Effects of extracts from Hibiscus macranthus and Basella alba mixture on testosterone production in vitro in adult rat testes slices / 亚洲男科学杂志(英文版)

<p><b>AIM</b>To find an in vitro system for the measurement of the androgenic effects of different extracts of Hibiscus macranthus (Malvaceae) and Basella alba (Basellaceae).</p><p><b>METHODS</b>The production of testosterone from testes slices incubated in two media, either Krebs-Henseleit buffer containing 0.5% Bovine serum albumin (BSA) or Dubecco's Modified Eagle's medium-F12 Ham nutrient mixture (DME/Ham F12), under a mixture of 5% CO2 in 95% air was determined either in the presence or absence of cofactors and Hibiscus macranthus plus Basella alba (HMBA) extracts.</p><p><b>RESULTS</b>The testosterone production was increased in testes slices incubated in DME/Ham F12 medium in response to the cofactors (49%) and aqueous extracts (34%-60% according to dilutions). Under the same atmospheric conditions, there was no positive response of the testes slices to either cofactor or HMBA extract stimulation in Krebs-Henseleit buffer containing 0.5% BSA. In further investigations related to the effect of HMBA, the DME/Ham F12 medium was used. The results obtained from the in vitro test showed that the activity was present mainly in methylene chloride and methanol, since these extracts induced an increase in testosterone production by testes slices.</p><p><b>CONCLUSION</b>The testes slice system is suitable to be used for further in vitro investigations of the isolation of androgenic bioactive components of plants.</p>

Effects of Basella alba and Hibiscus macranthus extracts on testosterone production of adult rat and bull Leydig cells / 亚洲男科学杂志(英文版)

<p><b>AIM</b>To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull, and to develop a novel in vitro test system using Leydig cells from bull testes.</p><p><b>METHODS</b>The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the bull was analyzed using 125I-radioimmunoassay (125I-RIA). Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from bull testes.</p><p><b>RESULTS</b>Bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5-6 days, which can be stimulated by human chorionic gonadotrophin (hCG). Basella alba extracts significantly enhanced testosterone production in bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations.</p><p><b>CONCLUSION</b>Leydig cells purified from bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.</p>

Androgenic effect of Mondia whitei roots in male rats / 亚洲男科学杂志(英文版)

<p><b>AIM</b>To determine the effect of the aqueous extract of Mondia whitei (Periplocaceae) roots on testosterone production and fertility of male rats.</p><p><b>METHODS</b>Adult male Wistar rats were used. In the acute study, 20 rats were randomly divided into 5 groups of 4 animals each. Four treated groups were administered orally a single dose of Mondia whitei (400 mg/kg) and the controls received a similar amount of distilled water. One group of animals were sacrificed by cervical dislocation 1, 2, 4 and 6 h after treatment, respectively. The controls were sacrificed at 6 h. Testicular testosterone was determined by radioimmunoassay. In the chronic study, 28 rats were divided at random into 4 groups of 7 animals each: Groups 1, 2 and 3 were given orally the plant extract (400 mg.kg(-1).day(-1)) for 2, 4 and 8 days, respectively. The animals of Groups 1 and 2 were sacrificed 24 hours after the last dosing. The controls (Group 4) received the same amount of distilled water for 8 days. The fertility was assessed only in Groups 3 and 4 and after that, the animals were sacrificed and the epididymal sperm density, the serum testosterone and the testicular testosterone and 17 beta-estradiol were assayed. The serum, testicular and epidydimal protein contents were also determined.</p><p><b>RESULTS</b>In the acute treatment groups, the serum and testicular concentrations of testosterone remained unchanged at all the time points. Chronic treatment for 8 days induced a significant increase in the testicular weight, the serum and testicular testosterone, the testicular protein content and the sperm density (P < 0.05-0.01), but did not affect the accessory gland weights, the serum protein contents, the testicular concentration of 17beta -estradiol and the fertility compared to the controls.</p><p><b>CONCLUSION</b>Mondia whitei root extract possesses an androgenic property.</p>