Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 1 de 1
Filtre
Ajouter des filtres








Gamme d'année
1.
Mem. Inst. Oswaldo Cruz ; 112(2): 155-159, Feb. 2017. tab, graf
Article Dans Anglais | LILACS | ID: biblio-1040566

Résumé

BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. FINDINGS Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. MAIN CONCLUSION Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.


Sujets)
Animaux , Leishmania brasiliensis/effets des médicaments et des substances chimiques , Amphotéricine B/pharmacologie , Protéines à fluorescence verte/pharmacologie , Évaluation préclinique de médicament/méthodes , Antiprotozoaires/pharmacologie , Facteurs temps , Microscopie de fluorescence
SÉLECTION CITATIONS
Détails de la recherche