AQP5 gene silencing inhibits proliferation and migration of ectopic endometrial glandular epithelial cells in endometriosis / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the effect of aquaporin 5(AQP5) on proliferation and migration of ectopic endometrial epithelial cells.</p><p><b>METHODS</b>AQP5 shRNA interference fragments were designed and transfected into ectopic endometrial epithelial cells stably by lentivirus technology. Fluorescence quantitative RT-PCR and Western blotting were used to detect the AQP5 mRNA and protein expression, respectively. The cell proliferation and migration were determined by using MTT method and Transwell system, respectively. Levels of phosphorylated AKT(p-AKT) and total AKT were examined by Western blotting. The nude mice model of endometriosis was constructed and the endometrial cell nodule formation was observed.</p><p><b>RESULTS</b>AQP5 shRNA transfection inhibited cell proliferation and migration compared with control group (both P<0.05). The activation of AKT in AQP5 shRNA transfected cells was lower than that in control cells (P<0.01). Compared to control group, the endometrial cells nodule formation was suppressed in mice inoculated with AQP5 shRNA-silencing ectopic endometrial epithelial cells.</p><p><b>CONCLUSION</b>Down-regulation of AQP5 expression can suppress the proliferation and migration of ectopic endometrial epithelial cells and endometrial cell nodule formation in nude mice, in which AKT pathway may be involved.</p>

Effect of yangjing zhongyu decoction on matrix metalloproteinase-9 expression in endometrium and sex hormone regulation in women with cryptogenic infertility / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the effect of Yangjing Zhongyu decoction (YZD) on metalloproteinase-9 (MMP-9) and its inhibitor-1 (TIMP-1) expression and sex hormone regulation in mid-luteal phase endometrium of women with cryptogenic infertility.</p><p><b>METHODS</b>In situ hybridization and reverse transcription-polymerase chain reaction method was used to detect MMP-9 and TIMP-1 mRNA, and radioimmunoassay was used to determine levels of serum estradiol (E2) and progesterone (P) synchronously, of 22 infertile women during mid-luteal phase.</p><p><b>RESULTS</b>After treatment, the mid-luteal serum E2 and P level was 451.501 +/- 226.342 pmol/L and 46.502 +/- 19.948 nmol/L respectively, significantly higher than that before treatment (304.656 +/- 135.853 pmol/L and 33.782 +/- 15.459 nmol/L respectively), the difference was significant (P < 0.01). Staining of MMP-9 mRNA positive granules in cytoplasm and nuclei of adeno-epithelial cell mid-luteal phase endometrium deepened significantly, but the change in mesenchym was insignificant. The MMP-9 mRNA expression after treatment was 0.617 +/- 0.186 (grey level), significantly higher than the level before treatment (0.490 +/- 0.370), comparison between them showed significant difference (P < 0.05). Change of TIMP-1 mRNA expression in adeno-epithelial and mesenchym before and after treatment was insignificant (0.588 +/- 0.191 vs 0.621 +/- 0.146, P < 0.05). Correlation analysis showed that the quantitative difference of P value before and after treatment was positively correlated with the difference of MMP-9 mRNA before and after treatment (r = 0.682, P < 0.01).</p><p><b>CONCLUSION</b>YZD could soothen Gan and nourish Shen, raise the level of mid-luteal phase serum P, and further promote MMP-9 gene expression in endometrium to benefit the degradation of extracellular matrix of endometrium, and facilitate for blastocyst implantation.</p>

Effect of yangjing zhongyu decoction on expression of insulin-like growth factor II and its receptor in endometrium of women with unexplained infertility / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the effect of Yangjing Zhongyu Decoction (YJZYD) on expression of insulin-like growth factor II (IGF-II) and its receptor II (IGF-II R) in endometrium of women with unexplained infertility, and the relationship of which with the receptibility of endometrium to ovum implantation.</p><p><b>METHODS</b>Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect quantitatively the expression of IGF-II and IGF-II R in 22 women with unexplained infertility before and after YJZYD treatment during mid-luteal phase.</p><p><b>RESULTS</b>The levels of IGF-II and IGF-II R before treatment were 0.794 +/- 0.453 and 0.725 +/- 0.354 (in grey level, the same below) respectively, which were significantly increased in the same phase after treatment, reaching 1.202 +/- 0.551 and 1.045 +/- 0.581 respectively (P < 0.01 and P < 0.05). Correlation analysis showed the level of IGF-II mRNA was positively correlated with the level of IGF-II mRNA either before or after treatment.</p><p><b>CONCLUSION</b>YJZYD could enhance the expression of IGF-II and IGF-II R in the endometrium during mid-luteal phase, promote the differentiation of endometrium and increase its reception to ovum implantation.</p>

Endometrial insulin-like growth factor II and type I receptor in unexplained infertility and its steroid regulation / 浙江大学学报·医学版

OBJECTIVE: To investigate the endometrial expression of insulin-like growth factor II(IGF-II) and type I receptor(IGF-1R) in unexplained infertility. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect and quantify endometrial IGF-II and IGF-I R mRNA experession in the mid-luteal phase. Serum concentrations of estradiol(E(2)) and progesterone (P) were measured by radioimmunoassays. Thirty-four women with unexplained infertility were included in the study group. Twenty-one women with normal fertility were selected as the control group. RESULTS: Endometrial IGF-II and IGF-I R mRNA experession during the midluteal phase were significantly lower in the study group than in the control group (0.662 +/- 0.371 compared with 0.961+/- 0.389, P< 0.05; 0.582 +/- 0.257 compared with 0.829 +/-0.341, P< 0.05 respectively). The average serum P level in the study group was significantly lower than in the control group (23.782 +/-15.459 compared with 43.142 +/- 16.549nmol/L, P< 0.005). IGF-II mRNA expression correlated positively with IGF-I R mRNA expression in the two groups. Serum P level correlated positively with IGF-II and IGF-I R mRNA expression in the two groups. CONCLUSION: IGF-II and IGF-I R may play an important role in the implantation process. Their decreased expression relating to decreased P level may be important in unexplained infertility.