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An. acad. bras. ciênc ; 89(3,supl): 2053-2073, 2017. tab, graf
Article Dans Anglais | LILACS | ID: biblio-886784

Résumé

ABSTRACT This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC90) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization.


Sujets)
Humains , Animaux , Staphylococcus aureus/effets des médicaments et des substances chimiques , Extraits de plantes/pharmacologie , Enterococcus faecalis/effets des médicaments et des substances chimiques , Annona/composition chimique , Klebsiella pneumoniae/effets des médicaments et des substances chimiques , Antibactériens/pharmacologie , Tests de sensibilité microbienne , Membrane cellulaire/effets des médicaments et des substances chimiques , Chlorocebus aethiops , Lignée cellulaire tumorale/effets des médicaments et des substances chimiques
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