaluation of test compound having main ingredient Achyranthes aspera

Nutritional value assessment, microbiological analysis and toxicological studies were conducted according to the standard reported methods which exhibited that A. aspera contains moisture 4.05%, proteins 20.54%, fats 0.903%, ash 20.25%, carbohydrates 54,26% and energy 294 Kcal. Vitamin profile was found to be B1 0.27mg/100g, B[2] 0.28mg/100g, B[3] 0.58mg/100g, B[6] 0.27mg/100g and B[9] 39microg/100g. The content of sodium, calcium, magnesium, potassium, chloride and phosphorus was found to be 1119.67, 5385.23, 5446.08, 1343.6, 675880.73 and 1447.5mg/kg respectively and trace metals i.e. iron, copper, zinc, manganese and aluminum were detected as 283.05, 8.062, 48.37, 16.12 and 9.853 mg/kg respectively

The microbiological result indicated that the compound qualifies the international standards of microbial limit and was found free from Salmonella species

The toxicological study was conducted to find safe use of Achyranthes aspera compound in human as a nutritive supplement in blood disorders

The toxicity studies exhibited that the test compound has a good effect on general health as an increase in body weights of animals of test group was noticed as compared to that of control group. Blood parameters before and after the study were monitored which confirms our hypothesis by showing an increase in hemoglobin from 9.133 to 10.96, RBC count from 3.11 to 3.6, WBC count from 5.68 to 5.73 and platelets from 245 to 319

Screening of solvent dependent antibacterial activity of Prunus domestica

Fruit of Prunus domestica was extracted in ethanol. The ethanol extract was further extracted with two solvents ethyl acetate and chloroform. The crude ethanol extract and two fractions [ethyl acetate and chloroform] were screened for their antibacterial activity using the agar well diffusion method. They were tested against nine bacteria; five Gram positive bacteria [Staphylococcus aureus, Streptococcuc intermedius, Bacillus cereus, Bacillus pumilus] and four Gram negative bacteria [Eschrichia coli, Proteus mirabilis Shigella flexneri, Salmonella typhi and Klebsiela pneumoniae]. The susceptibility of microorganisms to all three fractions was compared with each other and with standard antibiotic [Ampicillin] Among all fractions ethyl acetate exhibited highest antibacterial activity [average zone of inhibition 34.57mm +/- 1.3] while ethyl alcohol exhibited least antibacterial activity [average zone of inhibition 17.42mm +/- 3.3]. Minimum inhibitory concentration of ethanol, ethyl acetate and chloroform fractions was found in the range of 78ug/ml to 2500ugl/ml against gram positive and gram negative bacteria

Evaluation of analgesic activity of P. domestica L.

This research study was conducted to investigate acute oral toxicity and analgesic activity of ethanol extract of P. domestica fruit by using tail flick analgesiometer at 300 and 500mg/kg doses in animal models. Acute oral toxicity results showed that crude extract is safe up to the dose of 5g/kg body weight of animals. The analgesic activity revealed that P. domestica extract at 500mg/kg dose possesses highest significant and prolonged analgesic activity in dose dependent manner as compared to standard and control groups. Aspirin 300mg/kg body weight was used as standard drug. Phytochemical analysis was also carried out which showed the presence of certain phytochemicals constituents in test drug that are responsible for analgesic activity. Therefore the results are justified

Evaluation of antioxidant and urease inhibition activities of roots of Glycyrrhiza glabra

The object of this study is to determine the antioxidant activity of extracts from Glycyrrhiza glabra roots. The parent extract is methanolic extract while its sub fractions were prepared in ethyl acetate, chloroform, and n-butanol. The method based on scavenging activity and reduction capability of 1, 1-diphenyl-2-picrylhydrazyl radical [DPPH]. Urease inhibition activities of these extracts were also evaluated. Chloroform fraction was the most effective antioxidant with 87.7% activity but the activity is less than the crude methanolic extract i.e. 90%. Chloroform fraction showed the same trend in reducing power as that in radical scavenging activity. However n-butanol extract was devoid of any activity when compared to standard BHA. Crude methanolic fraction and its sub-fractions were also screened for enzyme inhibition activities using jackbean urease as substrate. Significant anti urease activity i.e. 72% was observed in the ethyl acetate fraction with respect to standard inhibitor thiourea

Pharmacological profile of salvadora persica

This work was conducted to investigate the various pharmacological activities of Salvadora. persica family Salvadoracea and that includes anti inflammatory, analgesic, CNS, bleeding and clotting time activity by oral administration at the dose of 300 and 500mg/kg of body weight in animal models. Acute oral toxicity results showed that crude extract of S. persica is safe up to the dose of 5g/kg body weight of animals. Carraganeen induced hind paw edema method for anti inflammatory activity, tail immersion test method for analgesic activity, Rota rod and grip strength test for CNS activity were carried out in animal models. The analgesic activity was compared with aspirin, 300mg/kg body weight, anti inflammatory activity was compared with indomethacine, lOmg/kg body weight, Transamin 250mg/kg and Vitamin K lOmg were used for bleeding and clotting time activity respectively while diazepam 5mg/kg were used as standard for behavior and CNS activities. In all activities S. persica showed prolonged and dose dependent effects. Phytochemical analysis was also carried out which showed the presence of certain phytoconstituents which possesses these properties. Therefore the results justified the traditional use of the plant

Studies on the effects of cyclodextrin polymer as a tableting aid on some selected analgesics

The clinical effectiveness of tablets depends on at least two factors; [i] the medication must be present in labeled amount [ii] it must be availble to the body. The drug availability is usually determined by the rate of release of drug from the tablet, which is governed by the processes of disintegration and dissolution. In present study, the effect of beta-Cyclodextrin on disintegration and rate of dissolution of analgesic tablets [paracetamol] have been studied. The results show that the tablets containing beta-Cyclodextrin polymer as a disintegrant enhances the rate of dissolution and reduces the disintegration time as compared to the commercially available tablets which lack beta-Cyclodextrin