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Asian Pacific Journal of Tropical Medicine ; (12): 645-650, 2012.
Article Dans Anglais | WPRIM | ID: wpr-819603

Résumé

OBJECTIVE@#To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma (NPC) cells.@*METHODS@#Pim-1 expressions in NPC cell lines CNE1, CNE1-GL, CNE-2Z and C666-1 were examined by RT-PCR, western blotting and immunoflucesence, respectively. After CNE1, CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor, quercetagetin, the cell viability, colony formation rate and migration ability were analyzed.@*RESULTS@#Pim-1 expression was negative in well-differentiated CNE1 cells, whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells. Interestingly, CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1. Treatment of CNE1-GL and C666-1 cells with quercetagetin significantly decreased the cell viability, colony formation rate and migration ability but not the CNE1 cells.@*CONCLUSIONS@#These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration, and targeting Pim-1 may be a potential treatment for anti-Pim-1-expressed NPCs.


Sujets)
Humains , Antinéoplasiques , Pharmacologie , Marqueurs biologiques tumoraux , Métabolisme , Technique de Western , Carcinomes , Lignée cellulaire tumorale , Mouvement cellulaire , Prolifération cellulaire , Survie cellulaire , 4H-1-Benzopyran-4-ones , Pharmacologie , Flavones , Technique d'immunofluorescence indirecte , Cancer du nasopharynx , Tumeurs du rhinopharynx , Métabolisme , Anatomopathologie , Protéines proto-oncogènes c-pim-1 , Métabolisme , RT-PCR , Test clonogénique de cellules souches tumorales
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